About: 18α-Glycyrrhetinic Acid Induces Apoptosis of HL-60 Human Leukemia Cells through Caspases- and Mitochondria-Dependent Signaling Pathways

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: 18α-Glycyrrhetinic Acid Induces Apoptosis of HL-60 Human Leukemia Cells through Caspases- and Mitochondria-Dependent Signaling Pathways Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	18α-Glycyrrhetinic Acid Induces Apoptosis of HL-60 Human Leukemia Cells through Caspases- and Mitochondria-Dependent Signaling Pathways
Creator	Chung, Jing-Gung Huang, Yi-Chang Kuo, Chao-Lin Lin, Jen-Jyh Lu, Kung-Wen Wu, Ping-Ping Yang, Jiun-Long Wu, Sai-Chuen, Rick
Source	PMC
abstract	In this study we investigate the molecular mechanisms of caspases and mitochondria in the extrinsic and intrinsic signal apoptosis pathways in human leukemia HL-60 cells after in vitro exposure to 18α-glycyrrhetinic acid (18α-GA). Cells were exposed to 18α-GA at various concentrations for various time periods and were harvested for flow cytometry total viable cell and apoptotic cell death measurements. Cells treated with 18α-GA significantly inhibited cell proliferation and induced cell apoptosis in a dose-dependent manner, with an IC(50) value of 100 μM at 48 h. The cell growth inhibition resulted in induction of apoptosis and decreased the mitochondria membrane potential (ΔΨ(m)) and increased caspase-8, -9 and -3 activities. Furthermore, cytochrome c and AIF were released from mitochondria, as shown by western blotting and confirmed by confocal laser microscopy. Western blotting showed that 18α-GA increased the levels of pro-apoptotic proteins such as Bax and Bid and decreased the anti-apoptotic proteins such as Bcl-2 and Bcl-xl, furthermore, results also showed that 18α-GA increased Fas and Fas-L which are associated with surface death receptor in HL-60 cells. Based on those observations, the present study supports the hypothesis that 18α-GA-induced apoptosis in HL-60 cells involves the activation of the both extrinsic and intrinsic apoptotic pathways.
has issue date	2016-07-01(xsd:dateTime)
bibo:doi	10.3390/molecules21070872
bibo:pmid	27376261
has license	cc-by
sha1sum (hex)	5bf6b6685fadbbc4899faa052321c697190bbade
schema:url	https://doi.org/10.3390/molecules21070872
resource representing a document's title	18α-Glycyrrhetinic Acid Induces Apoptosis of HL-60 Human Leukemia Cells through Caspases- and Mitochondria-Dependent Signaling Pathways
has PubMed Central identifier	PMC6273602
has PubMed identifier	27376261
schema:publication	Molecules
resource representing a document's body	covid:5bf6b6685fadbbc4899faa052321c697190bbade#body_text
is schema:about of	named entity 'HUMAN' named entity 'INTRINSIC' named entity 'WESTERN BLOTTING' named entity 'GLYCYRRHETINIC ACID' named entity 'CELLS' named entity 'RESULTS' named entity 'OBSERVATIONS' named entity 'EXTRINSIC' named entity 'ASSOCIATED WITH' named entity 'CELL' named entity 'INVESTIGATE' named entity 'CONFIRMED BY' named entity 'AIF' named entity 'EXPOSURE TO' named entity 'FLOW CYTOMETRY' named entity 'STUDY' named entity 'PRO-' named entity 'MEASUREMENTS' named entity 'MITOCHONDRIA' named entity 'MOLECULAR' named entity 'BASED' named entity 'BID' named entity 'APOPTOSIS' named entity 'INCREASED' named entity 'VIABLE CELL' named entity 'INDUCTION OF APOPTOSIS' named entity 'PRESENT' named entity 'DOSE-DEPENDENT' named entity 'CONCENTRATIONS' named entity 'ACTIVITIES' named entity 'PROTEINS' named entity 'GLYCYRRHETINIC ACID' named entity 'SURFACE' named entity 'LEUKEMIA' named entity 'CASPASE-8' named entity 'CYTOCHROME C' named entity '100' named entity 'MEMBRANE POTENTIAL' named entity 'INHIBITED' named entity 'DECREASED' named entity 'INDUCED' named entity 'PATHWAYS' named entity 'CONFOCAL LASER MICROSCOPY' named entity 'MITOCHONDRIA' named entity 'HL-60' named entity 'CASPASES' named entity 'MOLECULES' named entity 'LEUKEMIA CELLS' named entity 'DEPENDENT' named entity 'VALUE' named entity 'TREATED WITH' named entity 'IN VITRO' named entity 'TOTAL' named entity '2818' named entity 'CASPASES' named entity 'SIGNAL' named entity 'HYPOTHESIS' named entity 'LEVELS' named entity 'BCL-XL' named entity 'ACTIVATION' named entity 'HUMAN' named entity 'CELL GROWTH INHIBITION' named entity 'MITOCHONDRIA MEMBRANE' named entity 'VARIOUS' named entity 'HL-60 CELLS' named entity 'SIGNALING PATHWAYS' named entity 'BAX' named entity 'CELL PROLIFERATION' named entity 'FAS' named entity 'FAS-L' named entity 'MECHANISMS' named entity 'APOPTOSIS' named entity 'APOPTOTIC CELL DEATH'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software