About: Interplay between SIN3A and STAT3 Mediates Chromatin Conformational Changes and GFAP Expression during Cellular Differentiation

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Interplay between SIN3A and STAT3 Mediates Chromatin Conformational Changes and GFAP Expression during Cellular Differentiation
Creator	Wang, Yi-Ting Chen, Yu-Ju Lin, Yu-Ping Yu, John Chen, Ya-Ling Cheng, Pei-Yi Kao, Cheng-Fu Lee, Yi-Ching Tai, Chia-Chen
Source	PMC
abstract	BACKGROUND: Neurons and astrocytes are generated from common neural precursors, yet neurogenesis precedes astrocyte formation during embryogenesis. The mechanisms of neural development underlying suppression and de-suppression of differentiation- related genes for cell fate specifications are not well understood. METHODOLOGY/PRINCIPAL FINDINGS: By using an in vitro system in which NTera-2 cells were induced to differentiate into an astrocyte-like lineage, we revealed a novel role for Sin3A in maintaining the suppression of GFAP in NTera-2 cells. Sin3A coupled with MeCP2 bound to the GFAP promoter and their occupancies were correlated with repression of GFAP transcription. The repression by Sin3A and MeCP2 may be an essential mechanism underlying the inhibition of cell differentiation. Upon commitment toward an astrocyte-like lineage, Sin3A- MeCP2 departed from the promoter and activated STAT3 simultaneously bound to the promoter and exon 1 of GFAP; meanwhile, olig2 was exported from nuclei to the cytoplasm. This suggested that a three-dimensional or higher-order structure was provoked by STAT3 binding between the promoter and proximal coding regions. STAT3 then recruited CBP/p300 to exon 1 and targeted the promoter for histone H3K9 and H3K14 acetylation. The CBP/p300-mediated histone modification further facilitates chromatin remodeling, thereby enhancing H3K4 trimethylation and recruitment of RNA polymerase II to activate GFAP gene transcription. CONCLUSIONS/SIGNIFICANCE: These results provide evidence that exchange of repressor and activator complexes and epigenetic modifications are critical strategies for cellular differentiation and lineage-specific gene expression.
has issue date	2011-07-11(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0022018
bibo:pmid	21779366
has license	cc-by
sha1sum (hex)	5c364a85b8e920e9b4ad2fa201589fc319bc174b
schema:url	https://doi.org/10.1371/journal.pone.0022018
resource representing a document's title	Interplay between SIN3A and STAT3 Mediates Chromatin Conformational Changes and GFAP Expression during Cellular Differentiation
has PubMed Central identifier	PMC3136934
has PubMed identifier	21779366
schema:publication	PLoS One
resource representing a document's body	covid:5c364a85b8e920e9b4ad2fa201589fc319bc174b#body_text
is schema:about of	named entity 'Chromatin' named entity 'CELLULAR DIFFERENTIATION' covid:arg/5c364a85b8e920e9b4ad2fa201589fc319bc174b named entity 'SPECIFICATIONS' named entity 'EMBRYOGENESIS' named entity 'COMMON' named entity 'FORMATION' named entity 'BACKGROUND' named entity 'GENERATED' named entity 'NOT WELL' named entity 'MECHANISMS' named entity 'GFAP' named entity 'GENES' named entity 'DIFFERENTIATION' named entity 'CHROMATIN' named entity 'MEDIATES' named entity 'NEUROGENESIS' named entity 'EXPRESSION' named entity 'CHANGES' named entity 'STAT3' named entity 'UNDERLYING' named entity 'SUPPRESSION' named entity 'CELL FATE' named entity 'UNDERSTOOD' named entity 'NEURAL' named entity 'ASTROCYTE' named entity 'SIN3A' named entity 'RELATED' named entity 'ASTROCYTES' named entity 'NEURAL DEVELOPMENT' named entity 'NEURONS' named entity 'PRECURSORS' named entity 'mechanisms' named entity 'genes' named entity 'Differentiation' named entity 'Expression' named entity 'neural development' named entity 'neurogenesis' named entity 'Chromatin' named entity 'Cellular Differentiation' named entity 'Interplay' named entity 'transcription co-activator' named entity 'epigenetic regulation' named entity 'signaling pathway' named entity 'astrocyte' named entity 'MeCP2' named entity 'NTera-2' named entity 'exon' named entity 'differentiated cells' named entity 'olig2' named entity 'HDAC' named entity 'protoplasmic' named entity 'propidium iodide' named entity 'phosphoproteins' named entity 'Oligo' named entity 'Durham, NC' named entity 'acetyl' named entity 'lentiviral vector' named entity 'SYBR Green' named entity 'GFAP' named entity 'cell division' named entity 'coding region' named entity 'Promega' named entity 'acetylated' named entity 'immunofluorescence' named entity '1:50' named entity 'NTera-2' named entity 'exon' named entity 'RNAi' named entity 'statistical analysis' named entity 'clones' named entity '1:500' named entity 'GFAP'

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