About: Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294

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About: Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294
Creator	Zhang, Yanping Li, Kai Gao, Li Cui, Hongyu Wang, Xiaomei Wang, Yongqiang Gao, Honglei Gao, Yulong Guan, Xiaolu Liu, Changjun Liu, Yongzhen Qi, Xiaole Yun, Bingling Gao, Yanni
Source	PMC
abstract	Avian metapneumovirus (aMPV) and human metapneumovirus (hMPV) are members of the genus Metapneumovirus in the subfamily Pneumovirinae. Metapneumovirus fusion (F) protein mediates the fusion of host cells with the virus membrane for infection. Trypsin- and/or low pH-induced membrane fusion is a strain-dependent phenomenon for hMPV. Here, we demonstrated that three subtypes of aMPV (aMPV/A, aMPV/B, and aMPV/C) F proteins promoted cell-cell fusion in the absence of trypsin. Indeed, in the presence of trypsin, only aMPV/C F protein fusogenicity was enhanced. Mutagenesis of the amino acids at position 100 and/or 101, located at a putative cleavage region in aMPV F proteins, revealed that the trypsin-mediated fusogenicity of aMPV F proteins is regulated by the residues at positions 100 and 101. Moreover, we demonstrated that aMPV/A and aMPV/B F proteins mediated cell-cell fusion independent of low pH, whereas the aMPV/C F protein did not. Mutagenesis of the residue at position 294 in the aMPV/A, aMPV/B, and aMPV/C F proteins showed that 294G played a critical role in F protein-mediated fusion under low pH conditions. These findings on aMPV F protein-induced cell-cell fusion provide new insights into the molecular mechanisms underlying membrane fusion and pathogenesis of aMPV.
has issue date	2015-10-26(xsd:dateTime)
bibo:doi	10.1038/srep15584
bibo:pmid	26498473
has license	cc-by
sha1sum (hex)	5c5088ded6a49170c4ee50c8a9bfc5422cc31668
schema:url	https://doi.org/10.1038/srep15584
resource representing a document's title	Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294
has PubMed Central identifier	PMC4620442
has PubMed identifier	26498473
schema:publication	Sci Rep
resource representing a document's body	covid:5c5088ded6a49170c4ee50c8a9bfc5422cc31668#body_text
is schema:about of	named entity 'DEMONSTRATED' named entity 'PROTEINS' named entity 'PH-' named entity 'F PROTEIN' named entity 'MEMBRANE' named entity 'PRESENCE OF' named entity 'AMINO ACIDS' named entity '101' named entity 'CLEAVAGE' named entity 'ENHANCED' named entity 'MEDIATES' named entity 'THESE' named entity 'MOLECULAR' named entity 'SUBTYPES' named entity 'DEPENDENT' named entity 'REVEALED' named entity 'INSIGHTS' named entity 'FUSION' named entity 'NEW' named entity 'INDEPENDENT OF' named entity 'PATHOGENESIS' named entity 'OPEN' named entity 'ROLE' named entity 'ABSENCE OF' named entity 'REGION' named entity 'HMPV' named entity 'RESIDUE' named entity 'DID' named entity 'MEMBRANE FUSION' named entity 'CRITICAL' named entity 'STRAIN' named entity 'POSITIONS' named entity 'PH-' named entity 'MEDIATED' named entity '100%' named entity '101' named entity 'TRYPSIN' named entity 'LOW PH' named entity 'CONDITIONS' named entity 'METAPNEUMOVIRUS' named entity 'MUTAGENESIS' named entity 'CELL-CELL FUSION' named entity 'HERE' named entity 'PROTEIN ' named entity 'PHENOMENON' named entity 'AVIAN METAPNEUMOVIRUS' named entity 'FUSION' named entity 'PROVIDE' named entity 'INDUCED' named entity '100' named entity '2FB' named entity 'HOST CELLS' named entity 'MEDIATED' named entity 'SUBFAMILY PNEUMOVIRINAE' named entity 'FINDINGS' named entity 'POSITION' named entity 'DETERMINED BY' named entity 'PROTEINS' named entity 'VIRUS' named entity 'MECHANISMS' named entity 'UNDERLYING' named entity 'IS A' named entity 'TRYPSIN' named entity 'INFECTION' named entity 'REGULATED' named entity 'PLAYED' named entity 'LOW PH' covid:arg/5c5088ded6a49170c4ee50c8a9bfc5422cc31668

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