About: Establishment of Stable Cell Lines With High Expression of Heterodimers of Human 4F2hc and Human Amino Acid Transporter LAT1 or LAT2 and Delineation of Their Differential Interaction With α-Alkyl Moieties

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About: Establishment of Stable Cell Lines With High Expression of Heterodimers of Human 4F2hc and Human Amino Acid Transporter LAT1 or LAT2 and Delineation of Their Differential Interaction With α-Alkyl Moieties Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment of Stable Cell Lines With High Expression of Heterodimers of Human 4F2hc and Human Amino Acid Transporter LAT1 or LAT2 and Delineation of Their Differential Interaction With α-Alkyl Moieties
Creator	Kanai, Yoshikatsu Khunweeraphong, Narakorn Nagamori, Shushi Nishinaka, Yumiko Ohgaki, Ryuichi Sakurai, Hiroyuki Tanaka, Hidekazu Tominaga, Hideyuki Wiriyasermkul, Pattama Wongthai, Printip
Source	PMC
abstract	System L is a major transport system for cellular uptake of neutral amino acids. Among system L transporters, L-type amino acid transporter 1 (LAT1) is responsible for the nutrient uptake in cancer cells, whereas L-type amino acid transporter 2 (LAT2) is a transporter for non-cancer cells. In this study, we have established HEK293 cell lines stably expressing high levels of human LAT1 and LAT2 forming heterodimers with native human 4F2hc of the cells. We have found that l-[(14)C]alanine is an appropriate substrate to examine the function of LAT2, whereas l-[(14)C]leucine is used for LAT1. By using l-[(14)C]alanine on LAT2, we have for the first time directly evaluated the function of human LAT2 expressed in mammalian cells and obtained its reliable kinetics. Using α-alkyl amino acids including α-methyl-alanine and α-ethyl-l-alanine, we have demonstrated that α-alkyl groups interfere with the interaction with LAT2. These cell lines with higher practical advantages would be useful for screening and analyzing compounds to develop LAT1-specific drugs that can be used for cancer diagnosis and therapeutics. The strategy that we took to establish the cell lines would also be applicable to the other heterodimeric transporters with important therapeutic implications.
has issue date	2019-06-11(xsd:dateTime)
bibo:doi	10.1254/jphs.12124fp
bibo:pmid	22850614
has license	no-cc
sha1sum (hex)	5d119a4cad45a112b332047e35127b78608e5a10
schema:url	https://doi.org/10.1254/jphs.12124fp
resource representing a document's title	Establishment of Stable Cell Lines With High Expression of Heterodimers of Human 4F2hc and Human Amino Acid Transporter LAT1 or LAT2 and Delineation of Their Differential Interaction With α-Alkyl Moieties
has PubMed Central identifier	PMC7128428
has PubMed identifier	22850614
schema:publication	J Pharmacol Sci
resource representing a document's body	covid:5d119a4cad45a112b332047e35127b78608e5a10#body_text
is schema:about of	named entity 'demonstrated' named entity 'transport system' named entity 'diagnosis' named entity 'alanine' named entity 'screening' covid:arg/5d119a4cad45a112b332047e35127b78608e5a10 named entity 'function' named entity 'compounds' named entity 'cancer' named entity 'transporters' named entity 'time' named entity 'amino acids' named entity 'methyl' named entity 'amino acid' named entity 'alanine' named entity 'cell lines' named entity 'L-type' named entity 'alanine' named entity 'LAT1' named entity 'HEK293' named entity 'L-type' named entity 'implications' named entity 'transporter' named entity 'LAT2' named entity '1.2' named entity 'protein' named entity 'LAT1' named entity 'LAT1' named entity 'Leucine' named entity 'Protease inhibitor' named entity 'LAT1' named entity 'L-proline' named entity 'HEK293' named entity 'LAT2' named entity 'LAT1' named entity 'methyl' named entity 'alanine' named entity 'peroxidase' named entity 'L-leucine' named entity 'Oslo' named entity 'LAT1' named entity 'LAT2' named entity 'supernatants' named entity 'K i' named entity 'ovary' named entity 'HEK293' named entity 'cell lines' named entity 'antibodies' named entity 'LAT1' named entity 'plasma membrane' named entity 'LAT1' named entity 'melphalan' named entity 'LAT2' named entity '4F2hc' named entity 'SDS-PAGE' named entity 'LAT2' named entity 'HEK293' named entity 'kidney' named entity 'L-asparagine' named entity 'Kidney' named entity 'alanine' named entity 'HEK293' named entity 'HEK293' named entity 'renal proximal tubules' named entity 'gabapentin' named entity 'leucine' named entity 'LAT2' named entity 'substrates' named entity 'methyl' named entity 'LAT1' named entity 'Invitrogen' named entity 'LAT2'

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