About: Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses

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About: Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses
Creator	Kang, Bo-Kyu Park, Bong-Kyun Park, Seong-Jun Luo, Yuzi Ae, Kim Ae, Lee Ae, Moon Ae, Song Chul-Seung, A Dae-Sub, A Hye-Kwon, A Hyoung-Joon, A Kim, Eun-Mi Yang, Ae Jeong-Sun
Source	Medline; PMC
abstract	The open reading frame (ORF3) genes of the parent DR13, attenuated DR13, KPED-9, P-5V, and 12 field samples were cloned and sequenced to further explore the functions of wild- and attenuated-type porcine epidemic diarrhea viruses (PEDVs). Sequencing revealed that wild-type PEDVs ORF3 genes had a single ORF of 675 nucleotides encoding a protein of 224 amino acids with a predicted M (r) of 25.1–25.3 kDa. Attenuated-type PEDVs ORF3 genes had a single ORF of 624 nucleotides encoding a protein of 207 amino acids with a predicted M (r) of 23.4 kDa. The coding region of the ORF3 gene of attenuated-type PEDVs including attenuated DR13, KPED-9, and P-5V had 51 nucleotide deletions that were not found in the ORF3 genes of wild-type PEDVs including CV777, Br1/87, LZC, parent DR13, and 12 field samples. In addition, attenuated-type PEDVs have previously been found to exhibit reduced pathogenicity in pigs. Therefore, 51 nucleotide deletions appear to be meaningful and may be significant for PEDV pathogenicity, because they lead to changes in the predicted amino acid sequences of attenuated-type PEDVs. Reverse transcriptase-polymerase chain reaction (RT-PCR) on the partial ORF3 gene including 51 nucleotide deletions revealed that all PEDVs fell into two types, wild- and attenuated-type PEDVs. Wild-type PEDVs containing parent DR13 and 12 field samples had RT-PCR products of 245 bp in size, while attenuated-type PEDVs containing PEDV vaccine strains (attenuated DR13, KPED-9, P-5V) had products of 194 bp. In addition, all PEDV vaccine strains were used as live virus vaccine, because they previously exhibited a reduced pathogenicity in pigs. Therefore, large deletion region, which is comprise 17 amino acid deletions caused by 51 nucleotide deletions and is seen in all PED live vaccine strains, may be important site for PEDV pathogenicity, and we can use it for differentiation of wild- and attenuated-type PEDVs.
has issue date	2007-10-12(xsd:dateTime)
bibo:doi	10.1007/s11262-007-0164-2
bibo:pmid	17932736
has license	no-cc
sha1sum (hex)	5dbdae1a2d7e86db80696e2c26571c3f1138dd3e
schema:url	https://doi.org/10.1007/s11262-007-0164-2
resource representing a document's title	Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses
has PubMed Central identifier	PMC7088598
has PubMed identifier	17932736
schema:publication	Virus Genes
resource representing a document's body	covid:5dbdae1a2d7e86db80696e2c26571c3f1138dd3e#body_text
is schema:about of	named entity 'TYPE' named entity 'vaccine' named entity 'site' named entity 'chain reaction' named entity 'deletion' named entity 'RT-PCR' named entity 'addition' named entity 'functions' named entity 'Therefore' named entity 'field' named entity 'viruses' named entity 'ATTENUATED' named entity 'AMINO ACID' named entity '23.4' named entity 'AMINO ACID SEQUENCES' named entity 'TYPE' named entity 'PARTIAL' named entity 'LIVE' named entity 'PIGS' named entity 'TYPES' named entity 'PREVIOUSLY' named entity 'CODING REGION' named entity 'IMPORTANT' named entity 'FELL' named entity 'SINGLE' named entity 'LEAD' named entity 'NUCLEOTIDE' named entity 'PEDV' named entity 'VACCINE' named entity 'DIFFERENTIATION' named entity 'PROTEIN ' named entity 'VIRUSES' named entity 'REDUCED' named entity 'FUNCTIONS' named entity 'PCR' named entity 'MAY BE' named entity 'REVEALED' named entity 'ENCODING' covid:arg/5dbdae1a2d7e86db80696e2c26571c3f1138dd3e named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'SEQUENCE ANALYSIS' named entity 'USED' named entity 'PRODUCTS' named entity '624' named entity 'WILD-TYPE' named entity 'SIGNIFICANT' named entity 'HAVE' named entity 'KDA' named entity 'LARGE' named entity 'LIVE VACCINE' named entity 'SAMPLES' named entity 'SIZE' named entity 'CHANGES' named entity 'INCLUDING' named entity 'ORF3' named entity 'FOUND' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'CLONING' named entity 'SEEN' named entity '675' named entity 'CAUSED BY' named entity 'STRAINS' named entity 'A PROTEIN' named entity 'RT-PCR' named entity 'WILD' named entity 'GENE' named entity 'VIRUSES' named entity 'SITE'

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