About: Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies

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About: Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies
Creator	Nitsche, Andreas Hohn, Oliver Schade, Rüdiger Bourquain, Daniel Witkowski, Peter
Source	Elsevier; Medline; PMC
abstract	Abstract Orthopoxviruses code for numerous immunomodulatory proteins, the structure and function of which are clarified inadequately. Antibodies constitute a potent tool to study such proteins, enabling conclusions on protein location and time course of expression. However, common antibody production in mice or rabbits requires tedious protein expression and injection, as well as blood collection at regular intervals. To simplify this procedure, IgY antibodies specific for poxviral proteins (F1L and p28) were generated by immunisation of chickens, because antibody retrieval from eggs allows the non-invasive generation of huge amounts of antibodies. The main intentions were (i) to decrease invasiveness, (ii) to immunise with native forms of proteins and (iii) to circumvent previous protein expression and purification. Therefore, chicken were immunised with DNA expression vectors coding for conserved domains of the selected proteins delivered for the first time by a gene gun. Four weeks after initial immunisation specific antibodies were found in the egg yolk as proven by immunofluorescence staining of poxvirus-infected cells. The specific IgY titre rose to 1:80,000 and was stable for more than 120 days. With this investigation we present an universal procedure for IgY design and production that can be applied for various issues in the future.
has issue date	2009-02-28(xsd:dateTime)
bibo:doi	10.1016/j.jim.2008.11.008
bibo:pmid	19100269
has license	els-covid
sha1sum (hex)	5de791dacf00eab043ec804d189a7781b1070add
schema:url	https://doi.org/10.1016/j.jim.2008.11.008
resource representing a document's title	Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies
has PubMed Central identifier	PMC7094492
has PubMed identifier	19100269
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:5de791dacf00eab043ec804d189a7781b1070add#body_text
is schema:about of	named entity 'IMMUNISATION' named entity 'GUN' named entity '120' named entity 'INVESTIGATION' named entity 'EGGS' named entity 'DAYS' named entity 'FUNCTION' named entity 'SIMPLIFY' named entity 'EXPRESSION' named entity 'PROTEIN EXPRESSION' named entity '28I' named entity 'ANTIBODIES' named entity 'DESIGN' named entity 'ISSUES' named entity 'ROSE' named entity 'CELLS' named entity 'IMMUNOFLUORESCENCE STAINING' named entity 'INFECTED' named entity 'TIME COURSE' named entity 'GENERATED' named entity 'NONINVASIVE' named entity 'STRAIGHTFORWARD' named entity 'CHICKEN' named entity 'DNA' named entity 'GENE' named entity 'DNA' named entity 'APPLIED' named entity 'CHICKENS' named entity 'PROTEINS' named entity 'CLARIFIED' named entity 'EGG YOLK' named entity 'SUPPORTED' named entity 'POXVIRUS' named entity 'SPECIFIC' named entity 'PRODUCTION' named entity 'IGY' named entity 'FUTURE' named entity 'AMOUNTS' named entity 'CONSERVED' named entity 'MICE' named entity 'ANTIBODIES' named entity 'PRESENT' named entity 'PURIFICATION' named entity 'MAIN' named entity 'TITRE' named entity 'IMMUNISATION' named entity 'TOOL' named entity 'EXPRESSION VECTORS' named entity 'ANTIBODY PRODUCTION' named entity 'RETRIEVAL' named entity 'GENERATION' named entity 'PROVEN' named entity 'COMMON' named entity 'STABLE' named entity 'STRUCTURE' named entity 'CONCLUSIONS' named entity 'NUMEROUS' named entity 'BLOOD COLLECTION' named entity 'STUDY' named entity 'IMMUNOMODULATORY' named entity 'DELIVERED' named entity 'A GENE' named entity 'POXVIRUS' named entity 'TO DECREASE' named entity 'ANTIBODIES SPECIFIC' named entity 'MORE THAN' named entity 'ALLOWS' named entity 'SPECIFIC' named entity 'REGULAR' named entity 'INJECTION' named entity 'WEEKS' named entity 'INVASIVENESS' named entity 'GUN' named entity 'CODING' named entity 'IGY' named entity 'INITIAL' named entity 'UNIVERSAL'

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