About: Production of Recombinant N Protein of Infectious Bronchitis Virus Using the Baculovirus Expression System and Its Assessment as a Diagnostic Antigen

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Production of Recombinant N Protein of Infectious Bronchitis Virus Using the Baculovirus Expression System and Its Assessment as a Diagnostic Antigen Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Production of Recombinant N Protein of Infectious Bronchitis Virus Using the Baculovirus Expression System and Its Assessment as a Diagnostic Antigen
Creator	Aydin, Ozge Bayraktar, Erhan Cetinkaya, Burhan Cizmecigil, Utku Cotton-Caballero, Maira Faburay, Bonto Gurel, Aydin Richt, Juergen Tarakci, Eda Turan, Nuri Yilmaz, Aysun Yilmaz, Huseyin
source	Medline; PMC
abstract	The avian coronavirus-infectious bronchitis virus (AvCoV-IBV) is recognized as an important avian pathogen, and new viral variants are a continuous threat to the poultry industry worldwide. Sensitive diagnostics and efficacious vaccines are necessary to combat IBV infections in chickens. The aim of this study was to produce recombinant N protein of IBV in the baculovirus system to use in ELISA diagnostic tests in order to enable the assessment of the sero-prevalence and risk of IBV infections in chickens in Turkey. For this, the gene encoding the N protein of the Beaudette strain of IBV was expressed using a recombinant baculovirus expression system. The recombinant N protein was purified using Ni-NTA affinity chromatography. An estimated 50-kDa recombinant protein corresponding to the expected molecular weight of IBV N including the 6xHis tag was detected using an anti-His monoclonal antibody. Specific immunoreactivity of the recombinant protein was confirmed by Western blot using antiserum obtained from vaccinated and naturally infected chicken from Turkey as well as using a monoclonal antibody raised against the N protein of the IBV Massachusetts strain. The results obtained with the in-house ELISA had high agreement with a commercial ELISA. Immunoreactivity analysis using antisera in Western blotting and the in-house ELISA suggests that the recombinant IBV N protein could be broadly cross-reactive with antisera produced against different IBV strains. We conclude that the recombinant baculovirus expressed IBV N protein could serve as a useful diagnostic antigen for detection of IBV infections in chickens by ELISA.
has issue date	2018-07-09(xsd:dateTime)
bibo:doi	10.1007/s12010-018-2815-2
bibo:pmid	29987628
has license	no-cc
sha1sum (hex)	5e0fff0d12badd81afba0098f997f5143c0005f1
schema:url	https://doi.org/10.1007/s12010-018-2815-2
resource representing a document's title	Production of Recombinant N Protein of Infectious Bronchitis Virus Using the Baculovirus Expression System and Its Assessment as a Diagnostic Antigen
has PubMed Central identifier	PMC7090399
has PubMed identifier	29987628
schema:publication	Appl Biochem Biotechnol
resource representing a document's body	covid:5e0fff0d12badd81afba0098f997f5143c0005f1#body_text
is schema:about of	named entity 'N PROTEIN' named entity 'recombinant' named entity 'order' named entity 'BACULOVIRUS EXPRESSION SYSTEM' named entity 'VACCINES' named entity 'INFECTIOUS BRONCHITIS VIRUS' covid:arg/5e0fff0d12badd81afba0098f997f5143c0005f1 named entity 'CHICKENS' named entity 'SENSITIVE' named entity 'AIM' named entity 'COMBAT' named entity 'N PROTEIN' named entity 'IMPORTANT' named entity 'DIAGNOSTIC ANTIGEN' named entity 'ORDER' named entity 'PATHOGEN' named entity 'PROTEIN ' named entity 'HTTPS' named entity 'PROTEIN ' named entity 'USE' named entity 'THREAT' named entity 'STUDY' named entity 'ITS' named entity 'PRODUCTION' named entity 'USING' named entity 'POULTRY INDUSTRY' named entity 'APPL' named entity 'TO PRODUCE' named entity 'AVIAN' named entity 'DIAGNOSTICS' named entity 'ENABLE' named entity 'DIAGNOSTIC TESTS' named entity 'CONTINUOUS' named entity 'SYSTEM' named entity 'WORLDWIDE' named entity 'NECESSARY' named entity 'RECOMBINANT' named entity 'ELISA' named entity 'AVIAN CORONAVIRUS' named entity '187' named entity 'NEW' named entity 'ASSESSMENT' named entity 'VARIANTS' named entity 'INFECTIONS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'RECOMBINANT' named entity 'BACULOVIRUS' named entity 'VIRAL' named entity 'ASSESSMENT' named entity 'study' named entity 'vaccines' named entity 'diagnostic tests' named entity 'system' named entity 'IBV' named entity 'protein' named entity 'infectious bronchitis virus' named entity 'nucleocapsid' named entity 'IBV' named entity 'Nidovirales' named entity 'feces' named entity 'IBV' named entity 'Turkey' named entity 'IBV' named entity 'sneezing' named entity 'ELISAs' named entity 'IBV' named entity 'serological' named entity 'nucleocapsid' named entity 'immunization' named entity 'Serum'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software