About: Isolation and Propagation of Coronaviruses in Embryonated Eggs

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About: Isolation and Propagation of Coronaviruses in Embryonated Eggs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and Propagation of Coronaviruses in Embryonated Eggs
Creator	Guy, James
Source	Medline; PMC
abstract	The embryonated egg is a complex structure comprised of an embryo and its supporting membranes (chorioallantoic, amniotic, yolk). The developing embryo and its membranes provide the diversity of cell types that are needed for successful replication of a wide variety of different viruses. Within the family Coronaviridae the embryonated egg has been used as a host system primarily for two avian coronaviruses within the genus Gammacoronavirus, infectious bronchitis virus (IBV) and turkey coronavirus (TCoV). The embryonated egg also has been shown to be suitable for isolation and propagation of pheasant coronavirus, a proposed member of the Gammacoronavirus genus. IBV and pheasant coronavirus replicate well in the embryonated chicken egg, regardless of inoculation route; however, the allantoic route is favored as these viruses replicate well in epithelium lining the chorioallantoic membrane, with high virus titers found in these membranes and associated allantoic fluids. TCoV replicates only in epithelium lining the embryo intestines and bursa of Fabricius, thus amniotic inoculation is required for isolation and propagation of this virus. Embryonated eggs also provide a potential host system for detection and characterization of other, novel coronaviruses.
has issue date	2014-12-18(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-2438-7_7
bibo:pmid	25720472
has license	no-cc
sha1sum (hex)	5e77c07596acbf98257f1aa272898f16f2b0ee0e
schema:url	https://doi.org/10.1007/978-1-4939-2438-7_7
resource representing a document's title	Isolation and Propagation of Coronaviruses in Embryonated Eggs
has PubMed Central identifier	PMC7121728
has PubMed identifier	25720472
schema:publication	Coronaviruses
resource representing a document's body	covid:5e77c07596acbf98257f1aa272898f16f2b0ee0e#body_text
is schema:about of	named entity 'family' named entity 'avian' named entity 'chorioallantoic membrane' named entity 'membranes' named entity 'successful' named entity 'ROUTE' named entity 'SYSTEM' named entity 'REQUIRED' named entity 'EGGS' named entity 'USED' named entity 'ITS' named entity 'AMNIOTIC' named entity 'NOVEL' named entity 'EMBRYO' named entity 'PHEASANT CORONAVIRUS' named entity 'ISOLATION' named entity 'SUPPORTING' named entity 'THESE' named entity 'SUITABLE' named entity 'INFECTIOUS BRONCHITIS VIRUS' covid:arg/5e77c07596acbf98257f1aa272898f16f2b0ee0e named entity 'FAMILY CORONAVIRIDAE' named entity 'CORONAVIRUSES' named entity 'COMPLEX' named entity 'YOLK' named entity 'GENUS' named entity 'REPLICATION' named entity 'VIRUSES' named entity 'POTENTIAL' named entity 'VARIETY' named entity 'PROPOSED' named entity 'EPITHELIUM' named entity 'DIFFERENT' named entity 'DEVELOPING' named entity 'PROPAGATION' named entity 'IS A' named entity 'MEMBRANES' named entity 'NEEDED' named entity 'DETECTION' named entity 'LINING' named entity 'TURKEY CORONAVIRUS' named entity 'BURSA OF FABRICIUS' named entity 'ASSOCIATED' named entity 'REPLICATE' named entity 'HOST' named entity 'AVIAN CORONAVIRUSES' named entity 'GENUS GAMMACORONAVIRUS' named entity 'ISOLATION' named entity 'CORONAVIRUSES' named entity 'EGGS' named entity 'CHAPTER' named entity 'STRUCTURE' named entity 'MEMBER OF' named entity 'CELL TYPES' named entity 'FOUND' named entity 'DIVERSITY' named entity 'INTESTINES' named entity 'PROVIDE' named entity 'GAMMACORONAVIRUS' named entity 'CHICKEN EGG' named entity 'EGG' named entity 'HIGH' named entity 'PROPAGATION' named entity 'REGARDLESS' named entity 'INOCULATION' named entity 'WIDE' named entity 'CHARACTERIZATION' named entity 'CHORIOALLANTOIC MEMBRANE' named entity 'VIRUS' named entity 'SUCCESSFUL' named entity 'replicate' named entity 'cell types' named entity 'IBV' named entity 'system' named entity 'coronavirus' named entity 'Coronaviridae' named entity 'Gammacoronavirus' named entity 'inoculation' named entity 'turkey coronavirus' named entity 'viruses' named entity 'infectious bronchitis virus'

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