About: Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti
Creator	Boncy, Jacques Sepúlveda, Nuno Tetteh, Kevin Ashton, Ruth Chang, Michelle Drakeley, Chris Eisele, Thomas Elismé, Tamara Existe, Alexandre Hamre, Karen Joseph, Vena Lemoine, Jean Mondélus, Gina Présumé, Jacquelin Rogier, Eric Stresman, Gillian Romilus, Thomas Druetz, Van Den Hoogen, Lotus
topic	covid:5ea98ab0b1e038a936fcda575f363d4eb1c729a6#this
source	PMC
abstract	Measuring antimalarial antibodies can estimate transmission in a population. To compare outputs, standardized laboratory testing is required. Here we describe the in-country establishment and quality control (QC) of a multiplex bead assay (MBA) for three sero-surveys in Haiti. Total IgG data against 21 antigens were collected for 32,758 participants. Titration curves of hyperimmune sera were included on assay plates, assay signals underwent 5-parameter regression, and inspection of the median and interquartile range (IQR) for the y-inflection point was used to determine assay precision. The medians and IQRs were similar for Surveys 1 and 2 for most antigens, while the IQRs increased for some antigens in Survey 3. Levey-Jennings charts for selected antigens provided a pass/fail criterion for each assay plate and, of 387 assay plates, 13 (3.4%) were repeated. Individual samples failed if IgG binding to the generic glutathione-S-transferase protein was observed, with 659 (2.0%) samples failing. An additional 455 (1.4%) observations failed due to low bead numbers (<20/analyte). The final dataset included 609,438 anti-malaria IgG data points from 32,099 participants; 96.6% of all potential data points if no QC failures had occurred. The MBA can be deployed with high-throughput data collection and low inter-plate variability while ensuring data quality.
has issue date	2020-01-24(xsd:dateTime)
bibo:doi	10.1038/s41598-020-57876-0
bibo:pmid	31980693
has license	cc-by
sha1sum (hex)	5ea98ab0b1e038a936fcda575f363d4eb1c729a6
schema:url	https://doi.org/10.1038/s41598-020-57876-0
resource representing a document's title	Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti
has PubMed Central identifier	PMC6981173
has PubMed identifier	31980693
schema:publication	Sci Rep
resource representing a document's body	covid:5ea98ab0b1e038a936fcda575f363d4eb1c729a6#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/5ea98ab0b1e038a936fcda575f363d4eb1c729a6
is schema:about of	named entity 'data points' named entity 'data' named entity 'antigens' named entity 'Titration' named entity 'bead' named entity 'population' named entity 'antigens' named entity '455' named entity 'DATA COLLECTION' named entity '659' named entity 'GLUTATHIONE' named entity 'REQUIRED' named entity 'PARAMETER' named entity 'MEDIAN' named entity 'IGG BINDING' named entity 'OCCURRED' named entity 'FINAL' named entity 'HERE' named entity 'INFLECTION POINT' covid:arg/5ea98ab0b1e038a936fcda575f363d4eb1c729a6 named entity 'determine' named entity 'Survey' named entity 'sera' named entity 'standardized' named entity 'numbers' named entity 'assay' named entity 'samples' named entity 'additional' named entity 'antimalarial' named entity 'failed' named entity 'collected' named entity 'interquartile range' named entity 'Here' named entity 'IgG' named entity 'data points' named entity 'interquartile range' named entity 'glutathione-S-transferase' named entity 'high-throughput' named entity 'pass/fail' named entity 'IgG' named entity 'IQR' named entity 'large-scale' named entity 'antibody' named entity 'multiplex' named entity 'Quality control' named entity 'assay' named entity 'precision' named entity 'standard curve' named entity 'antigens' named entity 'WHO' named entity 'Antigen' named entity 'w/v' named entity 'DBS' named entity 'sodium azide' named entity 'Study population' named entity 'P. falciparum' named entity 'Statistical analyses' named entity 'serum' named entity 'serological' named entity 'antigens' named entity 'Antigens'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software