About: Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs

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About: Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs
Creator	Cheng, Shipeng Cheng, Yuening Wang, Jianke Yi, Li Yang, Shen Luo, Bin Xu, Hongli
Source	Elsevier; Medline; PMC
abstract	Abstract A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and differentiated the CDV wild-type and vaccine strains by two separate RT-PCRs. The method can be used for clinical detection and epidemiological surveillance. The phylogenetic analysis of the hemagglutinin gene of the local wild-type CDV strains revealed that the seven local isolates all belonged to the Asia-1 lineage, and were clustered closely with one another at the same location. These results suggested that the CDV genotype Asia-1 is circulating currently in domestic dogs in China.
has issue date	2012-01-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2011.11.011
bibo:pmid	22108430
has license	els-covid
sha1sum (hex)	5f40139f8376dfa7abdc27536ea6090de68cdb6c
schema:url	https://doi.org/10.1016/j.jviromet.2011.11.011
resource representing a document's title	Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs
has PubMed Central identifier	PMC7112914
has PubMed identifier	22108430
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:5f40139f8376dfa7abdc27536ea6090de68cdb6c#body_text
is schema:about of	named entity 'SPECIFIC' covid:arg/5f40139f8376dfa7abdc27536ea6090de68cdb6c named entity 'DEVELOPMENT' named entity 'PROTOCOL' named entity 'HEMAGGLUTININ' named entity 'VACCINATED' named entity 'CANINE ADENOVIRUS' named entity 'CELLS' named entity 'LOCATION' named entity 'STRAINS' named entity 'TO DETECT' named entity 'RESULTS' named entity 'CDV' named entity 'DIFFERENTIATED' named entity 'DOGS' named entity 'SEPARATE' named entity 'GENOMIC' named entity 'PAIR' named entity 'PHYLOGENETIC ANALYSIS' named entity 'HEMAGGLUTININ' named entity 'CIRCULATING' named entity 'DETECTION' named entity 'COMBINED' named entity 'CANINE DISTEMPER VIRUS' named entity 'CANINE CORONAVIRUS' named entity 'COMBINED' named entity 'PRIMERS' named entity 'LINEAGE' named entity 'EPIDEMIOLOGICAL' named entity '555' named entity 'STRAINS' named entity 'DIFFERENTIATION' named entity 'RT-PCR' named entity 'SEVEN' named entity 'DOGS' named entity 'ANIMALS' named entity 'CHINESE' named entity 'CANINE DISTEMPER VIRUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'SEVEN' named entity 'REVEALED' named entity 'METHOD' named entity 'WILD-TYPE' named entity 'PCR' named entity 'GENE' named entity 'DEMONSTRATED' named entity 'GENETIC CHARACTERIZATION' named entity 'DETECTED' named entity 'INFECTED' named entity 'LOCAL' named entity 'VACCINE' named entity 'GENOTYPE' named entity 'CURRENTLY' named entity 'AMPLIFICATION' named entity 'GENE' named entity 'CLUSTERED' named entity 'ISOLATES' named entity 'INFECTED' named entity 'USED' named entity 'REVERSE-TRANSCRIPTION' named entity 'DIFFERENTIATION' named entity 'DOMESTIC' named entity 'CANINE PARVOVIRUS' named entity 'THESE' named entity 'CHINA' named entity 'CLINICAL' named entity 'VACCINES' named entity 'ASIA' named entity 'RNA' named entity 'SURVEILLANCE' named entity 'FRAGMENT' named entity '335' named entity 'AMPLIFIED' named entity 'UNINFECTED' named entity 'wild-type'

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