About: Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

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About: Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
Creator	Gao, Haixia Gao, Shuibo Li, Enyao
Source	PMC
abstract	Context: Morphine is an alkaloid isolated from the poppy plants. The addiction of morphine is a very serious social issue. Some long non-coding RNAs (lncRNAs) have been proposed to engage in drug addiction. Objective: Whether lncRNA maternally expressed gene 3 (MEG3) attended to morphine-mediated autophagy of mouse hippocampal neuronal HT22 cells was probed. Materials and methods: HT22 cells were subjected to 10 µM morphine for 24 h. Cell autophagy was assessed by measuring LC3-II/LC3-I and Beclin-1 expression. qRT-PCR was carried out to measure MEG3 expression. SiRNA oligoribonucleotides targeting MEG3 (si-MEG3) was transfected to silence MEG3. The orexin1 receptor (OX1R), c-fos, p/t-ERK and p/t-PKC expressions were tested by western blotting. SCH772984 was used as an inhibitor of ERK pathway. Results: Morphine elevated OX1R (2.92 times), c-fos (2.06 times), p/t-ERK (2.04 times) and p/t-PKC (2.4 times), Beclin-1 (3.2 times) and LC3-II/LC3-I (3.96 times) expression in HT22 cells. Moreover, followed by morphine exposure, the MEG3 expression was also elevated in HT22 cells (3.03 times). The silence of MEG3 lowered the Beclin-1 (1.85 times), LC3-II/LC3-I (2.12 times), c-fos (1.39 times) and p/t-ERK (1.44 times) expressions in morphine-treated HT22 cells. Inhibitor of ERK pathway SCH772984 further promoted the influence of MEG3 silence on morphine-caused Beclin-1 (1.97 times) and LC3-II/LC3-I (1.92 times) expressions decreases. Conclusions: Up-regulation of MEG3 attended to the morphine-caused autophagy of HT22 cells might be through elevating c-fos expression and promoting ERK pathway activation. More experiments are also needed in the future to analyse the influence of other lncRNAs in drug addiction.
has issue date	2019-08-21(xsd:dateTime)
bibo:doi	10.1080/13880209.2019.1651343
bibo:pmid	31433241
has license	cc-by-nc
sha1sum (hex)	5f56a9b712608c599a21191b06b78bfa70ad523f
schema:url	https://doi.org/10.1080/13880209.2019.1651343
resource representing a document's title	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
has PubMed Central identifier	PMC6713166
has PubMed identifier	31433241
schema:publication	Pharm Biol
resource representing a document's body	covid:5f56a9b712608c599a21191b06b78bfa70ad523f#body_text
is schema:about of	named entity '282' named entity 'OX1R' named entity 'INHIBITOR' named entity 'LONG NON-CODING RNAS' named entity 'CELLS' named entity 'CELL' named entity 'RESULTS' named entity 'EXPERIMENTS' named entity 'INFLUENCE' named entity 'ELEVATED' named entity 'CONCLUSIONS' named entity 'SOCIAL ISSUE' named entity 'PROMOTING' named entity 'PROBED' named entity 'HT22' named entity 'QRT-PCR' named entity 'NEURONAL' named entity '4 TIMES' named entity 'LNCRNA' named entity 'CONTEXT' named entity 'TESTED' named entity 'TIMES' named entity 'OLIGORIBONUCLEOTIDES' named entity 'HIPPOCAMPAL' named entity 'attended' named entity 'isolated' named entity 'qRT-PCR' named entity 'Some' named entity 'times' named entity 'cells' named entity 'expressed' named entity 'times' named entity 'cells' named entity 'western blotting' named entity 'promoted' named entity 'oligoribonucleotides' named entity 'experiments' named entity 'assessed' named entity 'ERK pathway' covid:arg/5f56a9b712608c599a21191b06b78bfa70ad523f named entity 'LONG NON-CODING RNA' named entity 'IS A' named entity 'CAUSED' named entity 'CARRIED' named entity 'OBJECTIVE' named entity 'PATHWAY' named entity 'MORPHINE' named entity 'MEG3' named entity 'MOUSE' named entity 'ADDICTION' named entity 'ALKALOID' named entity 'MORPHINE' named entity 'WESTERN BLOTTING' named entity 'TREATED' named entity 'PROPOSED' named entity 'TO MEASURE' named entity 'LOWERED' named entity 'C-FOS' named entity 'ATTENDS' named entity 'HT22' named entity 'MEDIATED' named entity 'MODULATING' named entity 'CELLS' named entity 'USED' named entity 'SCH772984' named entity '283' named entity 'ENGAGE' named entity 'POPPY' named entity 'ASSESSED' named entity 'ELEVATING' named entity 'LC3-II' named entity 'UP-REGULATION' named entity 'RECEPTOR' named entity '2 TIMES' named entity 'ERK' named entity 'EXPRESSION' named entity 'MATERIALS' named entity 'MEG3' named entity 'ERK'

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