About: Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus
Creator	Wang, Jian Li, Wei Yang, Ruifu Zhu, Qingyu Zhang, Ting Wang, Hao Liu, Siqi Xu, Ningzhi Zhang, Xumin Yang, Ling Yu, Jun Wen, Jie Yang, Huanming Li, Jingxiang Fang, Jianqiu He, Bo Huang, Shengyong You, Bo Wang, Jingqiang Zeng, Changqing Li, Xiaolei Yin, Jianning Yin, Ye Lei, Tingting Bhatnagar, Aruni Cui, Xiaodai De Qin, E Lu, Youyong Ma, Lianhua Tang, Xiangjun Wang, Luoping West, Matthew Yang, Yongkui
Source	Medline; PMC
abstract	Background: The widespread threat of severe acute respiratory syndrome (SARS) to human life has spawned challenges to develop fast and accurate analytical methods for its early diagnosis and to create a safe antiviral vaccine for preventive use. Consequently, we thoroughly investigated the immunoreactivities with patient sera of a series of synthesized peptides from SARS-coronavirus structural proteins. Methods: We synthesized 41 peptides ranging in size from 16 to 25 amino acid residues of relatively high hydrophilicity. The immunoreactivities of the peptides with SARS patient sera were determined by ELISA. Results: Four epitopic sites, S599, M137, N66, and N371-404, located in the SARS-coronavirus S, M, and N proteins, respectively, were detected by screening synthesized peptides. Notably, N371 and N385, located at the COOH terminus of the N protein, inhibited binding of antibodies to SARS-coronavirus lysate and bound to antibodies in >94% of samples from SARS study patients. N385 had the highest affinity for forming peptide-antibody complexes with SARS serum. Conclusions: Five peptides from SARS structural proteins, especially two from the COOH terminus of the N protein, appear to be highly immunogenic and may be useful for serologic assays. The identification of these antigenic peptides contributes to the understanding of the immunogenicity and persistence of SARS coronavirus.
has issue date	2003-12-01(xsd:dateTime)
bibo:doi	10.1373/clinchem.2003.023184
bibo:pmid	14633869
has license	no-cc
sha1sum (hex)	5f6de6299608fcce69212b0a26366621a119afbb
schema:url	https://doi.org/10.1373/clinchem.2003.023184
resource representing a document's title	Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus
has PubMed Central identifier	PMC7108193
has PubMed identifier	14633869
schema:publication	Clin Chem
resource representing a document's body	covid:5f6de6299608fcce69212b0a26366621a119afbb#body_text
is schema:about of	named entity 'severe acute respiratory syndrome (SARS)' named entity 'determined' named entity 'protein' named entity 'investigated' named entity 'residues' named entity 'hydrophilicity' named entity 'Results' named entity 'develop' named entity 'lysate' named entity 'Peptides' named entity 'PATIENTS' named entity 'VACCINE' named entity 'SCREENING' named entity 'METHODS' named entity 'LYSATE' covid:arg/5f6de6299608fcce69212b0a26366621a119afbb named entity 'ASSESSMENT' named entity 'DETECTED' named entity 'SERUM' named entity 'PEPTIDE' named entity 'HYDROPHILICITY' named entity 'SITES' named entity 'THOROUGHLY' named entity 'CORONAVIRUS' named entity 'N PROTEIN' named entity 'SAFE' named entity 'PEPTIDES' named entity 'STUDY' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ELISA' named entity 'PREVENTIVE' named entity 'SYNTHESIZED' named entity 'INHIBITED' named entity 'AFFINITY' named entity 'DETERMINED BY' named entity 'COMPLEXES' named entity 'BINDING' named entity 'EARLY DIAGNOSIS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'FORMING' named entity 'ANTIBODIES' named entity 'WIDESPREAD' named entity 'THREAT' named entity 'AMINO ACID' named entity 'ANALYTICAL METHODS' named entity 'HIGH' named entity 'ANTIVIRAL' named entity 'BOUND' named entity 'SERIES'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software