About: RNA and polypeptide homology among murine coronaviruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: RNA and polypeptide homology among murine coronaviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA and polypeptide homology among murine coronaviruses
Creator	Cheley, Steve Morris, Vincent Anderson', Robert Cupples, Margaret
Source	Elsevier; Medline; PMC
abstract	Abstract Using a 32P complementary DNA (cDNA) prepared against the A59 nucleocapsid protein messenger RNA, we have investigated the extent of homology between A59 and four other strains of murine hepatitis virus (MHV). Analysis by hybridization kinetics of the annealing between A59 [32P]cDNA and infected cell RNA from the other four MHV strains demonstrated 70–80% homology. By gel transfer analysis, the A59 [32P]cDNA was able to detect subgenomic-size virus-specific RNAs in cells infected with all of the five MHV strains. A similar pattern of seven viral RNAs was detected in cells infected with A59, MHV-1, MHV-3, and JHM. In contrast, cells infected with MHV-S contained seven virus-specific RNAs, of which only the two smallest species comigrated with RNAs from the other four strains. The results suggest that as previously shown with A59 (S. Cheley, R. Anderson, M. J. Cupples, E. C. M. Lee Chan, and V. L. Morris (1981) Virology, 112, 596–604), all MHV strains tested encode a nested set of subgenomic RNAs. Analysis of [35S]methionine-labeled viral proteins by SDS-polyacrylamide gel electrophoresis revealed that each strain of MHV specified four major viral polypeptides with apparent molecular weights very similar to those previously reported for the E2, N, El, and PEI polypeptides of A59. The strong degree of interstrain homology among the five MHV strains investigated was confirmed by comparative chymotryptic peptide mapping of the viral N proteins. A majority of the chymotryptic peptides from each of the [35Sknethionine-labeled N proteins was found to coelute by high-performance liquid chromotography. Moreover, this technique of peptide mapping indicated a particularly strong relatedness between MHV-1 and MHV-S and among MHV-3, JHM, and A59.
has issue date	1981-12-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(81)90113-6
bibo:pmid	7314448
has license	els-covid
sha1sum (hex)	6058e813e7b4c4f5c57eba44da2463bd5774574b
schema:url	https://doi.org/10.1016/0042-6822%2881%2990113-6
resource representing a document's title	RNA and polypeptide homology among murine coronaviruses
has PubMed Central identifier	PMC7133649
has PubMed identifier	7314448
schema:publication	Virology
resource representing a document's body	covid:6058e813e7b4c4f5c57eba44da2463bd5774574b#body_text
is schema:about of	named entity 'infected' named entity 'A59' named entity 'prepared' named entity 'infected' named entity 'MHV' named entity 'gel' named entity 'Polypeptide' named entity 'MURINE' named entity 'PREVIOUSLY' named entity 'CELLS' named entity 'MESSENGER RNA' named entity 'SET' named entity 'HYBRIDIZATION' named entity 'LEE' named entity 'SIZE' named entity 'SDS' named entity 'PROTEIN ' named entity 'SIMILAR' named entity '28S' named entity 'PATTERN' named entity 'INFECTED' named entity 'LABELED' named entity 'VIRAL' named entity 'POLYPEPTIDE' named entity 'CORONAVIRUSES' named entity 'USING' named entity 'MURINE HEPATITIS VIRUS' named entity 'VIRUS' named entity 'DEMONSTRATED' named entity 'SUGGEST' named entity 'NUCLEOCAPSID PROTEIN' named entity 'RNA' named entity 'EXTENT OF' named entity 'RESULTS' named entity 'MHV-S' named entity 'INVESTIGATED' named entity 'HOMOLOGY' named entity 'RNA' named entity 'ENCODE' named entity 'SMALLEST' named entity 'SPECIES' named entity 'CONTRAST' named entity 'HAVE' named entity 'TRANSFER' named entity 'HOMOLOGY' named entity '604' named entity 'NESTED' named entity 'CONTAINED' named entity 'TESTED' named entity 'DETECTED' named entity 'GEL' named entity 'INFECTED CELL' named entity 'VIRAL PROTEINS' named entity 'ANNEALING' named entity 'SPECIFIC' named entity 'STRAINS' named entity 'POLYACRYLAMIDE' named entity 'SEVEN' named entity 'ANALYSIS' named entity 'PREPARED' named entity 'TO DETECT' named entity 'MHV-1' named entity 'KINETICS' named entity '112' named entity 'ANDERSON' named entity 'A59' named entity 'COMPLEMENTARY DNA' covid:arg/6058e813e7b4c4f5c57eba44da2463bd5774574b named entity 'nested set' named entity 'A59' named entity 'viral' named entity 'annealing' named entity 'contained' named entity 'cDNA' named entity 'homology' named entity 'A59' named entity 'RNAs' named entity 'R. Anderson'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software