About: Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens

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About: Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens
Creator	Huang, He Pu, Juan Huang, Jin Wei, Kai Bielke, Lisa Chen, Ruichang Hu, Liping Miao, Yongqiang Niu, Xiangyun Sha, Zhou Shang, Hongqi Zhu, Ruiliang
Source	Medline; PMC
abstract	H9N2 subtype low pathogenicity avian influenza virus (LPAIV) is distributed worldwide and causes enormous economic losses in the poultry industry. Despite immunization of almost all chickens with inactivated vaccines, the disease still remains widespread. We speculated that improving mucosal or cellular immune responses could contribute to improved control of H9N2 viruses. In this study, we constructed a novel Lactococcus lactis (L. lactis) strain expressing a recombinant fusion protein consisting of the M1 and HA2 proteins derived from an antigenically conserved endemic H9N2 virus strain. The M1-HA2 fusion protein was cloned downstream of a gene encoding a secretory peptide, and we subsequently confirmed that the fusion protein was secreted from L. lactis by Western blotting. We assessed the immunogenicity and protective effects of this recombinant L. lactis strain. Eighty 1-day-old chickens were divided into four groups, and the experimental groups were orally vaccinated twice with the recombinant L. lactis strain. Fecal and intestinal samples, sera, and bronchoalveolar lavage fluid were collected at 7, 14, and 21 days post-vaccination (dpv). Chickens vaccinated with the recombinant L. lactis strain showed significantly increased levels of serum antibodies, T cell-mediated immune responses, and mucosal secretory IgA (SIgA). Following challenge with H9N2 virus at 21 dpv, chickens vaccinated with the recombinant L. lactis strain showed decreased weight loss, lower viral titers in the lung, and reduced lung pathological damage. In summary, our results demonstrated that a recombinant L. lactis strain expressing an H9N2 M1-HA2 fusion protein could induce protective mucosal and systemic immunity. This oral vaccine is H9N2 virus-specific and represents a significant design improvement compared with previous studies. Our study provides a theoretical basis for improving mucosal immune responses to prevent and control H9N2 virus infection.
has issue date	2020-03-24(xsd:dateTime)
bibo:doi	10.3389/fvets.2020.00153
bibo:pmid	32266297
has license	cc-by
sha1sum (hex)	60dc97efd7dd7a7397593669cfbe2f89b67d6af4
schema:url	https://doi.org/10.3389/fvets.2020.00153
resource representing a document's title	Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens
has PubMed Central identifier	PMC7105734
has PubMed identifier	32266297
schema:publication	Front Vet Sci
resource representing a document's body	covid:60dc97efd7dd7a7397593669cfbe2f89b67d6af4#body_text
is schema:about of	named entity 'CHICKENS' named entity 'control' named entity 'H9N2' named entity 'mucosal' named entity 'L. lactis' named entity 'proteins' named entity 'vaccinated' named entity 'chickens' named entity 'fusion protein' named entity 'experimental' named entity 'improving' named entity 'RECOMBINANT' covid:arg/60dc97efd7dd7a7397593669cfbe2f89b67d6af4 named entity 'LACTOCOCCUS LACTIS' named entity 'SYSTEMIC' named entity 'SERA' named entity 'INCREASED' named entity 'AVIAN INFLUENZA VIRUS' named entity 'PEPTIDE' named entity 'PREVIOUS' named entity 'CAUSES' named entity 'COMPARED' named entity 'WORLDWIDE' named entity 'REDUCED' named entity 'CHALLENGE' named entity 'BRONCHOALVEOLAR LAVAGE FLUID' named entity 'VIRUS INFECTION' named entity 'PROTEIN ' named entity 'ORAL VACCINE' named entity 'IMPROVED' named entity 'WIDESPREAD' named entity 'EFFECTS' named entity 'DAY-OLD' named entity 'WEIGHT LOSS' named entity 'CLONED' named entity 'POULTRY INDUSTRY' named entity 'EXPERIMENTAL' named entity 'COLLECTED' named entity 'CONSERVED' named entity 'DESIGN' named entity 'FECAL' named entity 'SPECIFIC' named entity 'TWICE' named entity 'LEVELS' named entity 'SUMMARY' named entity 'CONTROL' named entity 'T CELL' named entity 'FOLLOWING' named entity 'INDUCE' named entity 'POST' named entity 'TO PREVENT' named entity 'VIRUSES' named entity 'STRAIN' named entity 'IMMUNITY' named entity 'SAMPLES' named entity 'CONSTRUCTED' named entity 'HA2' named entity 'DIVIDED' named entity 'DECREASED WEIGHT' named entity 'DEMONSTRATED' named entity 'LOW' named entity 'ENDEMIC' named entity 'DOWNSTREAM' named entity 'CONTRIBUTE ' named entity 'LOWER' named entity 'VACCINATED' named entity 'OUR' named entity 'MUCOSAL' named entity 'INACTIVATED VACCINES' named entity 'PROVIDES'

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