About: Spread from the Sink to the Patient: In Situ Study Using Green Fluorescent Protein (GFP)-Expressing Escherichia coli To Model Bacterial Dispersion from Hand-Washing Sink-Trap Reservoirs

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About: Spread from the Sink to the Patient: In Situ Study Using Green Fluorescent Protein (GFP)-Expressing Escherichia coli To Model Bacterial Dispersion from Hand-Washing Sink-Trap Reservoirs Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Spread from the Sink to the Patient: In Situ Study Using Green Fluorescent Protein (GFP)-Expressing Escherichia coli To Model Bacterial Dispersion from Hand-Washing Sink-Trap Reservoirs
Creator	Chai, Weidong Barry, Katie Guilford, William Kotay, Shireen Kotay, Citation Mathers, Amy
Source	Medline; PMC
abstract	There have been an increasing number of reports implicating Gammaproteobacteria as often carrying genes of drug resistance from colonized sink traps to vulnerable hospitalized patients. However, the mechanism of transmission from the wastewater of the sink P-trap to patients remains poorly understood. Herein we report the use of a designated hand-washing sink lab gallery to model dispersion of green fluorescent protein (GFP)-expressing Escherichia coli from sink wastewater to the surrounding environment. We found no dispersion of GFP-expressing E. coli directly from the P-trap to the sink basin or surrounding countertop with coincident water flow from a faucet. However, when the GFP-expressing E. coli cells were allowed to mature in the P-trap under conditions similar to those in a hospital environment, a GFP-expressing E. coli-containing putative biofilm extended upward over 7 days to reach the strainer. This subsequently resulted in droplet dispersion to the surrounding areas (<30 in.) during faucet operation. We also demonstrated that P-trap colonization could occur by retrograde transmission along a common pipe. We postulate that the organisms mobilize up to the strainer from the P-trap, resulting in droplet dispersion rather than dispersion directly from the P-trap. This work helps to further define the mode of transmission of bacteria from a P-trap reservoir to a vulnerable hospitalized patient. IMPORTANCE Many recent reports demonstrate that sink drain pipes become colonized with highly consequential multidrug-resistant bacteria, which then results in hospital-acquired infections. However, the mechanism of dispersal of bacteria from the sink to patients has not been fully elucidated. Through establishment of a unique sink gallery, this work found that a staged mode of transmission involving biofilm growth from the lower pipe to the sink strainer and subsequent splatter to the bowl and surrounding area occurs rather than splatter directly from the water in the lower pipe. We have also demonstrated that bacterial transmission can occur via connections in wastewater plumbing to neighboring sinks. This work helps to more clearly define the mechanism and risk of transmission from a wastewater source to hospitalized patients in a world with increasingly antibiotic-resistant bacteria that can thrive in wastewater environments and cause infections in vulnerable patients.
has issue date	2017-03-31(xsd:dateTime)
bibo:doi	10.1128/aem.03327-16
bibo:pmid	28235877
has license	cc-by
sha1sum (hex)	615071c8c959f24857b1bad521cc432b59719bfb
schema:url	https://doi.org/10.1128/aem.03327-16
resource representing a document's title	Spread from the Sink to the Patient: In Situ Study Using Green Fluorescent Protein (GFP)-Expressing Escherichia coli To Model Bacterial Dispersion from Hand-Washing Sink-Trap Reservoirs
has PubMed Central identifier	PMC5377511
has PubMed identifier	28235877
schema:publication	Appl Environ Microbiol
resource representing a document's body	covid:615071c8c959f24857b1bad521cc432b59719bfb#body_text
is schema:about of	named entity 'RESERVOIRS' named entity 'dispersion' named entity 'environment' named entity 'splatter' named entity 'green fluorescent protein (GFP)' named entity 'areas' named entity 'transmission' named entity 'HAND' named entity 'ESCHERICHIA COLI' named entity 'PROTEIN ' named entity 'HAVE' named entity 'UPWARD' named entity 'AREAS' named entity 'REACH' named entity 'CELLS' named entity 'VULNERABLE' named entity 'SUBSEQUENT' named entity 'FAUCET' named entity 'MODE OF TRANSMISSION' named entity 'GREEN FLUORESCENT PROTEIN' named entity 'CARRYING' named entity 'ANTIBIOTIC-RESISTANT BACTERIA' named entity 'ALLOWED' named entity 'INVOLVING' named entity 'LOWER' named entity 'GENES' named entity 'ENVIRONMENT' named entity 'WASTEWATER' named entity 'MULTIDRUG-RESISTANT BACTERIA' named entity 'RESULTING IN' named entity 'GAMMAPROTEOBACTERIA' named entity 'CONNECTIONS' named entity 'BIOFILM' named entity 'PLUMBING' named entity 'SINK' named entity 'RESERVOIR' named entity 'TRAPS' named entity 'ESCHERICHIA COLI' named entity 'BASIN' named entity 'SOURCE' named entity 'DISPERSAL' named entity 'REPORT' named entity 'POORLY' named entity 'GFP' named entity 'HELPS' named entity 'CONDITIONS' named entity 'ENVIRONMENTS' named entity 'RETROGRADE' named entity 'INCREASING' named entity 'HOSPITAL ENVIRONMENT' named entity 'UNDERSTOOD' named entity 'UNIQUE' named entity 'ORGANISMS' named entity 'WATER' named entity 'WORK' named entity 'MATURE' named entity 'IN SITU' named entity 'TRAP' named entity 'WASHING' named entity 'STRAINER' named entity 'RECENT' named entity 'DROPLET' named entity 'GROWTH' named entity 'DIRECTLY' named entity 'UP TO' named entity 'NEIGHBORING' named entity 'OPERATION' named entity 'SPREAD' named entity 'SINK' named entity 'WASHING' named entity 'BACTERIA ' named entity 'PROTEIN ' named entity 'THE BOWL' named entity 'MODEL' named entity 'BACTERIAL' named entity 'DISPERSION'

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