About: NAADP-dependent Ca2+ signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: NAADP-dependent Ca2+ signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	NAADP-dependent Ca2+ signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system
Creator	Yang, Yang Li, Fang Gunaratne, Gihan Marchant, Jonathan Walseth, Timothy
Source	Elsevier; Medline; PMC
abstract	Abstract Middle East Respiratory Syndrome coronavirus (MERS-CoV) infections are associated with a significant mortality rate, and existing drugs show poor efficacy. Identifying novel targets/pathways required for MERS infectivity is therefore important for developing novel therapeutics. As an enveloped virus, translocation through the endolysosomal system provides one pathway for cellular entry of MERS-CoV. In this context, Ca2+-permeable channels within the endolysosomal system regulate both the luminal environment and trafficking events, meriting investigation of their role in regulating processing and trafficking of MERS-CoV. Knockdown of endogenous two-pore channels (TPCs), targets for the Ca2+ mobilizing second messenger NAADP, impaired infectivity in a MERS-CoV spike pseudovirus particle translocation assay. This effect was selective as knockdown of the lysosomal cation channel mucolipin-1 (TRPML1) was without effect. Pharmacological inhibition of NAADP-evoked Ca2+ release using several bisbenzylisoquinoline alkaloids also blocked MERS pseudovirus translocation. Knockdown of TPC1 (biased endosomally) or TPC2 (biased lysosomally) decreased the activity of furin, a protease which facilitates MERS fusion with cellular membranes. Pharmacological or genetic inhibition of TPC1 activity also inhibited endosomal motility impairing pseudovirus progression through the endolysosomal system. Overall, these data support a selective, spatially autonomous role for TPCs within acidic organelles to support MERS-CoV translocation.
has issue date	2018-11-30(xsd:dateTime)
bibo:doi	10.1016/j.ceca.2018.08.003
bibo:pmid	30121440
has license	els-covid
sha1sum (hex)	61ac93bdaec7585d407c80ad2f33708fac3977d9
schema:url	https://doi.org/10.1016/j.ceca.2018.08.003
resource representing a document's title	NAADP-dependent Ca2+ signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system
has PubMed Central identifier	PMC6251489
has PubMed identifier	30121440
schema:publication	Cell Calcium
resource representing a document's body	covid:61ac93bdaec7585d407c80ad2f33708fac3977d9#body_text
is schema:about of	named entity 'BLOCKED' named entity 'DEVELOPING' named entity 'MOTILITY' named entity 'MOBILIZING' named entity 'PATHWAY' named entity 'ORGANELLES' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ENTRY' named entity 'PHARMACOLOGICAL' named entity 'TPC1' named entity 'INHIBITION' named entity 'REQUIRED' named entity 'FUSION' named entity 'SIGNIFICANT' named entity 'TRAFFICKING' named entity 'PROGRESSION' named entity 'ENVIRONMENT' named entity 'TARGETS' named entity 'INVESTIGATION' named entity 'THEIR' named entity 'AUTONOMOUS' named entity 'INHIBITED' named entity 'Knockdown' named entity 'Pharmacological' named entity 'translocation' named entity 'infectivity' named entity 'organelles' named entity 'MERS-CoV' named entity 'infectivity' named entity 'motility' named entity 'regulates' named entity 'NAADP' named entity 'SYSTEM' named entity 'LUMINAL' named entity 'BISBENZYLISOQUINOLINE ALKALOIDS' named entity 'CATION CHANNEL' named entity 'CHANNELS' named entity 'MIDDLE EAST RESPIRATORY SYNDROME' named entity 'CORONAVIRUS' named entity 'PROVIDES' named entity 'REGULATE' named entity 'MERS' named entity 'PSEUDOVIRUS' named entity 'SECOND MESSENGER' named entity 'NAADP' named entity 'GENETIC' named entity 'SPIKE' named entity 'NOVEL THERAPEUTICS' named entity 'PERMEABLE' named entity 'SYSTEM' named entity 'EFFICACY' named entity 'REGULATING' named entity 'TO SUPPORT' named entity 'TRANSLOCATION' named entity 'DEPENDENT' named entity 'SIGNALING' named entity 'PSEUDOVIRUS' named entity 'PORE' named entity 'IDENTIFYING' named entity 'PARTICLE' named entity 'USING' named entity 'CELLULAR' named entity 'ASSOCIATED WITH' named entity 'OVERALL' named entity 'EVENTS' named entity 'MEMBRANES' named entity 'NOVEL' named entity 'POOR' named entity 'DATA' named entity 'INFECTIVITY' named entity 'THESE' named entity 'CONTEXT' named entity 'ACTIVITY' named entity 'PROCESSING' named entity 'INFECTIONS' named entity 'ACIDIC' named entity 'SUPPORT'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software