About: Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement

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About: Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement
Creator	Chen, Yung-Wu Dev, Vaithilingam Tucker, Philip Uhr, Jonathan Vitetta, Ellen Word, Charlotte
Source	PMC
abstract	In our accompanying paper, we described a switch variant (BCL1.2.58) that expresses membrane and secreted forms of IgM and IgG1. Both IgM and IgG1 share the same idiotype and use the same VDJ rearrangement. Here, a detailed Southern blot analysis of the entire constant region of the Ig heavy chain (Ig CH) locus of parental (BCL1.B1) and variants (BCL1.B2) DNA showed no detectable rearrangement. Similar analysis of the JH-C mu region led to the conclusion that two heavy chain alleles present in the IgM/IgG1-producing variants carried the same VDJ rearrangement but differed in their 3' flanking regions. One chromosome 12 did not carry any Ig CH genes, whereas, the other chromosome 12 carried one copy of CH genes. In BCL1.B1, however, each of the chromosome 12 alleles carried a full copy of CH genes. Karyotypic analysis confirmed the presence of two translocated t(12;16) chromosomes in both BCL1.2.58 and BCL1.B1 cells, with a break 5' to the VH locus at the distal region (12F2) of chromosome 12, and at the proximal region below the centromere (16B3) of chromosome 16. We conclude that double production of IgM and IgG1 in BCL1.B2 is accomplished by transcription of the corresponding CH genes in germline configuration using a single VDJ on the same chromosome 12.
has issue date	1986-08-01(xsd:dateTime)
bibo:pmid	3088208
has license	cc-by-nc-sa
sha1sum (hex)	61f4cc62aa5a8b65d63664101a7e4af71a7c1df4
resource representing a document's title	Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement
has PubMed Central identifier	PMC2188233
has PubMed identifier	3088208
schema:publication	J Exp Med
resource representing a document's body	covid:61f4cc62aa5a8b65d63664101a7e4af71a7c1df4#body_text
is schema:about of	named entity 'ISOTYPE' named entity 'DOUBLE' named entity 'NEOPLASTIC' named entity 'HEAVY' named entity 'CHAINS' named entity 'B CELL' named entity 'CELL LINE' named entity 'GENE REARRANGEMENT' named entity 'ALLELICALLY EXCLUDED' named entity 'LINE 11' named entity 'PRODUCTION' named entity 'allelic exclusion' named entity 'Bio-Rad' named entity 'cell lines' named entity 'splenic' named entity 'Kpn' named entity 'cloned' named entity 'BCL' named entity 'VDJ' named entity 'intron' named entity 'cytogenetics' named entity 'BCL' named entity 'germline' named entity 'BCL' named entity 'introns' named entity 'karyotype' named entity 'kbp' named entity 'Restriction endonucleases' named entity 'kbp' named entity 'VDJ' named entity 'IgM' named entity 'heavy chains' named entity 'BCL' named entity 'chromosome 12' named entity 'cDNA' named entity 'VDJ' named entity 'BCL' named entity 'Ig heavy chain' named entity 'locus' named entity 'IgM' named entity 'BCL' named entity 'IgD' named entity 'gene' named entity 'chromosome 12' named entity 'VDJ' named entity 'germline' named entity 'Fc receptors' named entity 'hybridization' named entity 'transcription' named entity 'Kpn' named entity 'secretion' named entity 'leukemia cells' named entity 'IgM' named entity 'chromosome 12' named entity 'leukemic' named entity 'IgM' named entity 'gene' named entity 'mRNA' named entity 'repetitive sequences' named entity 'intron' named entity 'Densitometry' named entity 'infection' named entity 'BCL' named entity 'nick translation' named entity 'alleles' named entity 'chromosome number' named entity 'distal' named entity 'BCL' named entity 'mRNA' named entity 'chromosome 12' named entity 'liver' named entity 'VDJ' named entity 'alleles' named entity 'Kpn' named entity 'IgM' named entity 'chromosomes' named entity 'germline' named entity 'FACS'

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