About: Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera

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About: Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera
Creator	Alonso, Covadonga Hernaez, Bruno Escribano, Jose
Source	Elsevier; Medline; PMC
abstract	Abstract Many stages of African swine fever virus infection have not yet been studied in detail. To track the behavior of African swine fever virus (ASFV) in the infected cells in real time, we produced an infectious recombinant ASFV (B54GFP-2) that expresses and incorporates into the virus particle a chimera of the p54 envelope protein fused to the enhanced green fluorescent protein (EGFP). The incorporation of the fusion protein into the virus particle was confirmed immunologically and it was determined that p54-EGFP was fully functional by confirmation that the recombinant virus made normal-sized plaques and presented similar growth curves to the wild-type virus. The tagged virus was visualized as individual fluorescent particles during the first stages of infection and allowed to visualize the infection progression in living cells through the viral life cycle by confocal microscopy. In this work, diverse potential applications of B54GFP-2 to study different aspects of ASFV infection are shown. By using this recombinant virus it was possible to determine the trajectory and speed of intracellular virus movement. Additionally, we have been able to visualize for first time the ASFV factory formation dynamics and the cytophatic effect of the virus in live infected cells. Finally, we have analyzed virus progression along the infection cycle and infected cell death as time-lapse animations.
has issue date	2006-06-20(xsd:dateTime)
bibo:doi	10.1016/j.virol.2006.01.021
bibo:pmid	16490226
has license	els-covid
sha1sum (hex)	61fa6a990e2ad4f339c27eeba2b345aae391143e
schema:url	https://doi.org/10.1016/j.virol.2006.01.021
resource representing a document's title	Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera
has PubMed Central identifier	PMC7111804
has PubMed identifier	16490226
schema:publication	Virology
resource representing a document's body	covid:61fa6a990e2ad4f339c27eeba2b345aae391143e#body_text
is schema:about of	named entity 'potential' named entity 'determine' named entity 'real time' named entity 'African swine fever virus' named entity 'incorporates' named entity 'virus' named entity 'virus' named entity 'chimera' named entity 'virus' named entity 'living cells' named entity 'incorporation' named entity 'P54' named entity 'RECOMBINANT VIRUS' named entity 'CONFIRMED' named entity 'WILD-TYPE' named entity 'ENVELOPE PROTEIN' named entity 'VIRUS PARTICLE' named entity 'FORMATION' named entity 'APPLICATIONS' named entity 'LIVING' named entity 'PROTEIN ' named entity 'FULLY' named entity 'TRAJECTORY' named entity 'PARTICLES' named entity 'CONFOCAL MICROSCOPY' named entity 'TO TRACK' named entity 'FUNCTIONAL' named entity 'POSSIBLE' named entity 'CYCLE' named entity 'POTENTIAL' named entity 'PROTEIN FUSED' named entity 'FUSED TO' named entity 'REAL TIME' named entity 'PLAQUES' named entity 'ANIMATIONS' named entity 'INDIVIDUAL' named entity 'AFRICAN SWINE FEVER VIRUS' named entity 'MEMBRANE PROTEIN' named entity 'LIVING' named entity 'CELLS' named entity 'VIRUS INFECTION' named entity 'INFECTED' named entity 'DETERMINED' named entity 'PROTEIN ' named entity 'ANALYZED' named entity 'INTRACELLULAR' named entity 'GREEN FLUORESCENT PROTEIN' named entity 'P54' named entity 'VISUALIZATION' named entity 'CHIMERA' named entity 'VIRUS INFECTION' named entity 'RECOMBINANT' named entity 'PRESENTED' named entity 'EGFP' named entity 'STAGES' named entity 'FACTORY' named entity 'VIRUS PARTICLE' named entity 'DYNAMICS' named entity 'HAVE' named entity 'INFECTIOUS' named entity 'GROWTH' named entity 'CELLS' named entity 'FIRST STAGES' named entity 'PROGRESSION' named entity 'TYPE VIRUS' named entity 'TAGGED' named entity 'CHIMERA' named entity 'TIME' named entity 'DIFFERENT' named entity 'NORMAL' named entity 'USING' named entity 'ENHANCED GREEN FLUORESCENT PROTEIN' named entity 'DIVERSE' named entity 'VISUALIZED' named entity 'SPEED' named entity 'LIVE' named entity 'MOVEMENT' named entity 'FUSION PROTEIN' named entity 'STUDY'

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