About: Detection of transmissible gastroenteritis virus using cDNA probes

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About: Detection of transmissible gastroenteritis virus using cDNA probes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of transmissible gastroenteritis virus using cDNA probes
Creator	Bae, I Saif, L Wesley, R Benfield, D Jackwood, D
Source	Medline; PMC
abstract	Five cDNA probes prepared from molecular clones representing genomic RNA sequences of the virulent Miller strain of transmissible gastroenteritis virus (TGEV) were used in a dot blot hybridization assay to detect TGEV in cell culture and fecal specimens. Two clones (pA2 and pB4) represent nucleotide base pairs at the 3′ terminus of the Miller TGEV genome. The other three clones represent various portions of the 5′ end of the E 2 gene, which codes for the major surface glycoprotein of TGEV. Each of the(32)P-labeled cDNA probes hybridized to the virulent Miller, attenuated Purdue and four field strains of TGEV. The probes detected 200 to 2000 pg of TGEV RNA extracted from density gradient purified virions and did not hybridize RNA from mock-infected cell cultures, porcine rotavirus or antigenically unrelated coronaviruses. The pB4 and Hpa-1600 probes detected TGEV RNA sequences in 79 and 88%, respectively of 34 field samples identified as TGEV positive by the immunofluorescence assay and electron microscopy (EM). The pD24 clone, which is able to differentiate TGEV from the antigenically related coronaviruses, also compared favorably with conventional methods of EM and immunofluorescence for the detection of TGEV in fecal specimens.
has issue date	1991-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01319234
bibo:pmid	1848070
has license	no-cc
sha1sum (hex)	620da81618ad74da63a94c5e71368b0e54e2ca7b
schema:url	https://doi.org/10.1007/bf01319234
resource representing a document's title	Detection of transmissible gastroenteritis virus using cDNA probes
has PubMed Central identifier	PMC7086782
has PubMed identifier	1848070
schema:publication	Arch Virol
resource representing a document's body	covid:620da81618ad74da63a94c5e71368b0e54e2ca7b#body_text
is schema:about of	named entity 'pA2' named entity 'coronaviruses' named entity 'Each' named entity 'cell culture' named entity 'field' named entity 'detected' named entity 'Purdue' named entity 'codes' named entity 'sequences' named entity 'Detection' named entity 'INFECTED CELL' named entity 'METHODS' named entity 'PROBES' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'GENOME' named entity 'MILLER' named entity 'GENE' named entity 'RNA' named entity 'VARIOUS' named entity 'VIRULENT' named entity 'CORONAVIRUSES' named entity 'NUCLEOTIDE' named entity 'PREPARED' named entity 'CODES' covid:arg/620da81618ad74da63a94c5e71368b0e54e2ca7b named entity 'VIRIONS' named entity 'COMPARED' named entity 'USED' named entity 'LABELED' named entity 'REPRESENT' named entity 'PROBES' named entity 'DID' named entity 'EXTRACTED' named entity 'HPA' named entity 'PURIFIED' named entity '1600' named entity 'USING' named entity 'SAMPLES' named entity 'DETECTION' named entity 'MOLECULAR' named entity 'DETECTION' named entity 'CDNA' named entity 'IDENTIFIED' named entity 'MOCK' named entity 'DOT BLOT' named entity 'ELECTRON MICROSCOPY' named entity 'DIFFERENTIATE' named entity 'CONVENTIONAL' named entity '200' named entity 'IMMUNOFLUORESCENCE' named entity 'FECAL' named entity 'CDNA' named entity 'PORCINE ROTAVIRUS' named entity 'DENSITY GRADIENT' named entity 'CLONES' named entity 'TERMINUS' named entity 'BASE PAIRS' named entity 'HYBRIDIZATION ASSAY' named entity 'STRAIN' named entity 'FIELD' named entity 'END' named entity 'MAJOR' named entity 'GENOMIC' named entity 'DETECTED' named entity 'ATTENUATED' named entity 'UNRELATED' named entity 'CLONE' named entity '32P' named entity 'CELL CULTURES' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'RELATED' named entity '2000' named entity 'POSITIVE' named entity 'RNA SEQUENCES' named entity 'STRAINS' named entity 'TO DETECT' named entity 'SPECIMENS'

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