About: Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization

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About: Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
Creator	Kinoshita, Taroh Komazawa, Nobuyasu Kondoh, Gen Maeda, Yusuke Murata, Chie Nakatani, Yuka Okabe, Masaru Taguchi, Ryo Takeda, Junji Tojo, Hiromasa Yamagata, Kazuo
Source	PMC
abstract	The angiotensin-converting enzyme (ACE) is a key regulator of blood pressure. It is known to cleave small peptides, such as angiotensin I and bradykinin and changes their biological activities, leading to upregulation of blood pressure. Here we describe a new activity for ACE: a glycosylphosphatidylinositol (GPI)-anchored protein releasing activity (GPIase activity). Unlike its peptidase activity, GPIase activity is weakly inhibited by the tightly binding ACE inhibitor and not inactivated by substitutions of core amino acid residues for the peptidase activity, suggesting that the active site elements for GPIase differ from those for peptidase activity. ACE shed various GPI-anchored proteins from the cell surface, and the process was accelerated by the lipid raft disruptor filipin. The released products carried portions of the GPI anchor, indicating cleavage within the GPI moiety. Further analysis by high-performance liquid chromatography–mass spectrometry predicted the cleavage site at the mannose-mannose linkage. GPI-anchored proteins such as TESP5 and PH-20 were released from the sperm membrane of wild-type mice but not in Ace knockout sperm in vivo. Moreover, peptidase-inactivated E414D mutant ACE and also PI-PLC rescued the egg-binding deficiency of Ace knockout sperms, implying that ACE plays a crucial role in fertilization through this activity. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/nm1179) contains supplementary material, which is available to authorized users.
has issue date	2005-01-23(xsd:dateTime)
bibo:doi	10.1038/nm1179
bibo:pmid	15665832
has license	no-cc
sha1sum (hex)	624ca8416a345713fec250d38d71f24fac9905a2
schema:url	https://doi.org/10.1038/nm1179
resource representing a document's title	Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
has PubMed Central identifier	PMC7095966
has PubMed identifier	15665832
schema:publication	Nat Med
resource representing a document's body	covid:624ca8416a345713fec250d38d71f24fac9905a2#body_text
is schema:about of	named entity 'MEDICINE' named entity 'LEADING' named entity 'UPREGULATION OF BLOOD PRESSURE' named entity 'HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY' named entity 'IS A' named entity 'ACCELERATED' named entity 'BINDING' named entity 'ACE INHIBITOR' named entity 'DEFICIENCY OF' named entity 'ACTIVITY' named entity 'PI-PLC' named entity 'ANALYSIS' named entity 'ANGIOTENSIN I' named entity 'HERE' named entity 'PH-20' named entity 'CORE' named entity 'LINKAGE' named entity 'LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ' named entity 'VARIOUS' named entity 'BIOLOGICAL' named entity 'ROLE' named entity 'LIPID RAFT' named entity 'MOIETY' named entity 'CLEAVAGE' named entity 'NEW' named entity 'MICE' named entity 'MUTANT' named entity 'PLAYS' named entity 'PEPTIDASE ACTIVITY' named entity 'RELEASING' named entity 'WILD-TYPE' named entity 'KEY' named entity 'PROTEINS' named entity 'ELEMENTS' named entity 'BRADYKININ' named entity 'REGULATOR' named entity 'ITS' named entity 'FILIPIN' named entity 'INHIBITED' named entity 'PROTEIN ' named entity 'GPI' named entity 'PROCESS' named entity 'INDICATING' named entity 'CARRIED' named entity 'SPERM' named entity 'RELEASED' named entity 'PRODUCTS' named entity 'ACE' named entity 'PREDICTED' named entity 'BLOOD PRESSURE' named entity 'PEPTIDASE' named entity 'NUMBER' named entity 'ANCHORED' named entity 'THEIR' named entity 'GLYCOSYLPHOSPHATIDYLINOSITOL' named entity 'KNOWN' named entity 'CHANGES' named entity 'TESP5' named entity 'GPI ANCHOR' named entity 'TO CLEAVE' named entity 'PEPTIDES' named entity 'THE CELL' named entity 'FERTILIZATION' named entity 'ACTIVE SITE' named entity 'BUT' named entity 'SMALL' named entity 'SITE' named entity 'INACTIVATED' named entity 'ACTIVITIES' named entity 'AMINO ACID' named entity 'KNOCKOUT'

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