About: Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies

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About: Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies
Creator	Wang, Jianwei Huang, Cheng Boeren, Sjef Abd-Alla, Adly Agahince, İkbal Kariithi, Henry Meki, Irene Murungi, Edwin Nyanjom, Steven Otieno, Everlyne Van Oers, Monique Vlak, Just
Source	PMC
abstract	Glossina pallidipes salivary gland hypertrophy virus (GpSGHV; family Hytrosaviridae) is a dsDNA virus exclusively pathogenic to tsetse flies (Diptera; Glossinidae). The 190 kb GpSGHV genome contains 160 open reading frames and encodes more than 60 confirmed proteins. The asymptomatic GpSGHV infection in flies can convert to symptomatic infection that is characterized by overt salivary gland hypertrophy (SGH). Flies with SGH show reduced general fitness and reproductive dysfunction. Although the occurrence of SGH is an exception rather than the rule, G. pallidipes is thought to be the most susceptible to expression of overt SGH symptoms compared to other Glossina species that are largely asymptomatic. Although Glossina salivary glands (SGs) play an essential role in GpSGHV transmission, the functions of the salivary components during the virus infection are poorly understood. In this study, we used mass spectrometry to study SG proteomes of G. pallidipes and G. m. morsitans, two Glossina model species that exhibit differential GpSGHV pathologies (high and low incidence of SGH, respectively). A total of 540 host proteins were identified, of which 23 and 9 proteins were significantly up- and down-regulated, respectively, in G. pallidipes compared to G. m. morsitans. Whereas 58 GpSGHV proteins were detected in G. pallidipes F(1) progenies, only 5 viral proteins were detected in G. m. morsitans. Unlike in G. pallidipes, qPCR assay did not show any significant increase in virus titers in G. m. morsitans F(1) progenies, confirming that G. m. morsitans is less susceptible to GpSGHV infection and replication compared to G. pallidipes. Based on our results, we speculate that in the case of G. pallidipes, GpSGHV employs a repertoire of host intracellular signaling pathways for successful infection. In the case of G. m. morsitans, antiviral responses appeared to be dominant. These results are useful for designing additional tools to investigate the Glossina-GpSGHV interactions.
has issue date	2016-02-09(xsd:dateTime)
bibo:doi	10.3389/fmicb.2016.00089
bibo:pmid	26903969
has license	cc-by
sha1sum (hex)	62a1f28a0ad0c2c37252351ad64fd7d8b0efdcc4
schema:url	https://doi.org/10.3389/fmicb.2016.00089
resource representing a document's title	Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies
has PubMed Central identifier	PMC4746320
has PubMed identifier	26903969
schema:publication	Front Microbiol
resource representing a document's body	covid:62a1f28a0ad0c2c37252351ad64fd7d8b0efdcc4#body_text
is schema:about of	named entity 'MICROBIOLOGY' named entity 'SPECIES' named entity 'SECTION' named entity 'JOURNAL ' named entity 'EXHIBIT' named entity 'SALIVARY GLAND' named entity '80%' named entity 'A.M.' named entity 'AUTHORS' named entity 'PATHOLOGIES' named entity 'DIFFERENTIAL' named entity 'PROTEOMES' named entity 'COMPARATIVE ANALYSIS' named entity 'THESE' named entity 'ABD' named entity 'ORG' named entity 'ALLA' named entity 'WORK' named entity 'HAVE' named entity 'GLOSSINA' covid:arg/62a1f28a0ad0c2c37252351ad64fd7d8b0efdcc4 named entity 'HIV-1' named entity 'V-ATPase' named entity 'flies' named entity 'β-tubulin' named entity 'bacteriocytes' named entity 'membrane trafficking' named entity 'G. pallidipes' named entity 'hemocoel' named entity 'HSV-1' named entity 'molecular marker' named entity 'progenies' named entity 'SDS-PAGE' named entity 'G. pallidipes' named entity 'tsetse fly' named entity 'viruses' named entity 'G. pallidipes' named entity 'salivary gland' named entity 'Tryptic' named entity 'virus infections' named entity 'progenies' named entity 'flies' named entity 'virus infection' named entity 'Glossina' named entity 'bioinformatics' named entity 'infection' named entity 'protein expression' named entity 'viral envelope' named entity 'enveloped viruses' named entity 'Harrist' named entity 'pupae' named entity 'pathogenesis' named entity 'pathogenesis' named entity 'intracellular trafficking' named entity 'virus replication' named entity 'tsetse fly' named entity 'G. pallidipes' named entity 'viral pathogenesis' named entity 'integrin' named entity 'Insect Pest' named entity 'glycoprotein' named entity 'HCV' named entity 'genome' named entity 'protein' named entity 'Wigglesworthia glossinidia' named entity 'G. pallidipes' named entity 'mass spectrometry' named entity 'homogenizer' named entity 'glycolytic' named entity 'virus infections'

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