About: Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells
Creator	Ding, Xiuyan Tao, Jie Wang, Jianye Zhao, Xin Zhu, Guoqiang Zhu, Liqian
Source	Elsevier; Medline; PMC
abstract	Cholesterol is involved in the life cycle of many viruses. Here, we examined the role of cholesterol for both viral envelope and target cell membrane for bovine herpesvirus type 1 (BoHV-1) infection. Cholesterol depletion by pretreatment of Madin–Darby bovine kidney (MDBK) cells with a cholesterol-sequestering drug methyl-β-cyclodextrin (MβCD), inhibited the production of BoHV-1 in a dose-dependent manner. This inhibitory effect was partially reversed by cholesterol replenishment, indicating that the reduction was caused by cholesterol depletion. Cholesterol depletion at the post-entry stage only had a mild effect on the virus production. However, cell membrane cholesterol depletion did not reduce the virus attachment. In addition, treatment of BoHV-1 particles with MβCD also reduced the virus infectivity significantly and the effect was partially reversed by addition of exogenous cholesterol. Taken together, these data implicated that cell membrane cholesterol mainly contributed to BoHV-1 entry into MDBK cells and the viral envelope cholesterol was also essential for the virus infectivity.
has issue date	2010-07-29(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2009.12.031
bibo:pmid	20097021
has license	no-cc
sha1sum (hex)	62d8d4b899c21625ecd7decbed3d3a548a23ed1d
schema:url	https://doi.org/10.1016/j.vetmic.2009.12.031
resource representing a document's title	Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells
has PubMed Central identifier	PMC7117431
has PubMed identifier	20097021
schema:publication	Vet Microbiol
resource representing a document's body	covid:62d8d4b899c21625ecd7decbed3d3a548a23ed1d#body_text
is schema:about of	named entity 'ROLE' named entity 'CRITICAL' named entity 'MDBK' named entity 'INFECTION' named entity 'BOVINE HERPESVIRUS TYPE 1' named entity 'CHOLESTEROL' named entity 'CELLS' named entity 'Critical role' named entity 'herpesvirus' named entity 'infection' named entity 'cholera toxin' named entity 'Lipid rafts' named entity 'bovine' named entity 'virus' named entity 'infection' named entity 'CTB' named entity 'virus' named entity 'herpesvirus' named entity 'Biotechnology' named entity 'virus' named entity 'Semliki Forest virus' named entity 'Cholesterol depletion' named entity 'biological process' named entity 'DMEM' named entity 'cholesterol' named entity 'kidney' named entity 'dose-dependent manner' named entity 'receptor' named entity 'virus' named entity 'cholesterol' named entity 'unsaturated' named entity 'ultracentrifugation' named entity 'cholesterol' named entity 'plaque formation' named entity 'virus' named entity 'virion particles' named entity 'culture medium' named entity 'virus' named entity 'reagent' named entity 'virus' named entity 'infectivity' named entity 'cholesterol' named entity 'ganglioside' named entity 'DMEM' named entity 'virus entry' named entity 'cholesterol' named entity 'lipid rafts' named entity 'cell membrane' named entity 'FACS' named entity 'PBS' named entity 'virus' named entity 'cholesterol depletion' named entity 'Biosynthesis' named entity 'lipid rafts' named entity 'HSV-1' named entity 'varicella-zoster virus' named entity 'HIV-1' named entity 'bovine' named entity 'virions' named entity 'pathogens' named entity 'endosomal membrane' named entity 'cholesterol' named entity 'virus entry' named entity 'cholesterol' named entity 'cholesterol depletion' named entity 'homolog' named entity 'optical density' named entity 'kDa' named entity 'biological processes' named entity 'virus' named entity 'viruses' named entity 'titrated' named entity 'lipid rafts' named entity 'Cholesterol' named entity 'room temperature' named entity 'virus'

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