About: Multiplexed Nucleic Acid Programmable Protein Arrays

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multiplexed Nucleic Acid Programmable Protein Arrays
Creator	Li, Han Liu, Lei Wang, Jie Bian, Xiaofang Bui, Hoang Duan, Hu Magee, Mitch Park, Jin Petritis, Brianne Qiu, Ji Song, Lusheng Viloria, Jennifer Wang, Haoyu Yang, Liuhui Yu, Xiaobo Achkar, Jacqueline Labaer, Joshua Mcmurray, David
source	PMC
abstract	Rationale: Cell-free protein microarrays display naturally-folded proteins based on just-in-time in situ synthesis, and have made important contributions to basic and translational research. However, the risk of spot-to-spot cross-talk from protein diffusion during expression has limited the feature density of these arrays. Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot. Results: Even when proteins of different sizes were displayed within the same feature, they were readily detected using protein-specific antibodies. Protein-protein interactions and serological antibody assays using human viral proteome microarrays demonstrated that comparable hits were detected by M-NAPPA and non-multiplexed NAPPA arrays. An ultra-high density proteome microarray displaying > 16k proteins on a single microscope slide was produced by combining M-NAPPA with a photolithography-based silicon nano-well platform. Finally, four new tuberculosis-related antigens in guinea pigs vaccinated with Bacillus Calmette-Guerin (BCG) were identified with M-NAPPA and validated with ELISA. Conclusion: All data demonstrate that multiplexing features on a protein microarray offer a cost-effective fabrication approach and have the potential to facilitate high throughput translational research.
has issue date	2017-09-20(xsd:dateTime)
bibo:doi	10.7150/thno.20151
bibo:pmid	29109798
has license	cc-by-nc
sha1sum (hex)	636a2debbb56c9142bbf960d9a200665c556e05a
schema:url	https://doi.org/10.7150/thno.20151
resource representing a document's title	Multiplexed Nucleic Acid Programmable Protein Arrays
has PubMed Central identifier	PMC5667425
has PubMed identifier	29109798
schema:publication	Theranostics
resource representing a document's body	covid:636a2debbb56c9142bbf960d9a200665c556e05a#body_text
is schema:about of	named entity 'NUCLEIC ACID' named entity 'PROTEIN ARRAYS' named entity 'protein microarrays' named entity 'translational research' named entity 'antibody' named entity 'cell wall' named entity 'centrifugation' named entity 'brain tissue' named entity 'GAD2' named entity 'antibody' named entity 'Alexa Fluor' named entity 'Protein microarrays' named entity 'protein' named entity 'multiplexed' named entity 'protein microarrays' named entity 'proteome' named entity 'BCG' named entity 'Figure 8' named entity 'antibodies' named entity 'Tecan' named entity 'University of Florida' named entity 'plasmids' named entity 'false positives' named entity 'protein families' named entity 'mass spectrometry' named entity 'multiplexed' named entity 'mycobacterial' named entity 'polyclonal' named entity 'fluorescent' named entity 'multiplexed' named entity 'type 1 diabetes' named entity 'microscope slide' named entity 'protein-protein interactions' named entity 'fluorophore' named entity 'protein expression' named entity 'multiplexed' named entity 'protein' named entity 'antibodies' named entity 'secondary antibody' named entity 'protein' named entity 'Bronx' named entity 'Purifying proteins' named entity 'mathematical model' named entity 'multiple sclerosis' named entity 'plasmids' named entity 'lysate' named entity 'p-value' named entity 'microarrays' named entity 'biomarker' named entity 'proteome' named entity 'antibody reactivity' named entity 'Bacillus Calmette-Guerin' named entity 'wheat germ' named entity 'Clusterin' named entity 'multiplexed' named entity 'plasmids' named entity 'transcription' named entity 'LidA' named entity 'transcriptional' named entity 'mathematical model' named entity 'microarray' named entity 'Männedorf' named entity 'Thermo Fisher Scientific' named entity 'HLA'

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