About: Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine

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About: Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine
Creator	Zhou, Ling Lan, Tian Sun, Yuan Ma, Jingyun Ma, J Liu, Y Li, Qianniu Zhou, L Chen, Y Li, Q Lu, X Sun, Y Lan, T Fang, X Chen, Yonghui Du, Mengkan Fang, Xueen Liu, Yanhong Lu, Xiandong Du, M Xiandong,
Source	Elsevier; Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome-coronavirus (SADS-CoV) are three emerging and re-emerging coronaviruses (CoVs). Symptoms caused by these three viruses are extremely similar, including acute diarrhea, vomiting and even death in piglets. To date, strict biosecurity is still the most effective disease prevention and control measures, and the early detection of pathogens is the most important link. Here, we developed a microfluidic-RT-LAMP chip detection system for the first time, which could detected PEDV, PDCoV and SADS-CoV simultaneously, and had advantages of rapid, simple, sensitive, high-throughput, and accurate at point-of-care settings. The lowest detection limits of the microfluidic-RT-LAMP chip method are 10(1) copies/μL, 10(2) copies/μL and 10(2) copies/μL for PEDV, PDCoV and SADS-CoV, respectively. The whole detection procedure can be finished rapidly in 40 min without any cross-reaction with other common swine viruses. A total of 173 clinical swine fecal samples characterized with diarrheal symptoms were used to evaluate the performance of the newly developed system, which presented good stabilities (C.V.s<5%) and specificities (100%), and possessed sensitivities of 92.24%, 92.19% and 91.23% for PEDV, PDCoV and SADS-CoV respectively. In summary, the established microfluidic-RT-LAMP chip detection system could satisfy the demanding in field diagnoses, which was suitable for promotion in remote areas due to its fast, portable and cost-effective characters.
has issue date	2020-05-19(xsd:dateTime)
bibo:doi	10.1016/j.aca.2020.05.034
bibo:pmid	32674781
has license	no-cc
sha1sum (hex)	64570c31c33d1f472894e24bab32f683ff3301c1
schema:url	https://doi.org/10.1016/j.aca.2020.05.034
resource representing a document's title	Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine
has PubMed Central identifier	PMC7234951
has PubMed identifier	32674781
schema:publication	Anal Chim Acta
resource representing a document's body	covid:64570c31c33d1f472894e24bab32f683ff3301c1#body_text
is schema:about of	named entity 'detection' named entity 'copies' named entity 'detection' named entity 'control' named entity 'promotion' named entity 'Symptoms' named entity 'https' named entity 'enteric' named entity 'emerging' named entity 'coronaviruses' named entity 'chip' named entity 'emerging' named entity 'cross-reaction' named entity 'acute' named entity 'porcine' named entity 'diarrhea' named entity 'rapidly' named entity 'swine' named entity 'possessed' named entity 'biosecurity' named entity 'fecal' named entity 'vomiting' named entity 'accurate' named entity 'Journal' named entity 'diarrhea' named entity 'biosecurity' named entity 'PEDV' named entity 'viruses' named entity 'PEDV' named entity 'Microfluidic' named entity 'coronaviruses' named entity 'porcine' named entity 'Porcine epidemic diarrhea virus' named entity 'coronaviruses' named entity 'Microfluidic' named entity 'Microfluidic' named entity 'point-of-care' named entity 'pathogens' named entity '2.5 mm' named entity 'microfluidic chip' named entity 'ball valve' named entity 'microfluidic chip' named entity 'injection molding' named entity 'laboratory diagnosis' named entity 'early diagnosis' named entity 'immunohistochemistry' named entity 'PCR' named entity 'enzyme-linked immunosorbent assay' named entity 'microfluidic' named entity 'diarrhea' named entity 'microfluidic chip' named entity 'vomiting' named entity 'diagnostic assays' named entity 'microfluidic' named entity 'agarose gel electrophoresis' named entity 'pathogens' named entity 'microfluidic' named entity 'PMMA' named entity 'pathogens' named entity 'agarose gel electrophoresis' named entity 'Diarrhea'

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