About: A Universal Influenza A and B Duplex Real‐time RT‐PCR Assay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Universal Influenza A and B Duplex Real‐time RT‐PCR Assay
Creator	Chiu, Lily Koay, Lee, Chun Lee, Hong Siew-Chuan, Evelyn Loh, Tang, Wei-Tze Tze, Ping
Source	Medline; PMC
abstract	A high throughput universal influenza A and B duplex real‐time RT‐PCR was developed to meet effectively the heightened surveillance and diagnostic needs essential in managing influenza infections and outbreaks. Primers and probes, designed to target highly conserved regions of the matrix protein of influenza A and the nucleoprotein of influenza B, were optimized using the high‐throughput LightCycler 480 II system. Analytical sensitivity and specificity were characterized using RNA transcripts diluted serially, archived non‐influenza respiratory viruses, and proficiency test samples. Eighty‐nine clinical samples were tested in parallel against existing influenza A and B monoplex assays. Once validated, the duplex assay was applied prospectively on 2,458 clinical specimens that were later subtyped. In April 2011, the emergence of an influenza B variant necessitated the inclusion of an additional modified probe for influenza B and revalidation of the revised protocol. The lower detection limits of the assay were 50 copies/PCR. There was no cross‐reactivity against any non‐influenza respiratory virus and all proficiency testing materials were identified correctly. The parallel testing revealed a 98.9% overall agreement. Routine application of the assay revealed high sensitivity and specificity for the detection of influenza A/H1N1/2009, A/H3N2 and influenza B. Assay C(q) values correlated well between the pre‐ and post‐revision protocols for influenza A (r(2) = 0.998) and B (r(2) = 0.999). The revised protocol detected three additional novel influenza B variant cases in 200 specimens reported previously as influenza B negative. This in‐house assay offers a highly sensitive and specific option for laboratories seeking to expand their influenza testing capacity. J. Med. Virol. 84:1646–1651, 2012. © 2012 Wiley Periodicals, Inc.
has issue date	2012-08-15(xsd:dateTime)
bibo:doi	10.1002/jmv.23375
bibo:pmid	22930514
has license	no-cc
sha1sum (hex)	652cb171b207e44d6155fb221644297f23ed73df
schema:url	https://doi.org/10.1002/jmv.23375
resource representing a document's title	A Universal Influenza A and B Duplex Real‐time RT‐PCR Assay
has PubMed Central identifier	PMC7166972
has PubMed identifier	22930514
schema:publication	J Med Virol
resource representing a document's body	covid:652cb171b207e44d6155fb221644297f23ed73df#body_text
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