About: A cell-based, quantitative and isoform-specific assay for exchange proteins directly activated by cAMP

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A cell-based, quantitative and isoform-specific assay for exchange proteins directly activated by cAMP
Creator	Mei, Fang Zhu, Yingmin Luo, Pei Cheng, &
source	PMC
abstract	Extensive functional studies of the exchange protein directly activated by cAMP (EPAC) family of signaling molecules have demonstrated that EPAC proteins play a fundamental role in several physiological and pathophysiological responses, therefore are attractive drug targets. In this report, the development of a cell-based, medium to high throughput screening assay that is capable of monitoring EPAC-mediated activation of cellular Rap1 in an isoform-specific manner is described. This assay adapts a conventional ELISA format with immobilized RalGDS-RBD as a bait to selectively capture GTP-bound active Rap1. As a result, it fills an urgent need for a cell-based EPAC assay that can be conveniently performed using microtiter plates for the discovery and/or validation of isoform-specific EPAC agonists and antagonists.
has issue date	2017-07-24(xsd:dateTime)
bibo:doi	10.1038/s41598-017-06432-4
bibo:pmid	28740152
has license	cc-by
sha1sum (hex)	6534f8468fbb6dbdaf078a9a59d92dc686a2c4b1
schema:url	https://doi.org/10.1038/s41598-017-06432-4
resource representing a document's title	A cell-based, quantitative and isoform-specific assay for exchange proteins directly activated by cAMP
has PubMed Central identifier	PMC5524698
has PubMed identifier	28740152
schema:publication	Sci Rep
resource representing a document's body	covid:6534f8468fbb6dbdaf078a9a59d92dc686a2c4b1#body_text
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