About: Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41
Creator	Matsuoka, Masao Fujii, Nobutaka Nishikawa, Hiroki Kodama, Eiichi Fukudome, Ayako Izumi, Kazuki Oishi, Shinya Sakakibara, Ayako
Source	Elsevier; Medline; PMC
abstract	Abstract Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents that block HIV-1 fusion, two screening methods based on detection and quantification by the enzyme-linked immunosorbent assay (ELISA) principle have been established. One method uses an alkaline phosphatase (ALP)-conjugated antibody (Ab-ELISA) and the other uses an ALP-fused HR (F-ELISA) to detect and quantify the interaction of the two HRs. The F-ELISA was more simple and rapid, since no ALP-conjugated antibody reaction was required. Both ELISAs detected all the fusion inhibitors tested except for T-20. Interaction of the two HRs was observed in both ELISAs, even in the presence of 10% dimethyl sulfoxide. Ab-ELISA performed best in a pH ranging from 6 to 8, while F-ELISA performed best at a pH ranging from 7 to 8. These results indicate that both established ELISAs are suitable for the identification of HIV-1 fusion inhibitors.
has issue date	2008-10-31(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2008.05.006
bibo:pmid	18584890
has license	els-covid
sha1sum (hex)	6563d23edf1e04da084f6432e791dc7fc9c48a51
schema:url	https://doi.org/10.1016/j.antiviral.2008.05.006
resource representing a document's title	Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41
has PubMed Central identifier	PMC7114109
has PubMed identifier	18584890
schema:publication	Antiviral Research
resource representing a document's body	covid:6563d23edf1e04da084f6432e791dc7fc9c48a51#body_text
is schema:about of	named entity 'SYSTEMS' named entity 'inhibitors' named entity 'HIV-1' named entity 'EXTRA' named entity 'RAPID' named entity 'SUITABLE' named entity 'ELISA' named entity 'PLAYS' named entity 'ANTIBODY' named entity 'EXTRA' named entity 'DETECTED' named entity 'VIRION' named entity 'MEDIATED' named entity 'GP41' named entity 'BASED' named entity 'ENFUVIRTIDE' named entity '10%' named entity 'PRINCIPLE' named entity 'SCREENING' named entity 'HIV-1' named entity 'REPEATS' named entity 'BEST' named entity 'ALKALINE PHOSPHATASE ' named entity 'ITS' named entity 'PRESENCE OF' named entity 'ROLE' named entity 'ALP' named entity 'TO IDENTIFY' named entity 'ENTRY' named entity 'METHOD' named entity 'USES' named entity 'SCREENING' named entity 'MEDIATED' named entity 'GP41' named entity 'FUSION' named entity 'NOVEL' named entity 'OBSERVED' named entity 'EXCEPT FOR' named entity 'DIMETHYL SULFOXIDE' named entity 'HAVE' named entity 'ENVELOPE PROTEIN' named entity 'DETECTION' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'HRS' named entity 'CONJUGATED' named entity 'VIRION' named entity 'HUMAN IMMUNODEFICIENCY VIRUS TYPE 1' named entity 'PERFORMED' named entity 'HIV-1' named entity 'THESE' named entity 'FUSION' named entity '20%' named entity 'BLOCK' named entity 'QUANTIFICATION' named entity 'REACTION' named entity 'TESTED' named entity 'ESTABLISHED' named entity 'INHIBITOR' named entity 'TARGET CELLS' named entity 'SIMPLE' named entity 'PROTEIN ' named entity 'RESULTS' named entity 'BLOCKS' named entity 'TO DETECT' named entity 'FUSION INHIBITORS' named entity 'METHODS' named entity 'FUSED' named entity 'REQUIRED' named entity 'MEMBRANE FUSION' named entity 'IDENTIFICATION' named entity 'REPEATS' named entity 'INTERACTION' covid:arg/6563d23edf1e04da084f6432e791dc7fc9c48a51 named entity 'conjugated'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software