About: Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins

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About: Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins
Creator	Inada, T Kameoka, Y Matsushita, S Omoe, K Sakaguchi, M Takahashi-Omoe, H
Source	Elsevier; Medline; PMC
abstract	Abstract Lactate dehydrogenase-elevating virus (LDV) has a strict species-specificity. Because only a subset of mouse primary macrophages have been identified that can support LDV replication in vitro, the precise molecular mechanism of viral entry and replication remains unclear. To analyze the LDV envelope proteins, which probably mediate viral attachment to the host cell, we developed a mammalian system for stable co-expression of LDV open reading frame (ORF) 5- and ORF 6-encoded proteins (ORF 5 and ORF 6 proteins), which correspond to envelope VP-3 and M/VP-2, respectively, and compared these expressed proteins to the native ones. Western blotting analysis combined with N-glycanase digestion revealed that ORF 5 and ORF 6 proteins were similar in size to native VP-3 and M/VP-2, and that ORF 5 protein was N-glycosylated, like the native VP-3. Immunofluorescence microscopy revealed that both ORF 5 and ORF 6 proteins were distributed throughout the cytoplasm and were colocalized in most cells. Moreover, ORF 5 protein was localized both in the perinuclear region and the Golgi complex and transported to the cell surface. This mammalian expression system in which the exogenously expressed proteins closely resemble the native proteins will provide the experimental basis for further studies of the interactions between LDV envelope proteins and host cells.
has issue date	2004-03-31(xsd:dateTime)
bibo:doi	10.1016/s0147-9571(03)00053-5
bibo:pmid	14690718
has license	els-covid
sha1sum (hex)	657602c5e4a96575433b73793bbf7e33110262e7
schema:url	https://doi.org/10.1016/s0147-9571%2803%2900053-5
resource representing a document's title	Analysis of protein expression by mammalian cell lines stably expressing lactate dehydrogenase-elevating virus ORF 5 and ORF 6 proteins
has PubMed Central identifier	PMC7124688
has PubMed identifier	14690718
schema:publication	Comparative Immunology, Microbiology and Infectious Diseases
resource representing a document's body	covid:657602c5e4a96575433b73793bbf7e33110262e7#body_text
is schema:about of	named entity 'MAMMALIAN CELL' named entity 'PROTEINS' named entity 'CELL LINES' named entity 'macrophages' named entity 'subset' named entity 'ORF' named entity 'expressing' named entity 'virus' named entity 'protein' named entity 'cell lines' named entity 'VIRUS' named entity 'ORF' named entity 'HOST CELL' named entity 'IDENTIFIED' named entity 'STUDIES' named entity 'CELLS' named entity 'SUPPORT' named entity 'ANALYSIS' named entity 'PROTEIN ' named entity 'VIRAL ENTRY' named entity 'EXPRESSED' named entity 'LIKE' named entity 'VIRAL ATTACHMENT' named entity 'HOST CELLS' named entity 'MAMMALIAN' named entity 'MACROPHAGES' named entity 'IN VITRO' named entity 'COMBINED' named entity 'OPEN READING FRAME' named entity 'PROTEIN EXPRESSION' named entity 'CO-EXPRESSION' named entity 'LACTATE DEHYDROGENASE-ELEVATING VIRUS' named entity 'INTERACTIONS' named entity 'SYSTEM' named entity 'ELEVATING' named entity 'ANALYSIS' named entity 'PROBABLY' named entity 'SIMILAR' named entity 'DISTRIBUTED' named entity 'SUBSET' named entity 'REVEALED' named entity 'ORF' named entity 'SPECIES' named entity 'SPECIFICITY' named entity 'PROTEINS' named entity 'CYTOPLASM' named entity 'PRIMARY' named entity 'GOLGI COMPLEX' named entity 'LACTATE DEHYDROGENASE' named entity 'CELL SURFACE' named entity 'BASIS' named entity 'GLYCOSYLATED' named entity 'ENCODED' named entity 'SIZE' named entity 'EXPRESSION SYSTEM' named entity 'ENVELOPE' named entity 'COMPARED' named entity 'HAVE' named entity 'LOCALIZED' named entity 'REGION' named entity 'STABLE' named entity 'PERINUCLEAR' named entity 'MOLECULAR MECHANISM' named entity 'MEDIATE' named entity 'NATIVE' named entity 'PRECISE' named entity 'EXPERIMENTAL' named entity 'PROVIDE' named entity 'THESE' named entity 'IMMUNOFLUORESCENCE MICROSCOPY' named entity 'MOUSE' named entity 'UNCLEAR' named entity 'DIGESTION' named entity 'REPLICATION' named entity 'WESTERN BLOTTING' covid:arg/657602c5e4a96575433b73793bbf7e33110262e7

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