About: Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein

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About: Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein
Creator	Chen, Wei Wang, Yue-Dan
Source	Elsevier; Medline; PMC
abstract	To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2(+) SARS-recovered donors at different time points post disease. CD8(+)DimerX-S1203(+) CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8(+)DimerX-S1203(+) cells paralleled the numbers of interferon-γ-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus.
has issue date	2004-09-11(xsd:dateTime)
bibo:doi	10.1016/j.clim.2004.07.004
bibo:pmid	15451471
has license	no-cc
sha1sum (hex)	6619829d93f0e2761fa149b5ae8d5a77ca13adbc
schema:url	https://doi.org/10.1016/j.clim.2004.07.004
resource representing a document's title	Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein
has PubMed Central identifier	PMC7106176
has PubMed identifier	15451471
schema:publication	Clin Immunol
resource representing a document's body	covid:6619829d93f0e2761fa149b5ae8d5a77ca13adbc#body_text
is schema:about of	named entity 'Severe acute respiratory syndrome (SARS)' named entity 'CYTOTOXIC' named entity 'SPECIFIC' named entity 'HLA-A2' named entity 'UP TO' named entity 'DIFFERENT' named entity 'NEWLY' named entity 'IDENTIFIED' named entity 'NUMBERS' named entity 'CONCLUSION' covid:arg/6619829d93f0e2761fa149b5ae8d5a77ca13adbc named entity 'ELISPOT ASSAY' named entity 'ASSESS' named entity 'FUSION PROTEIN' named entity 'POSITIVE' named entity 'USING' named entity 'SCREEN' named entity 'POINTS' named entity 'SARS' named entity 'DETECTING' named entity 'HLA-A2' named entity 'SPECIFIC' named entity 'FLOW CYTOMETRY ASSAY' named entity 'MODIFIED' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELL' named entity 'FLOW CYTOMETRY' named entity 'THESE' named entity 'CTL' named entity 'INTERFERON' named entity '3 MONTHS' named entity 'T LYMPHOCYTES' named entity 'REAL-TIME' named entity 'CORONAVIRUS' named entity 'T LYMPHOCYTES' named entity 'POST' named entity 'CYTOTOXIC' named entity 'BASED' named entity 'EPITOPES' named entity 'SPOTS' named entity 'DONORS' named entity 'CELLS' named entity 'DIRECTED' named entity 'SARS' named entity 'RECOVERED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'PEPTIDE' named entity 'METHOD' named entity 'RECOVERY' named entity 'PERFORMED' named entity 'CORONAVIRUS' named entity 'CD8' named entity 'VIRUS' named entity 'STAINING' named entity 'DISEASE' named entity 'TIME' named entity 'DETECTED' named entity 'PBMC' named entity 'staining' named entity 'flow cytometry' named entity 'flow cytometry' named entity 'time' named entity 'PBMC' named entity 'SARS' named entity 'epitopes' named entity 'flow cytometry' named entity 'HLA-A2' named entity 'coronavirus' named entity 'staining' named entity 'CTL' named entity 'assay' named entity 'fusion protein' named entity 'CD8 +' named entity 'cytotoxic T lymphocytes' named entity 'peripheral blood mononuclear cell' named entity 'Severe acute respiratory syndrome' named entity 'interferon' named entity 'SARS' named entity 'PBMC' named entity 'coronavirus' named entity 'donors'

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