About: Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA

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About: Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA
Creator	Allende, Maria Jaskiewicz, Ewa Young, William
source	PMC
abstract	Brefeldin A reversibly disassembles the Golgi complex, causing mixing of the Golgi cisternae with the ER while the trans Golgi network persists as part of a separate endosomal membrane system. Because of this compartmental separation, Brefeldin A treatment has been used to map the sub-Golgi locations of several Golgi enzymes including GM2 synthase. We previously proposed that GM2 synthase might be located in a distal portion of the Golgi complex which in the presence of Brefeldin A would be separated from the substrate ganglioside GM3 present in the mixed ER-Golgi membrane system. In the present study we show using GM2 synthase chimeras that GM2 synthesis was blocked by Brefeldin A when GM2 synthase was distributed throughout all Golgi subcompartments or even when it was restricted to the medial Golgi. Because these findings opposed our speculation regarding a distal location of this enzyme, we sought an alternative explanation for the inhibition of ganglioside synthesis by Brefeldin A. However, Brefeldin A did not degrade GM2 synthase, prevent its homodimerization, or inhibit its in vitro activity. Brefeldin A did result in the conversion of a portion of membrane bound GM2 synthase into a soluble form which has minimal capability to produce GM2 in whole cells. However, this conversion was not sufficient to explain the nearly total loss of GM2 production in intact cells in the presence of Brefeldin A. Nevertheless, the results of this study indicate that Brefeldin A-induced inhibition of ganglioside synthesis cannot be used to deduce the location of GM2 synthase.
has issue date	1999-07-01(xsd:dateTime)
bibo:doi	10.1093/glycob/9.7.689
bibo:pmid	10362838
has license	no-cc
sha1sum (hex)	662df200b97c40499541945ec265a98a84820e88
schema:url	https://doi.org/10.1093/glycob/9.7.689
resource representing a document's title	Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA
has PubMed Central identifier	PMC7108590
has PubMed identifier	10362838
schema:publication	Glycobiology
resource representing a document's body	covid:662df200b97c40499541945ec265a98a84820e88#body_text
is schema:about of	named entity 'GM2' named entity 'synthase' named entity 'inhibition' named entity 'presence' named entity 'Brefeldin A' named entity 'DISTRIBUTED' named entity 'PRESENT' named entity 'BLOCKED' named entity 'IN VITRO ACTIVITY' named entity 'ENZYMES' named entity 'STUDY' named entity 'LOCATIONS' named entity 'INDUCED' named entity 'BOUND' named entity 'ENDOSOMAL MEMBRANE' named entity 'ALTERNATIVE' named entity 'SUB-' named entity 'PREVENT' named entity 'USED' named entity 'PROPOSED' named entity 'USING' named entity 'CELLS' named entity 'RESTRICTED' named entity 'SYNTHESIS' covid:arg/662df200b97c40499541945ec265a98a84820e88 named entity 'SYNTHESIS' named entity 'NEARLY' named entity 'PORTION' named entity 'GOLGI MEMBRANE' named entity 'TO PRODUCE' named entity 'MEMBRANE' named entity 'SEPARATED FROM' named entity 'CAUSING' named entity 'GANGLIOSIDE GM3' named entity 'INTACT' named entity 'LOSS OF' named entity 'DISTAL' named entity 'CONVERSION' named entity 'EXPLANATION' named entity 'TRANS GOLGI NETWORK' named entity 'GANGLIOSIDE SYNTHESIS' named entity 'SUFFICIENT' named entity 'SPECULATION' named entity 'PRODUCTION' named entity 'PART OF' named entity 'INCLUDING' named entity 'BREFELDIN A' named entity 'TREATMENT' named entity 'MEMBRANE SYSTEM' named entity 'MIGHT BE' named entity 'MIXED' named entity 'PREVIOUSLY' named entity 'TOTAL' named entity 'RESULT' named entity 'GM2' named entity 'INHIBITION' named entity 'ER-' named entity 'EFFECT' named entity 'BFA' named entity 'GANGLIOSIDE SYNTHESIS' named entity 'LOCATION' named entity 'GM2 SYNTHASE' named entity 'GM2' named entity 'ITS' named entity 'MINIMAL' named entity 'GOLGI COMPLEX' named entity 'CAPABILITY' named entity 'SUBSTRATE' named entity 'MAP' named entity 'LOCATION' named entity 'BREFELDIN A' named entity 'HOMODIMERIZATION' named entity 'GOLGI' named entity 'PRESENCE OF' named entity 'MIXING' named entity 'SOLUBLE FORM' named entity 'LOCATED IN' named entity 'FINDINGS' named entity 'GM2 SYNTHASE'

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