About: Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
Creator	Millet, Jean Whittaker, Gary
source	Medline; PMC
abstract	The coronavirus spike envelope glycoprotein is an essential viral component that mediates virus entry events. Biochemical assessment of the spike protein is critical for understanding structure–function relationships and the roles of the protein in the viral life cycle. Coronavirus spike proteins are typically proteolytically processed and activated by host cell enzymes such as trypsin-like proteases, cathepsins, or proprotein-convertases. Analysis of coronavirus spike proteins by western blot allows the visualization and assessment of proteolytic processing by endogenous or exogenous proteases. Here, we present a method based on western blot analysis to investigate spike protein proteolytic cleavage by transient transfection of HEK-293 T cells allowing expression of the spike protein of the highly pathogenic Middle East respiratory syndrome coronavirus in the presence or absence of a cellular trypsin-like transmembrane serine protease, matriptase. Such analysis enables the characterization of cleavage patterns produced by a host protease on a coronavirus spike glycoprotein.
has issue date	2019-09-14(xsd:dateTime)
bibo:doi	10.1007/978-1-0716-0211-9_3
bibo:pmid	31883085
has license	no-cc
sha1sum (hex)	663f4d946f643742c0f16cda102c5f1e6cd67ffb
schema:url	https://doi.org/10.1007/978-1-0716-0211-9_3
resource representing a document's title	Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
has PubMed Central identifier	PMC7121948
has PubMed identifier	31883085
schema:publication	MERS Coronavirus
resource representing a document's body	covid:663f4d946f643742c0f16cda102c5f1e6cd67ffb#body_text
is schema:about of	named entity 'PROTEIN ' named entity 'mediates' named entity 'proteolytically processed' named entity 'essential' named entity 'protein' named entity 'presence' named entity 'coronavirus' named entity 'Middle East Respiratory Syndrome Coronavirus' named entity 'SPIKE PROTEIN' named entity 'CHAPTER' named entity 'FUNCTION' named entity 'VIRUS ENTRY' named entity 'PROTEOLYTIC' named entity 'VISUALIZATION' named entity 'HOST' named entity 'PROCESSED' named entity 'EXPRESSION' named entity 'STRUCTURE' named entity 'ESSENTIAL' named entity 'SPIKE PROTEIN' named entity 'PATTERNS' named entity 'ALLOWING' named entity 'ENZYMES' named entity 'BIOCHEMICAL' named entity 'EVENTS' named entity 'SPIKE' named entity 'ASSESSMENT' named entity 'PRESENT' named entity 'ALLOWS' named entity 'HERE' named entity 'PROTEASE' named entity 'MATRIPTASE' named entity 'PROTEOLYTIC PROCESSING' named entity 'UNDERSTANDING' named entity 'TRANSIENT TRANSFECTION' named entity 'CRITICAL' named entity 'VIRAL COMPONENT' named entity 'ABSENCE OF' named entity 'TRANSMEMBRANE' named entity 'WESTERN BLOT ANALYSIS' named entity 'PATHOGENIC' named entity 'VIRAL LIFE CYCLE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'HEK-293' named entity 'RELATIONSHIPS' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'METHOD' named entity 'CLEAVAGE' named entity 'ACTIVATED' named entity 'CHARACTERIZATION' named entity 'CELLULAR' named entity 'PROTEINS' named entity 'ENDOGENOUS' named entity 'WESTERN BLOT' named entity 'SERINE PROTEASE' named entity 'HOST CELL' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'PROTEIN ' named entity 'CORONAVIRUS' named entity 'MEDIATES' named entity 'TRYPSIN' named entity 'ENABLES' named entity 'HIGHLY' named entity 'T CELLS' named entity 'PROTEASES' named entity 'ANALYSIS' named entity 'EXOGENOUS' named entity 'PRESENCE' named entity 'BASED' named entity 'LIKE' named entity 'BIOCHEMICAL CHARACTERIZATION' named entity 'TO INVESTIGATE' named entity 'PROTEOLYTIC PROCESSING' named entity 'ROLES' named entity 'CATHEPSINS' covid:arg/663f4d946f643742c0f16cda102c5f1e6cd67ffb named entity 'events' named entity 'cleavage' named entity 'western blot' named entity 'virus'

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