About: Liquid chromatography–tandem mass spectrometry analysis of anisodamine and its phase I and II metabolites in rat urine

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About: Liquid chromatography–tandem mass spectrometry analysis of anisodamine and its phase I and II metabolites in rat urine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Liquid chromatography–tandem mass spectrometry analysis of anisodamine and its phase I and II metabolites in rat urine
Creator	Chen, Yong Wang, Hong Chen, Huaixia Zhang, Huashan
Source	PMC
abstract	A sensitive and specific method for the analysis of anisodamine and its metabolites in rat urine by liquid chromatography–electrospray ionization tandem mass spectrometry (LC–MS/MS) was developed. Various extraction techniques (free fraction, acid hydrolyses and enzyme hydrolyses) and their comparison were carried out for investigation of the metabolism of anisodamine. After extraction procedure the pretreated samples were injected on a reversed-phase C18 column with mobile phase (0.2 ml/min) of methanol/0.01% triethylamine solution (adjusted to pH 3.5 with formic acid) (60:40, v/v) and detected by MS/MS. Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses (ΔM), retention-times and full scan MS(n) spectra with those of the parent drug. At least 11 metabolites (N-demethyl-6β-hydroxytropine, 6β-hydroxytropine, tropic acid, N-demethylanisodamine, hydroxyanisodamine, anisodamine N-oxide, hydroxyanisodamine N-oxide, glucuronide conjugated N-demethylanisodamine, sulfate conjugated and glucuronide conjugated anisodamine, sulfate conjugated hydroxyanisodamine) and the parent drug were found in rat urine after the administration of a single oral dose 25 mg/kg of anisodamine. Hydroxyanisodamine, anisodamine N-oxide and the parent drug were detected in rat urine for up 95 h after ingestion of anisodamine.
has issue date	2005-09-25(xsd:dateTime)
bibo:doi	10.1016/j.jchromb.2005.07.036
bibo:pmid	16095983
has license	no-cc
sha1sum (hex)	6692da4f2253bb76154fbb8d809360fd687d935f
schema:url	https://doi.org/10.1016/j.jchromb.2005.07.036
resource representing a document's title	Liquid chromatography–tandem mass spectrometry analysis of anisodamine and its phase I and II metabolites in rat urine
has PubMed Central identifier	PMC7105195
has PubMed identifier	16095983
schema:publication	J Chromatogr B Analyt Technol Biomed Life Sci
resource representing a document's body	covid:6692da4f2253bb76154fbb8d809360fd687d935f#body_text
is schema:about of	named entity 'IDENTIFICATION' named entity 'THEIR' named entity 'ANALYSIS' named entity 'INGESTION' named entity 'RETENTION' named entity 'EXTRACTION TECHNIQUES' named entity 'ELECTROSPRAY IONIZATION' named entity 'SENSITIVE' named entity 'RAT' named entity 'VARIOUS' named entity 'N-OXIDE' named entity 'ITS' named entity 'CARRIED' named entity 'ORAL' named entity 'STRUCTURAL' named entity 'SULFATE' named entity 'PERFORMED BY' named entity 'URINE' named entity 'PHASE I' named entity 'METHANOL' named entity 'FOUND' named entity 'SINGLE' named entity 'COMPARISON' named entity 'TROPIC ACID' named entity 'SPECTRA' named entity 'GLUCURONIDE' named entity 'ACID' named entity 'TRIETHYLAMINE' named entity 'MG%' named entity 'ADMINISTRATION' named entity 'MOLECULAR' named entity 'URINE' named entity '90%' named entity 'CHANGES' named entity 'CONJUGATED' named entity 'DRUG' named entity 'RAT' named entity 'ITS' named entity 'MASS SPECTROMETRY ANALYSIS' named entity 'METABOLITES' named entity 'ANISODAMINE' named entity 'LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY' named entity 'METABOLITES' named entity 'COMPARING' named entity 'DOSE' named entity 'FULL SCAN' named entity 'PH 3.5' named entity 'LIQUID CHROMATOGRAPHY' named entity 'DETECTED' named entity 'PARENT' named entity 'TANDEM MASS SPECTROMETRY' named entity 'REVERSED' named entity 'EXTRACTION PROCEDURE' named entity 'PHASE' named entity 'SOLUTION' named entity 'TIMES' named entity 'MOBILE PHASE' named entity 'FORMIC ACID' named entity 'ENZYME' named entity 'SAMPLES' named entity 'SPECIFIC' named entity 'METHOD' named entity 'INVESTIGATION' named entity 'ANISODAMINE' named entity '2860' named entity 'C18 COLUMN' named entity 'FRACTION' named entity 'anisodamine' named entity 'conjugated' named entity 'urine' named entity 'formic acid' named entity 'mobile phase' named entity 'anisodamine' named entity 'tandem mass spectrometry' named entity 'triethylamine' named entity 'molecular masses' named entity 'pH 3' named entity 'metabolites'

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