About: Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq

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About: Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq
Creator	Liu, Hongwei Eckstrand, Christina Watanabe, Rie Pedersen, Niels
Source	Medline; PMC
abstract	Laboratory cats were infected with a serotype I cat-passaged field strain of FIP virus (FIPV) and peritoneal cells harvested 2–3 weeks later at onset of lymphopenia, fever and serositis. Comparison peritoneal cells were collected from four healthy laboratory cats by peritoneal lavage and macrophages predominated in both populations. Differential mRNA expression analysis identified 5621 genes as deregulated in peritoneal cells from FIPV infected versus normal cats; 956 genes showed > 2.0 Log(2) Fold Change (Log(2)FC) and 1589 genes showed < −2.0 Log(2)FC. Eighteen significantly upregulated pathways were identified by InnateDB enrichment analysis. These pathways involved apoptosis, cytokine–cytokine receptor interaction, pathogen recognition, Jak-STAT signaling, NK cell mediated cytotoxicity, several chronic infectious diseases, graft versus host disease, allograft rejection and certain autoimmune disorders. Infected peritoneal macrophages were activated M1 type based on pattern of RNA expression. Apoptosis was found to involve large virus-laden peritoneal macrophages more than less mature macrophages, suggesting that macrophage death played a role in virus dissemination. Gene transcripts for MHC I but not II receptors were upregulated, while mRNA for receptors commonly associated with virus attachment and identified in other coronaviruses were either not detected (APN, L-SIGN), not deregulated (DDP-4) or down-regulated (DC-SIGN). However, the mRNA for FcγRIIIA (CD16A/ADCC receptor) was significantly upregulated, supporting entry of virus as an immune complex. Analysis of KEGG associated gene transcripts indicated that Th1 polarization overshadowed Th2 polarization, but the addition of relevant B cell associated genes previously linked to FIP macrophages tended to alter this perception.
has issue date	2018-08-07(xsd:dateTime)
bibo:doi	10.1186/s13567-018-0578-y
bibo:pmid	30086792
has license	cc-by
sha1sum (hex)	66a6a5cb7b41fb6edd030629be7c455c079f47bf
schema:url	https://doi.org/10.1186/s13567-018-0578-y
resource representing a document's title	Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq
has PubMed Central identifier	PMC6081860
has PubMed identifier	30086792
schema:publication	Vet Res
resource representing a document's body	covid:66a6a5cb7b41fb6edd030629be7c455c079f47bf#body_text
is schema:about of	named entity 'Analysis' named entity 'virus' named entity 'deregulated' named entity 'Infected' named entity 'Log' named entity 'transcripts' named entity 'peritoneal' named entity 'ADCC' named entity 'down-regulated' named entity 'lymphopenia' named entity 'genes' named entity 'B cell' named entity 'DC-SIGN' named entity 'peritoneal' named entity 'FIP' named entity 'CELLS' named entity 'DEATH' named entity 'THESE' named entity 'COLLECTED' named entity 'VIRUS' named entity 'ADDITION' named entity 'BUT' named entity 'IMMUNE COMPLEX' named entity 'EXPRESSION ANALYSIS' named entity 'RELEVANT' named entity 'ASSOCIATED' named entity 'STRAIN' named entity 'FOLD CHANGE' named entity 'RECEPTORS' named entity 'SUPPORTING' named entity 'B CELL' named entity 'APOPTOSIS' named entity 'PERITONEAL LAVAGE' named entity 'LARGE' named entity 'NORMAL' named entity 'MACROPHAGES' named entity 'ALTER' named entity 'ENRICHMENT ANALYSIS' named entity 'NOT DETECTED' named entity 'NK CELL MEDIATED CYTOTOXICITY' named entity 'JAK' named entity 'HEALTHY' named entity 'GRAFT VERSUS HOST DISEASE' named entity 'PERITONEAL MACROPHAGES' named entity 'PERITONEAL' named entity 'TH2' named entity 'CERTAIN' named entity 'INFECTED' named entity 'FIP' named entity 'LYMPHOPENIA' named entity 'ENTRY' named entity 'GENE TRANSCRIPTS' named entity 'BASED' named entity 'CHRONIC' named entity 'PREDOMINATED' named entity 'ONSET OF' named entity 'PATHWAYS' named entity 'EITHER' named entity 'INDICATED' named entity '3 WEEKS' named entity 'CYTOKINE-CYTOKINE RECEPTOR INTERACTION' named entity 'FEVER' named entity 'FIPV' named entity 'LABORATORY' named entity 'FOUND' named entity 'IDENTIFIED BY' named entity 'PERCEPTION' named entity 'INVOLVED' named entity 'MACROPHAGE' named entity 'INDUCED' named entity 'RECEPTOR' named entity 'RNA EXPRESSION' named entity 'KEGG' named entity 'DEREGULATED' named entity 'CELL ASSOCIATED' covid:arg/66a6a5cb7b41fb6edd030629be7c455c079f47bf named entity 'CHARACTERIZATION' named entity 'PLAYED'

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