About: New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176

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About: New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176 Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176
Creator	Batta, Gyula Bodor, Andrea Bratschun-Khan, Doris Czajlik, András Fizil, Ádám Galgóczy, László Gáspári, Zoltán Hajdu, Dorottya Huber, Anna Kele, Zoltán Marx, Florentine Philippot, Stéphanie Sonderegger, Christoph Varbanov, Mihayl
Source	Medline; PMC
abstract	Small, cysteine-rich and cationic proteins with antimicrobial activity are produced by diverse organisms of all kingdoms and represent promising molecules for drug development. The ancestor of all industrial penicillin producing strains, the ascomycete Penicillium chryosgenum Q176, secretes the extensively studied antifungal protein PAF. However, the genome of this strain harbours at least two more genes that code for other small, cysteine-rich and cationic proteins with potential antifungal activity. In this study, we characterized the pafB gene product that shows high similarity to PgAFP from P. chrysogenum R42C. Although abundant and timely regulated pafB gene transcripts were detected, we could not identify PAFB in the culture broth of P. chrysogenum Q176. Therefore, we applied a P. chrysogenum-based expression system to produce sufficient amounts of recombinant PAFB to address unanswered questions concerning the structure and antimicrobial function. Nuclear magnetic resonance (NMR)-based analyses revealed a compact β-folded structure, comprising five β-strands connected by four solvent exposed and flexible loops and an “abcabc” disulphide bond pattern. We identified PAFB as an inhibitor of growth of human pathogenic moulds and yeasts. Furthermore, we document for the first time an anti-viral activity for two members of the small, cysteine-rich and cationic protein group from ascomycetes.
has issue date	2018-01-29(xsd:dateTime)
bibo:doi	10.1038/s41598-018-20002-2
bibo:pmid	29379111
has license	cc-by
sha1sum (hex)	68405384d530f6beb4fd8cce0841878a72640d28
schema:url	https://doi.org/10.1038/s41598-018-20002-2
resource representing a document's title	New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176
has PubMed Central identifier	PMC5788923
has PubMed identifier	29379111
schema:publication	Sci Rep
resource representing a document's body	covid:68405384d530f6beb4fd8cce0841878a72640d28#body_text
is schema:about of	named entity 'POTENTIAL' named entity 'STRUCTURAL' named entity 'AMOUNTS' named entity 'RECOMBINANT' named entity 'GROUP' named entity 'APPLIED' named entity 'EXPRESSION SYSTEM' named entity 'ADDRESS' named entity 'STRAIN' named entity 'PATHOGENIC' named entity 'DETECTED' named entity 'MEMBERS' named entity 'POTENTIAL' named entity 'FOLDED STRUCTURE' named entity 'YEASTS' named entity 'HUMAN' named entity 'BROTH' named entity 'CODE' named entity 'TIME' named entity 'PENICILLIN' named entity 'STUDY' named entity 'SOLVENT' named entity 'TIMELY' named entity 'PROTEINS' named entity 'CULTURE' named entity 'HIGH' named entity 'SUFFICIENT' named entity 'CYSTEINE' named entity 'GENE PRODUCT' named entity 'GENOME' named entity 'ASCOMYCETE' named entity 'SMALL' named entity 'PRODUCING' named entity 'ASCOMYCETES' named entity 'PENICILLIUM' named entity 'ACTIVITY' named entity 'ANTIMICROBIAL' named entity 'TO PRODUCE' named entity 'BASED' named entity 'EXPOSED' named entity 'VIRAL ACTIVITY' named entity 'PROTEIN ' named entity 'DISULPHIDE BOND' named entity 'ANTIMICROBIAL' named entity 'RICH' named entity 'ANCESTOR' named entity 'GENE TRANSCRIPTS' named entity 'ORGANISMS' named entity 'INDUSTRIAL' named entity 'PATTERN' named entity 'REGULATED' named entity 'IDENTIFY' named entity 'PAFB' named entity 'DIVERSE' named entity 'NEW' named entity 'PENICILLIUM CHRYSOGENUM' named entity 'PROTEIN ' named entity 'ANTIFUNGAL' named entity 'ANTIMICROBIAL ACTIVITY' named entity 'PAFB' named entity 'INHIBITOR' named entity 'STRAINS' named entity 'MOLECULES' named entity 'REVEALED' named entity 'ABUNDANT' named entity 'PAF' named entity 'DOCUMENT ' named entity 'CYSTEINE'

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