About: Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis

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About: Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis
Creator	Li, Zhongwei Chen, Jyunhao Grubbs, Brendan Hallows, Kenneth Huang, Biao Kim, Albert Li, Yidan Mcmahon, Andrew Mcmahon, Jill Parvez, Riana Pastor-Soler, Nuria Patel, Tadrushi Rutledge, Elisabeth Thornton, Matthew Vonk, Ariel Yu, Jingying Zeng, Zipeng
Source	BioRxiv
abstract	Kidney organoids model development and diseases of the nephron but not the contiguous epithelial network of the kidney’s collecting duct (CD) system. Here, we report the generation of an expandable, 3D branching ureteric bud (UB) organoid culture model that can be derived from primary UB progenitors from mouse and human fetal kidneys, or generated de novo from pluripotent human stem cells. UB organoids differentiate into CD organoids in vitro, with differentiated cell types adopting spatial assemblies reflective of the adult kidney collecting system. Aggregating 3D-cultured nephron progenitor cells with UB organoids in vitro results in a reiterative process of branching morphogenesis and nephron induction, similar to kidney development. Combining efficient gene editing with the UB organoid model will facilitate an enhanced understanding of development, regeneration and diseases of the mammalian collecting system. One sentence summary Collecting duct organoids derived from primary mouse and human ureteric bud progenitor cells and human pluripotent stem cells provide an in vitro platform for genetic dissection of development, regeneration and diseases of the mammalian collecting system.
has issue date	2020-04-28(xsd:dateTime)
bibo:doi	10.1101/2020.04.27.049031
has license	biorxiv
sha1sum (hex)	68f39eb7c6482c0224f42c6ab3c39ce11f7c5cc8
schema:url	https://doi.org/10.1101/2020.04.27.049031
resource representing a document's title	Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis
schema:publication	bioRxiv
resource representing a document's body	covid:68f39eb7c6482c0224f42c6ab3c39ce11f7c5cc8#body_text
is schema:about of	named entity 'dissection' named entity 'collecting duct' named entity 'organoids' named entity 'kidney' named entity 'GENCODE' named entity 'aldosterone' named entity 'over-expression' named entity 'UPCs' named entity 'signaling pathways' named entity 'CRISPR' named entity 'organoids' named entity 'genotype' named entity 'cell types' named entity 'therapeutic approaches' named entity 'PAX2' named entity 'transgenic' named entity 'kidney' named entity 'immunostaining' named entity 'kidney development' named entity 'organoid' named entity 'human pluripotent stem cells' named entity 'kidney development' named entity 'mice' named entity 'infection' named entity 'Nephrons' named entity 'organoids' named entity 'luminal' named entity 'cell line' named entity 'organoids' named entity 'self-organize' named entity 'organoid' named entity 'RNA-seq' named entity 'single cells' named entity 'basal' named entity 'branching morphogenesis' named entity 'mCherry' named entity 'Cas9' named entity 'UPCs' named entity 'self-organizing' named entity 'organoids' named entity 'centrifuged' named entity 'PAX2' named entity 'centrifuged' named entity 'qRT-PCR' named entity 'titer' named entity 'organoids' named entity 'PAX2' named entity 'hormonal' named entity 'complement' named entity 'gene editing' named entity 'single cells' named entity 'ICs' named entity 'passaging' named entity 'qRT-PCR' named entity 'Immunostaining' named entity '37°C' named entity 'organoid' named entity 'mCherry' named entity 'single cells' named entity 'progenitor cells' named entity 'Aligned reads' named entity 'distal' named entity 'single cells' named entity 'antibodies' named entity 'branching morphogenesis' named entity 'Human pluripotent stem cells' named entity 'Andrew McMahon' named entity 'GFP'

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