About: Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone

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About: Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone
Creator	Corti, Davide Lanzavecchia, Antonio Minola, Andrea Sadowska, Agnieszka Tkaczyk, Christine Miller, Lloyd Pickett, Julie Sellman, Bret Thompson, John Lj Thorek, Daniel
Source	PMC
abstract	Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody (mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid (LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immunoPET imaging in an in vivo mouse model of prosthetic joint infection (PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography (PET) with [(18)F]FDG and [(18)F]NaF as well as X-ray computed tomography (CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA ([(89)Zr]SAC55). The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater (P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [(89)Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI.
has issue date	2018-04-25(xsd:dateTime)
bibo:doi	10.1038/s41413-018-0014-y
bibo:pmid	29707402
has license	cc-by
sha1sum (hex)	6ac37ecc83bd4e2f6a28476954dd962e10b1b69c
schema:url	https://doi.org/10.1038/s41413-018-0014-y
resource representing a document's title	Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone
has PubMed Central identifier	PMC5916877
has PubMed identifier	29707402
schema:publication	Bone Res
resource representing a document's body	covid:6ac37ecc83bd4e2f6a28476954dd962e10b1b69c#body_text
is schema:about of	named entity 'MODEL' named entity 'TRANSLATE' named entity 'LIPOTEICHOIC ACID' named entity 'ANTIBODY' named entity 'NEEDED' named entity 'CASES' named entity 'X-RAY COMPUTED TOMOGRAPHY' named entity 'ISOTYPE' named entity 'SURFACE' named entity 'BACTERIAL' named entity 'ASSESSED' named entity 'STERILE' named entity 'MORPHOLOGICAL' named entity 'ANTIBODIES' named entity 'USING' named entity 'ASEPTIC LOOSENING' named entity 'S. AUREUS' named entity 'FOLLOWING' named entity 'SPECIFICITY' named entity 'SPECIFIC ANTIBODY' named entity 'ITS' named entity 'PROSTHESIS' named entity 'TAKEN' named entity 'TRADITIONAL' named entity 'FUTURE' named entity 'INCLUDING' named entity 'LABELED' named entity 'MOLECULE' named entity 'PJI' named entity 'DIAGNOSTIC IMAGING' named entity 'POSITIVE' named entity 'BINDING' named entity 'MAB' named entity 'INFLAMMATION' named entity 'INFECTION' named entity 'OUR' named entity 'FINDINGS' named entity 'INFECTED' named entity 'PROSTHETIC JOINT INFECTION' named entity 'DETECTING' named entity 'INJECTION' named entity 'STUDIES' named entity 'antibody' named entity 'probe' named entity 'prosthesis' named entity 'infection' named entity 'positron' named entity 'sites' named entity 'surface' named entity 'diagnostic imaging' named entity 'simulate' named entity 'bone' covid:arg/6ac37ecc83bd4e2f6a28476954dd962e10b1b69c named entity 'OCTET' named entity 'TREATMENT' named entity 'GRAM-' named entity 'BACTERIA.' named entity 'RESULTS' named entity 'SURGICAL SITE' named entity 'SIGNIFICANT' named entity 'ELISA' named entity 'ACTUAL' named entity 'IN VIVO' named entity 'EITHER' named entity 'MOUSE MODEL' named entity 'EFFECTIVENESS' named entity 'HUMAN' named entity 'IMAGING TOOL' named entity 'CONTROL' named entity 'SPECIFIC' named entity 'FLOW CYTOMETRY' named entity 'ZIRCONIUM-89'

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