About: A duplex real-time RT-PCR assay for the detection of St. Louis encephalitis and Eastern equine encephalitis viruses

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About: A duplex real-time RT-PCR assay for the detection of St. Louis encephalitis and Eastern equine encephalitis viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A duplex real-time RT-PCR assay for the detection of St. Louis encephalitis and Eastern equine encephalitis viruses
Creator	Kramer, Laura Hull, Rene Nattanmai, Seela Bernard, Kristen Tavakoli, Norma
Source	Elsevier; Medline; PMC
abstract	A duplex TaqMan real-time RT-PCR assay was developed for the detection of St. Louis encephalitis virus (SLEV) and Eastern equine encephalitis virus (EEEV), for use in human and vector surveillance. The respective targets selected for the assay were the conserved NS5 and E1 genes of the two viruses. Due to the insufficient number of NS5 sequences from SLEV strains in the GenBank database, we determined the sequence of an approximately 1-kb region for each of 25 strains of SLEV in order to select primers and probes in a conserved region. Our assay has a sensitivity of 5 gene copies/reaction for EEEV and 10 gene copies/reaction for SLEV, and it’s performance is linear over at least 6 log(10) gene copies. The assay is specific and detected all strains of SLEV (69) and EEEV (12) that were tested. An internal control ensures detection of efficient nucleic acid extraction and possible PCR inhibition.
has issue date	2008-11-01(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2008.07.004
bibo:pmid	18715737
has license	green-oa
sha1sum (hex)	6b4b32c9c9f84ee5d0a74196f5d9378a955adac1
schema:url	https://doi.org/10.1016/j.diagmicrobio.2008.07.004
resource representing a document's title	A duplex real-time RT-PCR assay for the detection of St. Louis encephalitis and Eastern equine encephalitis viruses
has PubMed Central identifier	PMC2615585
has PubMed identifier	18715737
schema:publication	Diagnostic Microbiology and Infectious Disease
resource representing a document's body	covid:6b4b32c9c9f84ee5d0a74196f5d9378a955adac1#body_text
is schema:about of	named entity 'gene' named entity 'copies' named entity 'PCR' named entity 'conserved' named entity 'chain reaction' named entity 'equine encephalitis' named entity 'ENSURES' named entity 'REAL-TIME' named entity 'CONSERVED' named entity 'ST. LOUIS ENCEPHALITIS VIRUS' named entity 'SELECTED' named entity 'SELECT' named entity 'DETECTED' named entity 'EASTERN EQUINE ENCEPHALITIS VIRUS' named entity 'INTERNAL' named entity 'CONSERVED REGION' named entity 'NS5' named entity 'LINEAR' named entity 'DETECTION' named entity 'PCR' named entity 'CONTROL' named entity 'APPROXIMATELY' named entity 'SPECIFIC' named entity 'DUPLEX' named entity 'SENSITIVITY' named entity 'VIRUSES' named entity 'ST. LOUIS ENCEPHALITIS' named entity 'OUR' named entity 'HUMAN' named entity 'ITS' named entity 'TARGETS' named entity 'PUBLISHED' named entity 'POLYMERASE CHAIN REACTION ASSAY' named entity 'EASTERN EQUINE ENCEPHALITIS ' named entity 'ENCEPHALITIS VIRUSES' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'TESTED' named entity 'EFFICIENT' named entity 'PERFORMANCE' named entity 'GENES' named entity 'GENE' named entity 'DUPLEX' named entity 'POSSIBLE' named entity 'REGION' named entity 'STRAINS' named entity 'PRIMERS' named entity 'USE' named entity 'DETECTION' named entity 'REAL-TIME' named entity 'INHIBITION' named entity 'TAQMAN' named entity 'GENBANK' named entity 'SEQUENCES' named entity 'LOG' named entity 'NUMBER OF' named entity 'SEQUENCE' named entity 'ASSAY' named entity 'DATABASE' named entity 'COPIES' named entity 'PROBES' named entity 'INC' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'INSUFFICIENT' named entity 'DETERMINED' named entity 'NUCLEIC ACID EXTRACTION' covid:arg/6b4b32c9c9f84ee5d0a74196f5d9378a955adac1 named entity 'internal control' named entity 'RNA transcripts' named entity 'EEEV' named entity 'Massachusetts' named entity 'RSV' named entity 'EEEV' named entity 'cDNA' named entity 'vertebrates' named entity 'EEEV' named entity '0.01' named entity 'RNA'

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