About: Actinobacillus pleuropneumoniae Possesses an Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Actinobacillus pleuropneumoniae Possesses an Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus
Creator	Labrie, J Lévesque, C Nava, Burciaga Provost, C Reyes, Hernandez
source	PMC
abstract	Pigs are often colonized by more than one bacterial and/or viral species during respiratory tract infections. This phenomenon is known as the porcine respiratory disease complex (PRDC). Actinobacillus pleuropneumoniae (App) and porcine reproductive and respiratory syndrome virus (PRRSV) are pathogens that are frequently involved in PRDC. The main objective of this project was to study the in vitro interactions between these two pathogens and the host cells in the context of mixed infections. To fulfill this objective, PRRSV permissive cell lines such as MARC-145, SJPL, and porcine alveolar macrophages (PAM) were used. A pre-infection with PRRSV was performed at 0.5 multiplicity of infection (MOI) followed by an infection with App at 10 MOI. Bacterial adherence and cell death were compared. Results showed that PRRSV pre-infection did not affect bacterial adherence to the cells. PRRSV and App co-infection produced an additive cytotoxicity effect. Interestingly, a pre-infection of SJPL and PAM cells with App blocked completely PRRSV infection. Incubation of SJPL and PAM cells with an App cell-free culture supernatant is also sufficient to significantly block PRRSV infection. This antiviral activity is not due to LPS but rather by small molecular weight, heat-resistant App metabolites (<1 kDa). The antiviral activity was also observed in SJPL cells infected with swine influenza virus but to a much lower extent compared to PRRSV. More importantly, the PRRSV antiviral activity of App was also seen with PAM, the cells targeted by the virus in vivo during infection in pigs. The antiviral activity might be due, at least in part, to the production of interferon γ. The use of in vitro experimental models to study viral and bacterial co-infections will lead to a better understanding of the interactions between pathogens and their host cells, and could allow the development of novel prophylactic and therapeutic tools.
has issue date	2014-05-30(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0098434
bibo:pmid	24878741
has license	cc-by
sha1sum (hex)	6bba4b00882498efa69f66a1a92b1c240acfd2a9
schema:url	https://doi.org/10.1371/journal.pone.0098434
resource representing a document's title	Actinobacillus pleuropneumoniae Possesses an Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus
has PubMed Central identifier	PMC4039538
has PubMed identifier	24878741
schema:publication	PLoS One
resource representing a document's body	covid:6bba4b00882498efa69f66a1a92b1c240acfd2a9#body_text
is schema:about of	named entity 'reproductive' named entity 'bacterial' named entity 'activity' named entity 'compared' named entity 'Pigs' named entity 'cells' named entity 'This' named entity 'Activity' named entity 'Porcine' named entity 'RESISTANT' named entity 'HEAT' named entity 'STUDY' named entity 'CONTEXT' named entity 'CELLS' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'MUCH' named entity 'LEAD' named entity 'PHENOMENON' named entity 'BLOCK' named entity '0.5' named entity 'COMPARED' named entity 'DID' named entity 'IN VIVO' named entity 'BLOCKED' named entity 'MARC' named entity 'MIGHT BE' named entity 'UNDERSTANDING' named entity 'ALVEOLAR MACROPHAGES' named entity 'INTERACTIONS' named entity 'COMPLEX' named entity 'FULFILL' named entity 'RATHER' named entity 'PATHOGENS' named entity 'COMPLETELY' named entity 'ALLOW' named entity 'SEEN' named entity 'VIRUS' named entity 'POSSESSES' named entity 'ACTINOBACILLUS PLEUROPNEUMONIAE' named entity 'CELL LINES' named entity 'SWINE INFLUENZA VIRUS' named entity 'INFECTED' named entity 'PRDC' named entity 'IN VITRO' named entity 'RESPIRATORY DISEASE' named entity 'INCUBATION' named entity 'CELL' named entity 'MULTIPLICITY' named entity 'INTERFERON ' named entity 'MAIN' named entity 'KNOWN' named entity 'THEIR' named entity 'RESPIRATORY TRACT INFECTIONS' named entity 'CO-INFECTION' named entity 'EXTENT' named entity 'EXPERIMENTAL MODELS' named entity 'MOLECULAR WEIGHT' named entity 'CELL DEATH' named entity 'PRE-INFECTION' named entity 'MIXED' named entity 'EFFECT' named entity 'INVOLVED' named entity 'DEVELOPMENT' named entity 'USED' named entity 'PIGS' named entity 'TARGETED' named entity 'AT 10' named entity 'INFECTION' named entity 'PROPHYLACTIC' named entity 'BUT' named entity 'THESE' named entity 'SUFFICIENT' named entity 'BETTER' named entity 'THERAPEUTIC' named entity 'PAM' named entity 'VIRAL AND' named entity 'ADDITIVE'

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