About: Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR

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About: Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR
Creator	Zheng, Li-Shu Li, Jin-Song Li, Dan-Di Ma, Fen-Lian Wei, Tian-Li
Source	Medline; PMC
abstract	BACKGROUND: Human Malawi polyomavirus (MWPyV) was discovered in 2012, but its prevalence and clinical characteristics are largely unknown. METHODS: We used real-time TaqMan-based PCR to detect MWPyV in the feces (n = 174) of children with diarrhea, nasopharyngeal aspirates (n = 887) from children with respiratory infections, and sera (n = 200) from healthy adults, and analyzed its clinical characteristics statistically. All the MWPyV-positive specimens were also screened for other common respiratory viruses. RESULTS: Sixteen specimens were positive for MWPyV, including 13 (1.47%) respiratory samples and three (1.7%) fecal samples. The samples were all co-infected with other respiratory viruses, most commonly with influenza viruses (69.2%) and human coronaviruses (30.7%). The MWPyV-positive children were diagnosed with bronchopneumonia or viral diarrhea. They ranged in age from 12 days to 9 years, and the most frequent symptoms were cough and fever. CONCLUSIONS: Real-time PCR is an effective tool for the detection of MWPyV in different types of samples. MWPyV infection mainly occurs in young children, and fecal–oral transmission is a possible route of its transmission. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-017-0817-2) contains supplementary material, which is available to authorized users.
has issue date	2017-08-14(xsd:dateTime)
bibo:doi	10.1186/s12985-017-0817-2
bibo:pmid	28806976
has license	cc-by
sha1sum (hex)	6cc5fe5c208412e13970cc93efbd1e68d1fe6459
schema:url	https://doi.org/10.1186/s12985-017-0817-2
resource representing a document's title	Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR
has PubMed Central identifier	PMC5557062
has PubMed identifier	28806976
schema:publication	Virol J
resource representing a document's body	covid:6cc5fe5c208412e13970cc93efbd1e68d1fe6459#body_text
is schema:about of	named entity 'TIME' named entity 'POLYOMAVIRUS' named entity 'DETECTION' named entity 'SERA' named entity 'NASOPHARYNGEAL' named entity 'QUANTITATIVE' named entity 'USING' named entity 'BASED' named entity 'REAL-TIME' named entity 'CHILDREN' named entity 'FECES' named entity 'RESPIRATORY VIRUSES' named entity '200' named entity 'HEALTHY' named entity 'METHODS' named entity 'SERA' named entity 'PREVALENCE' named entity 'RESPIRATORY INFECTIONS' named entity 'BACKGROUND' named entity 'TO DETECT' named entity 'SPECIMENS' named entity 'UNKNOWN' named entity 'ASPIRATES' named entity 'BUT' named entity 'NASOPHARYNGEAL' named entity 'BASED' named entity 'ITS' named entity 'POSITIVE' named entity 'MALAWI' named entity 'STATISTICALLY' named entity 'HUMAN' named entity 'TAQMAN' named entity 'FECES' named entity 'REAL' named entity 'CHINA' named entity 'BEIJING' named entity 'TAQMAN' named entity 'HUMAN' named entity '174' named entity 'ADULTS' named entity 'ANALYZED' named entity 'CLINICAL CHARACTERISTICS' named entity 'ASPIRATES' named entity 'USED' named entity 'LARGELY' named entity 'MALAWI' named entity 'PCR' named entity '887' named entity 'PCR' named entity 'POLYOMAVIRUS' named entity 'COMMON' named entity 'DIARRHEA' named entity 'PCR' named entity 'detect' named entity 'discovered' named entity 'respiratory infections' named entity 'Malawi' named entity 'TaqMan' named entity 'diarrhea' named entity 'PCR' named entity 'nasopharyngeal' named entity 'nasopharyngeal' named entity '4.2' named entity 'co-infected' named entity 'negative control' named entity 'JCV' named entity 'infection' named entity 'BKPyV' named entity 'RSV' named entity 'Czech Republic' named entity 'MWPyV' named entity 'MWPyV' named entity 'virus' named entity 'RT-PCR' named entity 'ARIs' named entity 'gastrointestinal' named entity 'qPCR'

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