About: Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery

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About: Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
Creator	Montes, Johnny Schulze, Erica Tran, Rosa Chahal, Parminder Kamen, Amine
Source	Elsevier; Medline; PMC
abstract	Abstract Adeno-associated virus (AAV) is being used successfully in gene therapy. Different serotypes of AAV target specific organs and tissues with high efficiency. There exists an increasing demand to manufacture various AAV serotypes in large quantities for pre-clinical and clinical trials. A generic and scalable method has been described in this study to efficiently produce AAV serotypes (AAV1-9) by transfection of a fully characterized cGMP HEK293SF cell line grown in suspension and serum-free medium. First, the production parameters were evaluated using AAV2 as a model serotype. Second, all nine AAV serotypes were produced successfully with yields of 1013 Vg/L cell culture. Subsequently, AAV2 and AAV6 serotypes were produced in 3-L controlled bioreactors where productions yielded up to 1013 Vg/L similar to the yields obtained in shake-flasks. For example, for AAV2 1013 Vg/L cell culture (6.8×1011 IVP/L) were measured between 48 and 64h post transfection (hpt). During this period, the average cell specific AAV2 yields of 6800Vg per cell and 460IVP per cell were obtained with a Vg to IVP ratio of less than 20. Successful operations in bioreactors demonstrated the potential for scale-up and industrialization of this generic process for manufacturing AAV serotypes efficiently.
has issue date	2014-02-28(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.10.038
bibo:pmid	24239634
has license	els-covid
sha1sum (hex)	6cf5095850e83d633002293144c1162712cf2644
schema:url	https://doi.org/10.1016/j.jviromet.2013.10.038
resource representing a document's title	Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
has PubMed Central identifier	PMC7113661
has PubMed identifier	24239634
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:6cf5095850e83d633002293144c1162712cf2644#body_text
is schema:about of	named entity 'demonstrated' named entity 'potential' named entity 'suspension' covid:arg/6cf5095850e83d633002293144c1162712cf2644 named entity 'serotypes' named entity 'period' named entity 'bioreactors' named entity 'target' named entity 'serotypes' named entity 'efficiently' named entity 'L cell' named entity 'AAV' named entity 'capsids' named entity 'proof of concept' named entity 'butyric acid' named entity 'AAV' named entity 'supernatant' named entity 'serum' named entity 'AAV' named entity 'culture' named entity 'specific' named entity 'For' named entity 'IVP' named entity 'industrialization' named entity 'yields' named entity 'AAV2' named entity 'AAV' named entity 'AAV' named entity 'bioreactors' named entity 'clinical trials' named entity 'Adeno-associated virus' named entity 'AAV' named entity 'bioreactors' named entity 'AAV2' named entity 'serotypes' named entity 'target specific' named entity 'serotypes' named entity 'AAV' named entity 'serotypes' named entity 'adeno-associated virus' named entity 'AAV' named entity 'HEK293' named entity 'transfection' named entity 'AAV2' named entity 'AAV2' named entity 'cell culture' named entity 'transgene' named entity 'butyric acid' named entity 'expression levels' named entity 'AAV9' named entity 'AAV8' named entity 'AAV' named entity 'AAV' named entity 'HIV' named entity 'serotypes' named entity 'bioreactor' named entity 'serum' named entity 'transfection' named entity 'transfection' named entity 'cell culture' named entity 'virus' named entity 'Dissolved oxygen' named entity 'denaturation' named entity 'transgene' named entity 'mode of production' named entity 'helper virus' named entity 'supernatant' named entity 'transfecting' named entity 'plasmid' named entity 'transfection' named entity 'AAV' named entity 'serotypes' named entity 'trypsin' named entity 'Germany' named entity 'scAAV' named entity 'amine' named entity 'plasmids'

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