About: Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice

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About: Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice
Creator	Zhou, Rui Huang, Jing Liu, Xiao Huang, Chao Li, Lu Qi, Yanhua Wang, Antian Yang, Xi
Source	Medline; PMC
abstract	BACKGROUND: Porcine β-defensin 2 (PBD-2), produced by host cells, is an antimicrobial cysteine-rich cationic peptide with multi-functions. Previous studies have demonstrated that PBD-2 can kill various bacteria, regulate host immune responses and promote growth of piglets. However, the antiviral role of PBD-2 is rarely investigated. This study aimed to reveal the antiviral ability of PBD-2 against pseudorabies virus (PRV), the causative pathogen of Aujeszky’s disease, in PK-15 cells and in a PBD-2 expressing transgenic (TG) mouse model. METHODS: In this study, the cytotoxicity of PBD-2 on PK-15 cells was measured by CCK-8 assay. PK-15 cells were incubated with PRV pre-treated with different concentrations of PBD-2 and PRV titers in cell culture supernatants were determined by real-time quantitative PCR (RT-qPCR). TG mice and wild-type (WT) mice were intraperitoneally injected with PRV and the survival rate was recorded for 10 days. Meanwhile, tissue lesions in brain, spleen and liver of infected mice were observed and the viral loads of PRV in brain, liver and lung were analyzed by RT-qPCR. RESULTS: PBD-2 at a maximum concentration of 80 μg/mL displayed no significant cytotoxicity on PK-15 cells. A threshold concentration of PBD-2 at 40 μg/mL was required to inhibit PRV proliferation in PK-15 cells. The survival rate in PBD-2 TG mice was 50% higher than that of WT mice. In addition, TG mice showed alleviated tissue lesions in brain, spleen and liver compared with their WT littermates after PRV challenge, while viral loads of PRV in brain, liver and lung of TG mice were significantly lower than that of WT mice. CONCLUSIONS: PBD-2 could inhibit PRV proliferation in PK-15 cells and protect mice from PRV infection, which confirmed the antiviral ability of PBD-2 both in vitro and in vivo. The application of PBD-2 in developing anti-viral drugs or disease-resistant animals can be further investigated.
has issue date	2020-02-03(xsd:dateTime)
bibo:doi	10.1186/s12985-020-1288-4
bibo:pmid	32014007
has license	cc-by
sha1sum (hex)	6f67e5b5e61f9ce94ddc8aaae34799a81c4c213d
schema:url	https://doi.org/10.1186/s12985-020-1288-4
resource representing a document's title	Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice
has PubMed Central identifier	PMC6998849
has PubMed identifier	32014007
schema:publication	Virol J
resource representing a document's body	covid:6f67e5b5e61f9ce94ddc8aaae34799a81c4c213d#body_text
is schema:about of	named entity 'quantitative PCR' named entity 'cytotoxicity' named entity 'cationic' named entity 'survival rate' named entity 'PBD' named entity 'mouse' named entity 'reveal' named entity 'pathogen' named entity 'PBD' named entity 'virus' named entity 'inhibits' named entity 'β-defensin' covid:arg/6f67e5b5e61f9ce94ddc8aaae34799a81c4c213d named entity 'host' named entity 'piglets' named entity 'tissue' named entity 'PRV' named entity 'study' named entity 'studies' named entity 'PBD' named entity 'measured' named entity 'causative' named entity 'PBD' named entity 'cationic' named entity 'lung' named entity 'cytotoxicity' named entity 'wild-type' named entity 'CCK-8' named entity 'PRV' named entity 'PRV' named entity 'bacteria' named entity 'supernatants' named entity 'pseudorabies virus' named entity 'β-defensin' named entity 'transgenic mice' named entity 'vaccination' named entity 'copy numbers' named entity 'gene' named entity 'pig' named entity 'PRV' named entity 'sequence alignment' named entity 'China' named entity 'liver' named entity 'infection' named entity 'HNP-1' named entity 'defensins' named entity 'β-defensin' named entity 'Microglia' named entity 'lung' named entity 'necrosis' named entity 'β-defensin' named entity 'FBS' named entity 'tissue tropism' named entity 'Zürich' named entity 'HNP' named entity 'histopathological' named entity 'survival rate' named entity 'infection' named entity 'liver' named entity 'HSV' named entity 'infection' named entity 'zebrafish' named entity 'transgenic' named entity 'pig' named entity '96-well plate' named entity 'virucidal' named entity 'Bovine' named entity 'lung' named entity 'mice' named entity 'Shanghai' named entity 'vertebrates' named entity 'infection' named entity 'PRV' named entity 'lung'

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