About: A Transient Homotypic Interaction Model for the Influenza A Virus NS1 Protein Effector Domain

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Transient Homotypic Interaction Model for the Influenza A Virus NS1 Protein Effector Domain
Creator	Digard, Paul García-Sastre, Adolfo Ayllon, Juan Hass, Claudia Lewis, Andrew Hale, Benjamin Kerry, Philip Randall, Richard Russell, Rupert Taylor, Margaret
source	Medline; PMC
abstract	Influenza A virus NS1 protein is a multifunctional virulence factor consisting of an RNA binding domain (RBD), a short linker, an effector domain (ED), and a C-terminal ‘tail’. Although poorly understood, NS1 multimerization may autoregulate its actions. While RBD dimerization seems functionally conserved, two possible apo ED dimers have been proposed (helix-helix and strand-strand). Here, we analyze all available RBD, ED, and full-length NS1 structures, including four novel crystal structures obtained using EDs from divergent human and avian viruses, as well as two forms of a monomeric ED mutant. The data reveal the helix-helix interface as the only strictly conserved ED homodimeric contact. Furthermore, a mutant NS1 unable to form the helix-helix dimer is compromised in its ability to bind dsRNA efficiently, implying that ED multimerization influences RBD activity. Our bioinformatical work also suggests that the helix-helix interface is variable and transient, thereby allowing two ED monomers to twist relative to one another and possibly separate. In this regard, we found a mAb that recognizes NS1 via a residue completely buried within the ED helix-helix interface, and which may help highlight potential different conformational populations of NS1 (putatively termed ‘helix-closed’ and ‘helix-open’) in virus-infected cells. ‘Helix-closed’ conformations appear to enhance dsRNA binding, and ‘helix-open’ conformations allow otherwise inaccessible interactions with host factors. Our data support a new model of NS1 regulation in which the RBD remains dimeric throughout infection, while the ED switches between several quaternary states in order to expand its functional space. Such a concept may be applicable to other small multifunctional proteins.
has issue date	2011-03-28(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0017946
bibo:pmid	21464929
has license	cc-by
sha1sum (hex)	6fad5703e838cae052bc9cd18b32ea812b942fb4
schema:url	https://doi.org/10.1371/journal.pone.0017946
resource representing a document's title	A Transient Homotypic Interaction Model for the Influenza A Virus NS1 Protein Effector Domain
has PubMed Central identifier	PMC3065461
has PubMed identifier	21464929
schema:publication	PLoS One
resource representing a document's body	covid:6fad5703e838cae052bc9cd18b32ea812b942fb4#body_text
is schema:about of	named entity 'compromised' named entity 'NS1' named entity 'mAb' named entity 'short' named entity 'NS1' named entity 'NS1' named entity 'interface' named entity 'quaternary' named entity 'actions' named entity 'Domain' named entity 'Protein' named entity 'INTERACTION MODEL' named entity 'WORK' named entity 'APPLICABLE' named entity 'LINKER' named entity 'PROTEIN ' named entity 'ACTIVITY' named entity 'ENHANCE' named entity 'POTENTIAL' named entity 'FOUND' named entity 'RELATIVE TO' named entity 'INCLUDING' named entity 'INFECTION' named entity 'NEW' named entity 'APO' named entity 'RNA BINDING DOMAIN' named entity 'CONTACT' named entity 'APPEAR' named entity 'INTERACTIONS' named entity 'LEWIS A' named entity 'REVEAL' named entity 'DIMERIZATION' named entity 'PROTEINS' named entity 'OPEN' named entity 'DIMER' named entity 'OBTAINED' named entity 'HELIX' named entity 'VARIABLE' named entity 'DIFFERENT' named entity 'DATA' named entity 'BIND' named entity 'VIRULENCE FACTOR' named entity 'FORMS' named entity 'RBD' named entity 'HOST' named entity 'INTERACTION MODEL' named entity 'SEPARATE' named entity 'MODEL OF' named entity 'DSRNA BINDING' named entity 'TWIST' named entity 'AVAILABLE' named entity 'NOVEL' named entity 'HUMAN' named entity 'VIRUS' named entity 'INFLUENZA A VIRUS' named entity 'ALLOW' named entity 'INFLUENZA A VIRUS' named entity 'DOMAIN' named entity 'AVIAN' named entity 'CLOSED' named entity 'STATES' named entity 'CRYSTAL' named entity 'TRANSIENT' named entity 'DSRNA' named entity 'MONOMERS' named entity 'HELP' named entity 'RESIDUE' named entity 'DIMERS' named entity 'PROPOSED' named entity 'EFFECTOR' named entity 'POORLY' named entity 'INFECTED'

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