About: Role of fusion protein cleavage site in the virulence of Newcastle disease virus

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About: Role of fusion protein cleavage site in the virulence of Newcastle disease virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Role of fusion protein cleavage site in the virulence of Newcastle disease virus
Creator	Elankumaran, Subbiah Huang, Zhuhui Panda, Aruna Samal, Siba Rockemann, Daniel
Source	Elsevier; Medline; PMC
abstract	Abstract Newcastle disease virus (NDV) causes a highly contagious and economically important disease in poultry. Viral determinants of NDV virulence are not completely understood. The amino acid sequence at the protease cleavage site of the fusion (F) protein has been postulated as a major determinant of NDV virulence. In this study, we have examined the role of F protein cleavage site sequence in NDV virulence using reverse genetics technology. The sequence G-R-Q-G-R present at the cleavage site of the F protein of avirulent strain LaSota was mutated to R-R-Q-K-R, which is present in the F cleavage site of neurovirulent strain Beaudette C (BC). The resultant mutated LaSota V.F. virus did not require exogenous protease for infectivity in cell culture, indicating that the F protein was cleaved by intracellular proteases. The virulence of the mutant and parental viruses was evaluated in vivo by intracerebral pathogenicity index (ICPI) and intravenous pathogenicity index (IVPI) tests in chickens. Our results showed that the modification of the F protein cleavage site resulted in a dramatic increase in virulence from an ICPI value of 0.00 for LaSota to a value of 1.12 for LaSota V.F. However, the ICPI value of LaSota V.F. was lower than that of BC, which had a value of 1.58. Interestingly, the IVPI tests showed values of 0.00 for both LaSota and LaSota V.F. viruses, compared to the IVPI value of 1.45 of BC. In vitro characteristics of the viruses were also studied. Our results demonstrate that the efficiency of cleavage of the F protein plays an important role if the NDV is delivered directly into the brains of chicks, but there could be other viral factors that probably affect peripheral replication, viremia, or entry into the central nervous system.
has issue date	2004-01-31(xsd:dateTime)
bibo:doi	10.1016/j.micpath.2003.07.003
bibo:pmid	14643634
has license	els-covid
sha1sum (hex)	6fba236804cbfe944da479bb6e82abb0c53b47a0
schema:url	https://doi.org/10.1016/j.micpath.2003.07.003
resource representing a document's title	Role of fusion protein cleavage site in the virulence of Newcastle disease virus
has PubMed Central identifier	PMC7125746
has PubMed identifier	14643634
schema:publication	Microbial Pathogenesis
resource representing a document's body	covid:6fba236804cbfe944da479bb6e82abb0c53b47a0#body_text
is schema:about of	named entity 'neurovirulent' named entity 'strain' named entity 'resultant' named entity 'cell culture' named entity 'reverse genetics' named entity 'cleavage' named entity 'Newcastle disease virus' named entity 'VIRULENCE' named entity 'FUSION PROTEIN' named entity 'IN VIVO' named entity 'HIGHLY' named entity 'TESTS' named entity 'PLAYS' named entity 'VALUE' named entity 'MUTANT' named entity 'TECHNOLOGY' named entity 'REVERSE GENETICS' named entity 'CHARACTERISTICS' named entity 'AVIRULENT' named entity 'PARENTAL' named entity 'CAUSES' named entity 'FUSION' covid:arg/6fba236804cbfe944da479bb6e82abb0c53b47a0 named entity 'INFECTIVITY' named entity 'BUT' named entity 'INTRACEREBRAL' named entity 'DIRECTLY' named entity 'ECONOMICALLY IMPORTANT' named entity 'PROTEIN ' named entity 'INTRAVENOUS' named entity 'OUR' named entity 'IMPORTANT' named entity 'INTRACELLULAR' named entity 'EFFICIENCY' named entity 'A MAJOR' named entity 'CHICKENS' named entity 'PATHOGENICITY' named entity 'COMPLETELY' named entity 'BRAINS' named entity 'COMPARED' named entity 'INCREASE' named entity 'AFFECT' named entity 'CELL CULTURE' named entity 'PROTEASE' named entity 'ROLE' named entity 'PROTEIN CLEAVAGE' named entity 'DISEASE' named entity 'EXOGENOUS' named entity 'DID' named entity 'EVALUATED' named entity '1.45' named entity 'UNDERSTOOD' named entity 'PROTEASE CLEAVAGE' named entity 'CLEAVAGE' named entity 'PERIPHERAL' named entity 'MUTATED' named entity 'SEQUENCE' named entity 'VIRAL' named entity 'USING' named entity 'STUDY' named entity 'VIREMIA' named entity 'VIRULENCE' named entity 'RESULTS' named entity 'SITE' named entity 'DETERMINANT' named entity 'LOWER' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'PROBABLY' named entity 'SITE OF' named entity 'CHICKS' named entity 'COULD BE' named entity 'PROTEIN CLEAVAGE' named entity 'MODIFICATION' named entity 'AMINO ACID SEQUENCE' named entity 'VIRUSES' named entity 'INDICATING' named entity 'HAVE'

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