About: Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
Creator	Liu, Yong Liu, Ying Gao, Ya Li, Wendong Liu, Chunsheng Wen, Hao Zhu, Xiaoqing
Source	PMC
abstract	The rate-limiting enzyme in the mevalonic acid (MVA) pathway which can lead to triterpenoid saponin glycyrrhizic acid (GA) is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). In order to reveal the effect of copy number variation in the HMGR gene on the MVA pathway, the HMGR gene from Glycyrrhiza uralensis Fisch. (GuHMGR) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy numbers of the GuHMGR gene were obtained and the content of ergosterol was analyzed by HPLC. The results showed that all the recombinant P. pastoris strains contained more ergosterol than the negative control and the strains with 8 and 44 copies contained significantly more ergosterol than the other strains. However, as the copy number increased, the content of ergosterol showed an increasing–decreasing–increasing pattern. This study provides a rationale for increasing the content of GA through over-expressing the GuHMGR gene in cultivars of G. uralensis.
has issue date	2014-04-02(xsd:dateTime)
bibo:doi	10.1016/j.apsb.2014.02.007
bibo:pmid	26579379
has license	cc-by-nc-nd
sha1sum (hex)	70b3836f7b7cc9c445be26a0b3de2455711c7a39
schema:url	https://doi.org/10.1016/j.apsb.2014.02.007
resource representing a document's title	Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis
has PubMed Central identifier	PMC4590296
has PubMed identifier	26579379
schema:publication	Acta Pharm Sin B
resource representing a document's body	covid:70b3836f7b7cc9c445be26a0b3de2455711c7a39#body_text
is schema:about of	named entity '3-HYDROXY-3-METHYLGLUTARYL COA REDUCTASE' named entity 'GLYCYRRHIZA URALENSIS' named entity 'STUDY' named entity 'DECREASING' named entity 'LEAD' named entity 'RATIONALE' named entity 'NUMBERS' named entity 'COPY NUMBER' named entity 'STRAINS' named entity 'ANALYZED' named entity 'REVEAL' named entity 'OBTAINED' named entity 'GLYCYRRHIZIC ACID' named entity 'PATHWAY' named entity 'OVER-EXPRESSED' named entity 'ERGOSTEROL' named entity 'ENHANCING' named entity 'PICHIA PASTORIS' named entity 'PRODUCTION' named entity 'ENZYME' named entity 'GENE' named entity 'RESULTS' named entity 'EXPRESSION' named entity 'INCREASED' named entity 'HPLC' named entity 'INCREASING' named entity 'COPY NUMBER VARIATION' named entity 'PICHIA PASTORIS' named entity 'ERGOSTEROL' named entity 'TRITERPENOID SAPONIN' named entity 'NEGATIVE CONTROL' named entity 'DIFFERENT' named entity '3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE' named entity 'CLONED' named entity 'FISCH' named entity 'CONTENT' named entity 'PROVIDES' named entity 'MVA PATHWAY' named entity 'COPY' named entity 'RECOMBINANT' named entity 'ORDER' named entity 'EFFECT' named entity 'RATE-LIMITING' named entity 'MEVALONIC ACID' named entity 'GLYCYRRHIZA URALENSIS' named entity 'PATTERN' named entity 'CONTAINING' covid:arg/70b3836f7b7cc9c445be26a0b3de2455711c7a39 named entity 'gene' named entity 'recombinant' named entity 'glycyrrhizic acid' named entity 'gene' named entity '3-hydroxy-3-methylglutaryl-CoA reductase' named entity 'Glycyrrhiza uralensis' named entity 'copy number variation' named entity 'rate-limiting enzyme' named entity 'HPLC' named entity 'P. pastoris' named entity 'ergosterol' named entity '3-hydroxy-3-methylglutaryl CoA' named entity 'ergosterol' named entity 'ergosterol' named entity 'ergosterol' named entity 'G. uralensis' named entity 'CNV' named entity 'absolute ethanol' named entity 'P. pastoris' named entity 'negative control' named entity 'CoA' named entity 'phenotypic character' named entity 'herb' named entity 'P. pastoris' named entity 'ergosterol' named entity 'gene' named entity 'P. pastoris'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software