About: Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples

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About: Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples
Creator	Kim, Jae-Seok Song, Wonkeun Kim, Hyun Kim, Han-Sung Kim, Jayoung Lee, Sunhwa Lee, Woochang Hong, Young Park, Kyoung, Un Lee, Kyu
Source	Medline; PMC
abstract	BACKGROUND: We evaluated the analytical and clinical performances of the SD BIOLINE Rota/Adeno Rapid kit (SD Rota/Adeno Rapid; Standard Diagnostics, Inc., Korea), an immunochromatographic assay (ICA), for the simultaneous detection of rotaviruses and adenoviruses in human stool samples. METHODS: We tested 400 clinical stool samples from patients with acute gastroenteritis and compared the ICA results with the results obtained by using ELISA, enzyme-linked fluorescent assays (ELFA), PCR, and multiplex reverse transcription-PCR (mRT-PCR). To assess the analytical performance of the SD BIOLINE Rota/Adeno Rapid kit, we determined its detection limit, reproducibility, cross-reactivity, and analytical reactivity for adenovirus subtypes, and performed interference studies. RESULTS: The overall agreement rates among the tested methods were 91.5% for rotavirus and 85.5% for adenovirus. On the basis of mRT-PCR, the overall agreement, positive agreement, and negative agreement rates of the ICA were 95.6%, 100%, and 94.9% for rotavirus, and 94.0%, 71.4%, and 94.8% for adenovirus, respectively. Using the ICA, we detected all the subtypes of adenovirus tested, but the analytical reactivities for adenovirus subtypes were different between the 4 adenovirus detection methods. The high reproducibility was confirmed, and no cross-reactivity or interference was detected. CONCLUSIONS: The SD BIOLINE Rota/Adeno Rapid kit showed acceptable analytical and clinical performances. However, interpretation of adenovirus positive/negative result should be cautious because of different detectability for adenovirus subtypes among adenovirus detection methods.
has issue date	2014-04-08(xsd:dateTime)
bibo:doi	10.3343/alm.2014.34.3.216
bibo:pmid	24790909
has license	cc-by-nc
sha1sum (hex)	722738d238370488034852a1867cfa04b564d331
schema:url	https://doi.org/10.3343/alm.2014.34.3.216
resource representing a document's title	Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples
has PubMed Central identifier	PMC3999320
has PubMed identifier	24790909
schema:publication	Ann Lab Med
resource representing a document's body	covid:722738d238370488034852a1867cfa04b564d331#body_text
is schema:about of	named entity 'KOREA' named entity 'CLINICAL' named entity 'DIAGNOSTICS' named entity 'HUMAN' named entity 'analytical' named entity 'Rotavirus' named entity 'Rapid' covid:arg/722738d238370488034852a1867cfa04b564d331 named entity 'SIMULTANEOUS' named entity 'DETECTION' named entity 'ASSAY' named entity 'SAMPLES' named entity 'RAPID' named entity 'STOOL' named entity 'EVALUATION' named entity 'ADENOVIRUS' named entity 'ROTAVIRUS' named entity 'RAPID' named entity 'ADENOVIRUSES' named entity 'STOOL' named entity 'PERFORMANCES' named entity 'BACKGROUND' named entity 'DETECTION' named entity 'ROTA' named entity 'SIMULTANEOUS' named entity 'ROTAVIRUSES' named entity 'STANDARD' named entity 'INC' named entity 'ANALYTICAL' named entity 'SAMPLES' named entity 'KIT' named entity 'ASSAY' named entity 'EVALUATED' named entity 'human' named entity 'Rota' named entity 'immunochromatographic' named entity 'Rota' named entity 'Samples' named entity 'Korea' named entity 'adenoviruses' named entity 'human stool' named entity 'assay' named entity 'Inc' named entity 'supernatants' named entity 'barium sulfate' named entity 'Ghana' named entity 'capsid' named entity 'real-time PCR' named entity 'adenoviruses' named entity '1:256' named entity 'hemoglobin' named entity 'stool sample' named entity 'real-time PCR' named entity 'VP6' named entity 'real-time PCR' named entity 'PCR' named entity 'adenovirus' named entity 'Korea' named entity 'ATCC' named entity 'antigens' named entity 'adenoviruses' named entity '1:2' named entity 'triglyceride' named entity 'surface antigens' named entity 'antibiotics' named entity 'Canada' named entity 'young children' named entity 'enzyme' named entity 'Korea' named entity 'fungi' named entity 'Rapid test'

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