About: Molecular and cellular evidence of natural Venezuelan equine encephalitis virus infection in frugivorous bats in Colombia

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About: Molecular and cellular evidence of natural Venezuelan equine encephalitis virus infection in frugivorous bats in Colombia Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular and cellular evidence of natural Venezuelan equine encephalitis virus infection in frugivorous bats in Colombia
Creator	Mattar, Salim Calderón, Alfonso Guzmán, Camilo Figueiredo, Moraes Tadeu, Luiz Castañeda, José Oviedo, Teresa Rodriguez, Virginia
Source	Medline; PMC
abstract	BACKGROUND AND AIM: Venezuelan equine encephalitis virus (VEEV) is an alphavirus that causes encephalitis with a high impact on public health in Latin America. However, only in Guatemala, Trinidad and Tobago, and Mexico have found antibodies in VEEV in bats, using immunohistochemistry, the sensitivity and specificity are improved; thus, it is better for demonstrating natural infection in bats as potential hosts. This study aimed to determine the presence of VEEV in tissues of frugivorous bats. MATERIALS AND METHODS: A prospective descriptive cross-sectional study with a non-probabilistic sampling was carried out in 12 localities of Córdoba and Sucre area of the Colombian Caribbean. Two hundred and eighty-six bats were captured using fog nets, and the specimens according to taxonomic keys were classified. According to the Ethics Committee of the University of Córdoba, the bats were treated with analgesics and anesthetics. Blood samples were taken and then euthanized to obtain tissues and organs which were preserved in liquid N2 at −196°C. A portion of each organ was fixed in 10% buffered formalin for the detection of antigens by immunohistochemistry. Several pathological anatomy analyses were performed to determine the histological characteristics of tissue lesions of frugivorous bats naturally infected with the VEEV. RESULTS: Of the 286 bats captured, 23 species were identified. In samples of the brain, spleen, and lung of two frugivorous bats (2/286=0.70%) Artibeus planirostris and Sturnira lilium, the presence of VEEV was confirmed by immunohistochemistry. CONCLUSION: A fragment of the nsP4 non-structural protein gene corresponding to the alphavirus was amplified. Two samples were positive (2/286=0.70%) in frugivorous bats; A. planirostris (code GenBank: MG820274) and S. lilium (code GenBank: MG820275). The present study showed the first molecular evidence and cellular evidence (histopathology and immunohistochemistry) of natural VEEV infection in frugivorous bats in Colombia; these bats could be a host of this zoonosis.
has issue date	2020-03-16(xsd:dateTime)
bibo:doi	10.14202/vetworld.2020.495-501
bibo:pmid	32367955
has license	cc-by
sha1sum (hex)	737a52242e98afc85bac937255faead79de8bad0
schema:url	https://doi.org/10.14202/vetworld.2020.495-501
resource representing a document's title	Molecular and cellular evidence of natural Venezuelan equine encephalitis virus infection in frugivorous bats in Colombia
has PubMed Central identifier	PMC7183472
has PubMed identifier	32367955
schema:publication	Vet World
resource representing a document's body	covid:737a52242e98afc85bac937255faead79de8bad0#body_text
is schema:about of	named entity 'sensitivity' named entity 'Guatemala' named entity 'Trinidad' named entity 'Mexico' named entity 'impact' named entity 'Venezuelan equine encephalitis virus' named entity 'NATURAL INFECTION' named entity 'GUATEMALA' named entity 'CAUSES' named entity 'determine' named entity 'Latin America' named entity 'tissues' named entity 'demonstrating' named entity 'equine encephalitis' named entity 'Trinidad and Tobago' named entity 'Latin America' named entity 'Mexico' named entity 'Togaviridae' named entity 'EEV' named entity 'frugivorous' named entity 'flu-like symptoms' named entity 'epizootic' named entity 'liver' named entity 'GenBank' named entity 'Human pathogens' named entity 'hematoxylin' named entity 'taxonomic identification' named entity 'preventive measures' named entity 'antigens' named entity 'immunohistochemistry' named entity 'alphaviruses' named entity 'GenBank' named entity 'cross-sectional study' named entity 'immunohistochemistry' named entity 'Venezuelan equine encephalitis virus' named entity 'VEEV' named entity 'endemic disease' named entity 'seroprevalence' named entity 'protein' named entity 'equines' named entity 'mosquitoes' named entity 'bovine serum albumin' named entity 'sample size' named entity 'histopathology' named entity 'zoonotic' named entity 'viruses' named entity 'reverse transcriptase' named entity 'Genbank' named entity 'bronchi' named entity 'histological lesions' named entity 'antigens' named entity 'infection' named entity 'epizootic' named entity 'mitochondrial gene' named entity 'viral antigens' named entity 'gliosis' named entity 'Cali' named entity '18:00' named entity 'antigen' named entity 'VEEV' named entity 'viremic' named entity 'gene' named entity 'Bat' named entity 'sodium pentobarbital' named entity 'Alphavirus' named entity 'VEEV' named entity 'lung' named entity 'arboviruses' named entity 'vectors' named entity 'lyophilized' named entity 'Venezuelan equine encephalitis virus' named entity 'suppurative' named entity 'viruses' named entity 'West Nile virus'

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