About: High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay

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About: High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay
Creator	D'aquila, Richard Demonbreun, Alexis Mcdade, Thomas Mcnally, Elizabeth Zelikovich, Aaron Bogdanovic Phd, Elena Fallon Bs, Katherine Miller Phd, Aaron Mustanski Phd, Brian Reiser Bs, Nina Vaught Bs, Lauren
Source	MedRxiv
abstract	Objective: Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). Methods: An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS. Samples were self-collected from a community sample of 232 participants enriched with health care workers, including 30 known COVID-19 cases and their household members. Results: Among 30 individuals sharing a household with a virus-confirmed case of COVID-19, 80% were seropositive. Of 202 community individuals without prior confirmed acute COVID-19 diagnoses, 36% were seropositive. Of documented convalescent COVID-19 cases from the community, 29 of 30 (97%) were seropositive for IgG antibodies to the receptor binding domain. Conclusion: DBS ELISA provides a minimally-invasive alternative to venous blood collection. Early analysis suggests a high rate of transmission among household members. High rates of seroconversion were also noted following recovery from infection. Serological testing for SARS-CoV-2 IgG antibodies in DBS samples can facilitate seroprevalence assessment in community settings to address epidemiological questions, monitor duration of antibody responses, and assess if antibodies against the spike protein correlate with protection from reinfection.
has issue date	2020-06-02(xsd:dateTime)
bibo:doi	10.1101/2020.06.01.20119602
has license	medrxiv
sha1sum (hex)	7396097259a43bfe4cf8325d68e8dfb6fee78e40
schema:url	https://doi.org/10.1101/2020.06.01.20119602
resource representing a document's title	High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay
resource representing a document's body	covid:7396097259a43bfe4cf8325d68e8dfb6fee78e40#body_text
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