About: Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes

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About: Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes
Creator	Reamtong, Onrapak Li, Ning Firczuk, Helena Hildyard, John Kannambath, Shichina Beynon, Robert Claydon, Amy Drummond, Sheona Eyers, Claire Mccarthy, John
Source	Medline; PMC
abstract	Dhh1 and Pat1 in yeast are mRNA decapping activators/translational repressors thought to play key roles in the transition of mRNAs from translation to degradation. However, little is known about the physical and functional relationships between these proteins and the translation machinery. We describe a previously unknown type of diauxic shift-dependent modulation of the intracellular locations of Dhh1 and Pat1. Like the formation of P bodies, this phenomenon changes the spatial relationship between components involved in translation and mRNA degradation. We report significant spatial separation of Dhh1 and Pat1 from ribosomes in exponentially growing cells. Moreover, biochemical analyses reveal that these proteins are excluded from polysomal complexes in exponentially growing cells, indicating that they may not be associated with active states of the translation machinery. In contrast, under diauxic growth shift conditions, Dhh1 and Pat1 are found to co-localize with polysomal complexes. This work suggests that Dhh1 and Pat1 functions are modulated by a re-localization mechanism that involves eIF4A. Pull-down experiments reveal that the intracellular binding partners of Dhh1 and Pat1 change as cells undergo the diauxic growth shift. This reveals a new dimension to the relationship between translation activity and interactions between mRNA, the translation machinery and decapping activator proteins.
has issue date	2011-06-28(xsd:dateTime)
bibo:doi	10.1093/nar/gkr474
bibo:pmid	21712243
has license	cc-by-nc
sha1sum (hex)	73aa8683fd7c4fd68b73f231b6bc3e609d4e9af9
schema:url	https://doi.org/10.1093/nar/gkr474
resource representing a document's title	Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes
has PubMed Central identifier	PMC3177209
has PubMed identifier	21712243
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:73aa8683fd7c4fd68b73f231b6bc3e609d4e9af9#body_text
is schema:about of	named entity 'DHH1' named entity 'ribosomes' named entity 'binding' named entity 'intracellular' named entity 'reveal' named entity 'activators' named entity 'PAT1' named entity 'RELOCALIZATION' covid:arg/73aa8683fd7c4fd68b73f231b6bc3e609d4e9af9 named entity 'ACTIVATOR' named entity 'TYPE' named entity 'ACTIVE' named entity 'DESCRIBE' named entity 'GROWING' named entity 'BODIES' named entity 'ACTIVITY' named entity 'NEW' named entity 'THESE' named entity 'LOCALIZE' named entity 'REVEAL' named entity 'PHYSICAL' named entity 'DEGRADATION' named entity 'INTERACTIONS' named entity 'SPATIAL' named entity 'SHIFT' named entity 'SHIFT' named entity 'COMPLEXES' named entity 'DEPENDENT' named entity 'FUNCTIONS' named entity 'TRANSLATION' named entity 'PLAY' named entity 'LOCATIONS' named entity 'FORMATION' named entity 'MACHINERY' named entity 'MECHANISM' named entity 'INVOLVED' named entity 'LITTLE' named entity 'EIF4A' named entity 'INDICATING' named entity 'EXPERIMENTS' named entity 'CELLS' named entity 'ABOUT' named entity 'COMPONENTS' named entity 'DIMENSION' named entity 'REPORT' named entity 'MODULATION' named entity 'THOUGHT' named entity 'CHANGE' named entity 'INTRACELLULAR' named entity 'RELATIONSHIPS' named entity 'UNKNOWN' named entity 'FUNCTIONAL' named entity 'MRNA DEGRADATION' named entity 'PROTEINS' named entity 'ASSOCIATED WITH' named entity 'RELATIONSHIP' named entity 'KEY' named entity 'SEPARATION' named entity 'BIOCHEMICAL' named entity 'DHH1' named entity 'CHANGES' named entity 'EXCLUDED' named entity 'PAT1' named entity 'FOUND' named entity 'PREVIOUSLY' named entity 'WORK' named entity 'GROWTH' named entity 'RIBOSOMES' named entity 'MODULATED' named entity 'SIGNIFICANT' named entity 'PHENOMENON' named entity 'ROLES'

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