About: Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation

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About: Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation
Creator	Park, Chan Kim, Paul Lee, S Lee, C Chae, Wonil Choi, Y Choi, Seong Jang, Yo Kim, Choon Kim, Y Kim, Young Kwon, Soon Lee, Yoon Lee, Young Seong, Baik Yang, Seung Chae, Y Ho Byun, Young Jang, W Kim, C Kwon, Y Park, P
Source	PMC
abstract	A novel protein-folding function of RNA has been recognized, which can outperform previously known molecular chaperone proteins. The RNA as a molecular chaperone (chaperna) activity is intrinsic to some ribozymes and is operational during viral infections. Our purpose was to test whether influenza hemagglutinin (HA) can be assembled in a soluble, trimeric, and immunologically activating conformation by means of an RNA molecular chaperone (chaperna) activity. An RNA-interacting domain (RID) from the host being immunized was selected as a docking tag for RNA binding, which served as a transducer for the chaperna function for de novo folding and trimeric assembly of RID-HA1. Mutations that affect tRNA binding greatly increased the soluble aggregation defective in trimer assembly, suggesting that RNA interaction critically controls the kinetic network in the folding/assembly pathway. Immunization of mice resulted in strong hemagglutination inhibition and high titers of a neutralizing antibody, providing sterile protection against a lethal challenge and confirming the immunologically relevant HA conformation. The results may be translated into a rapid response to a new influenza pandemic. The harnessing of the novel chaperna described herein with immunologically tailored antigen-folding functions should serve as a robust prophylactic and diagnostic tool for viral infections.—Yang, S. W., Jang, Y. H., Kwon, S. B., Lee, Y. J., Chae, W., Byun, Y. H., Kim, P., Park, C., Lee, Y. J., Kim, C. K., Kim, Y. S., Choi, S. I., Seong, B. L. Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation.
has issue date	2018-01-08(xsd:dateTime)
bibo:doi	10.1096/fj.201700747rr
bibo:pmid	29295864
has license	cc-by-nc
sha1sum (hex)	73d22ae218e1b28a39ac87844927cc56f164cbc2
schema:url	https://doi.org/10.1096/fj.201700747rr
resource representing a document's title	Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation
has PubMed Central identifier	PMC5901386
has PubMed identifier	29295864
schema:publication	FASEB J
resource representing a document's body	covid:73d22ae218e1b28a39ac87844927cc56f164cbc2#body_text
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