About: Differentially expressed genes in human gingival fibroblasts cultured on microgrooved titanium substrata: A pilot study

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Differentially expressed genes in human gingival fibroblasts cultured on microgrooved titanium substrata: A pilot study Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Differentially expressed genes in human gingival fibroblasts cultured on microgrooved titanium substrata: A pilot study
Creator	Ahn, Su Kim, Kyung Kang, Hyun Jung, Su Kwon, Il Lee, Suk Leesungbok, Richard Yang, Dae
source	PMC
abstract	The purpose of this study was to determine the differentially expressed genes in human gingival fibroblasts (HGFs) cultured on titanium (Ti) substrata with topographies presenting microgrooves and acid-etched roughness. Microgrooves were fabricated with a truncated V-shape in cross-section at 15/3.5, 30/10, and 60/10 μm (width/depth) by photolithography. Subsequent acid etching was applied to the entire surface of the fabricated Ti substratum to generate etched microgrooves and ridges (designated as E15/3.5, E30/10, and E60/10). Both smooth and acidetched-only Ti were used as controls (designated as NE0 and E0). Large-scale gene expression analyses were performed using differential display PCR, and the results were confirmed using RT-PCR and quantitative real-time PCR. Of the 21 genes with altered expression determined by differential display PCR and sequencing, we verified through RT-PCR that MTDH and TIMP1 were up-regulated and TGF-β1, TPM1, and VIM were down-regulated in the HGFs cultured on E60/10 versus NE0. We also confirmed, by quantitative real-time PCR, that MTDH and TIMP1 expression in HGFs on E60/10 was significantly up-regulated compared to HGFs on the other Ti substrata. This study indicates that acid-etched ridges and microgrooves on Ti with a width and depth of 60 and 10 μm (E60/10) induce alterations in the expression of genes involved in cell adhesion, proliferation, and regulation of the cytoskeleton in HGFs.
has issue date	2012-03-27(xsd:dateTime)
bibo:doi	10.1007/s13770-012-0020-x
has license	no-cc
sha1sum (hex)	73f519529a9fddda957d90c5c55dd2d91a206d1e
schema:url	https://doi.org/10.1007/s13770-012-0020-x
resource representing a document's title	Differentially expressed genes in human gingival fibroblasts cultured on microgrooved titanium substrata: A pilot study
has PubMed Central identifier	PMC7100679
schema:publication	Tissue Eng Regen Med
resource representing a document's body	covid:73f519529a9fddda957d90c5c55dd2d91a206d1e#body_text
is schema:about of	named entity 'gene' named entity 'E60' named entity 'induce' named entity 'controls' named entity 'TPM1' named entity 'confirmed' named entity 'alterations' named entity 'cytoskeleton' named entity 'Genes' named entity 'SURFACE' named entity 'CONTROLS' named entity 'TPM1' named entity 'ROUGHNESS' named entity 'DEPTH' named entity 'SUBSTRATA' named entity 'COMPARED' named entity 'RT-PCR' named entity 'USED' named entity 'PRESENTING' named entity 'HUMAN GINGIVAL FIBROBLASTS' named entity 'PCR' named entity 'ACID ETCHING' named entity 'E15' named entity 'ALTERATIONS' named entity 'E60' named entity 'study' named entity 'E60' named entity 'TGF-β1' named entity 'fibroblasts' named entity 'expression' named entity 'expressed genes' named entity 'PCR' named entity 'truncated' named entity 'quantitative real-time PCR' named entity 'cultured' named entity 'differential display' named entity 'E15' named entity 'TPM1' named entity 'TIMP1' named entity 'up-regulated' named entity 'TGF-β1' named entity 'gene expression' named entity 'quantitative real-time PCR' named entity 'differentially expressed genes' named entity 'TIMP1' named entity 'VIM' named entity 'Substrata' named entity 'Fibroblasts' named entity 'Pilot Study' named entity 'differential display' named entity 'isoforms' named entity 'ANOVA' named entity 'gene cloning' named entity 'down-regulated' named entity 'astrocyte' named entity 'E15' named entity 'gene' named entity 'gene' named entity 'Vimentin' named entity 'mRNA expression' named entity 'wisdom teeth' named entity 'cell adhesion' named entity 'TIMP1' named entity 'differential display' named entity 'PCR' named entity 'Bio-Rad Laboratories' named entity 'patellar tendon' named entity 'down-regulation' named entity 'Serum levels' named entity 'synergistic effect' named entity 'expression patterns' named entity 'Differential display' named entity 'cDNA' named entity 'muscle cells' named entity 'gene' named entity 'TGF-β1'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software